Chemical castration using an intratesticular injection of mannitol: a preliminary study in a rat model

MAADI, Mohammad-Ali; Behfar, Mehdi; RASAEI, Amir; Jalali, Ali Shalizar; Najafi, Gholamreza; Mohammadi, Vahid

doi:10.3906/vet-2010-111

Cited by 4 publications

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“…Our study found no observed side effects in the animals after intratesticular HM and HS in donkey testis. Similar to our finding, a previous study previous study found that side effects such as diarrhea, lethargy, vomiting, scrotal ulceration, and dermatitis were not observed following intratesticular HM injection [ 29 ].…”

Section: Discussionsupporting

confidence: 92%

“…8 ). In mammals, intratesticular injection of calcium chloride, zinc gluconate, HM, and HS have all been tested for chemical castration [ 3 , 16 , 17 , 29 ]. Animals have been reported to be chemically, surgically and mechanically castrated [ 13 , 59 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

“…Fifteen adult male donkeys were randomly assigned to one of five treatment groups: (1) Control group (no treatment), (2) Surgery group (surgical castration for testosterone control), (3) NS group (receiving a single bilateral intratesticular injection (20 mL per testis) of normal saline (sodium chloride 0.9%, Iran Injection and Pharmaceutical Products Company, Tehran, Iran), (4) HS group (receiving a single bilateral intratesticular injection of sodium chloride 20%, Iran Injection and Pharmaceutical Products Company, Tehran, Iran), and (5) HM group (receiving a single bilateral intratesticular injection of HM 20% (Iran injection and pharmaceutical products company, Tehran, Iran) (Fig. 1 ), [ 3 , 29 ].

Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Administration of hypertonic saline can induce acute local osmotic shock and result in widespread necrosis. Also, this approach was effective in sterilization in adult canine and rat models [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…HM, a sugar alcohol, is incompletely metabolized and is excreted in its native form through the urinary tract. Maadi et al (2021) demonstrated that single intratesticular injection of HM resulted in sterilizations in rats [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Baqerkhani,

Soleimanzadeh,

Mohammadi

2024

BMC Vet Res

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Objectives The aim of the present study was to examine donkey sperm quality after intratesticular injection of hypertonic mannitol (HM) and saline (HS). Methods Randomly assigned to five treatment groups were 15 adult male donkeys: (1) Control group (no treatment), (2) Surgery group (surgical castration for testosterone control), (3) NS group (normal saline intratesticular injection), (4) HS group (hypertonic saline), and (5) HM group. We injected 20 mL per testicle. We took 5 mL blood from all donkeys before injection. Castration was performed under general anesthesia 60 days later. Samples included blood and testicular tissue. Total motility (TM), progressive motility (PM), movementy features, DNA damage, morphology, viability, and plasma membrane functionality were evaluated. Hormone analyses, histomorphometric studies and oxidative stress indices including total antioxidant capacity (TAC), glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), and NADP+/NADPH were evaluated. Apoptosis, pyroptosis-related Bax, Caspase-1, GSDMD, and Bcl-2 expression were also assessed. Results In HS and HM groups, testosterone, epididymal sperm count, motility, viability, and plasma membrane functionality dropped while sperm DNA damage increased. HS and HM groups had significantly lower histomorphometric parameters, TAC, GPx, SOD, GSH, and Bcl-2 gene expression. MDA, NADP+/NADPH, Bax, Caspase-1, and GSDMD gene expression were substantially higher in the HS and HM groups than in the control group. Conclusions Toxic effects of hypertonic saline and mannitol on reproductive parameters were seen following, hence, they might be considered as a good chemical sterilizing treatment in donkeys.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Fig.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Baqerkhani,

Soleimanzadeh,

Mohammadi

2024

BMC Vet Res

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Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications

Coimbra

Dantas

Andrade³

et al. 2023

Toxicology

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Efficacy of silver-doped Carbon dots in Chemical Castration: a rat model study

Soleimanzadeh,

Karvani,

Davoodi

et al. 2024

Sci Rep

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This study evaluates silver-doped carbon dots (AgCDs) as a novel agent for chemical castration using a rat model. Six groups of rats (five males and ten females each, except for the surgical group which had only males) were utilized to compare the effects of different concentrations of AgCDs. The groups included control, sham, and three experimental groups injected with 1.25, 50, and 200 µg/mL AgCDs, respectively, along with a surgical castration group. Testosterone levels, sperm parameters, fertility index, oxidative damage, histopathological parameters, and gene expression of P53 , Bax , Bcl-2 , caspase-3 , AKT , and PI3K were analyzed. Results demonstrated that the high-dose AgCDs group significantly reduced testosterone levels, sperm concentration, and motility, resulting in a decreased fertility index. MDA and NO significantly increased, while CAT, SOD, GPx, and TAC significantly reduced in the chemically castrated groups. Histological and genes expression analysis also revealed apoptosis and testicular damage in the AgCDs groups, indicated by significant increases in P53 , Bax , and Caspase-3 levels, and significant reductions in AKT , PI3K , and Bcl-2 . Based on these findings, AgCDs could be considered a potent and efficient agent for chemical castration, offering a less invasive, cost-effective solution with potential applications for population control.

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Chemical castration using an intratesticular injection of mannitol: a preliminary study in a rat model

Cited by 4 publications

References 0 publications

Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications

Efficacy of silver-doped Carbon dots in Chemical Castration: a rat model study

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