Chemical characteristics and cytoprotective activities of polysaccharide fractions from Athyrium Multidentatum (Doll.) Ching

Liang, Jing; Sheng, Jian‐Zhong; Jiang, Jingru; Wang, Yang; Shen, X. Y.; Li, Dongmei; Zhang, Weifen; Mao, Shumei

doi:10.1016/j.ijbiomac.2020.05.053

Cited by 16 publications

(31 citation statements)

References 38 publications

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“…This finding was consistent with the previous physicochemical-property analysis [ 35 ]. As shown in Figure 1 B, a broad and strong absorption peak at 3410 cm −1 was attributed to the O–H stretching vibration, and a relatively weak absorption peak at 2910 cm −1 was assigned to the C–H stretching vibration [ 36 ]. The absorption peak at 1646 cm −1 might be caused by the O–H bending vibration [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

Xiao

Xie

et al. 2022

Foods

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This study aimed to characterize the structure of Chinese yam (Dioscoreae Rhizoma) polysaccharide (CYP) and to investigate its protective effect against H2O2-induced oxidative damage in IEC-6 cells. The chemical composition and structural characteristics of the samples were analyzed by chemical and instrumental methods, including high-performance gel permeation chromatography, high-performance anion-exchange chromatography (HPAEC), Fourier transformed infrared (FT-IR), ultraviolet (UV), and scanning electron microscopy (SEM). Antioxidant activity was evaluated by establishing a cellular model of oxidative damage. The molecular weight of CYP was 20.89 kDa. Analysis of the monosaccharide composition revealed that CYP was primarily comprised of galactose (Gal), glucose (Glu), and galacturonic acid (GalA), and the ratio between them was 28.57:11.28:37.59. Pretreatment with CYP was able to improve cell viability, superoxide dismutase (SOD) activity, and reduce intracellular reactive oxygen species (ROS) production and malondialdehyde (MDA) content after H2O2 injury. CYP also attenuated oxidative damage in cells through the mitogen-activated protein kinase (MAPK) signaling pathway. This study showed that CYP was an acidic heteropolysaccharide with a good protective effect against oxidative damage, and it thus has good prospects in food and biopharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

Xiao

Xie

et al. 2022

Foods

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“…Infrared spectroscopy is usually used to analyze the functional groups, monosaccharide types and glycosidic linkages of polysaccharides [ 30 ]. The FT-IR spectra of AVPG-1 and AVPG-2 are shown in Figure 3 C. The FT-IR spectra could be divided into five fractions ( A , B , C , D and E ).…”

Section: Resultsmentioning

confidence: 99%

Purification, Structural Characterization and Immunomodulatory Effects of Polysaccharides from Amomumvillosum Lour. on RAW 264.7 Macrophages

et al. 2021

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Amomum Villosum Lour. (A. villosum) is a folk medicine that has been used for more than 1300 years. However, study of the polysaccharides of A. villosum is seriously neglected. The objectives of this study are to explore the structural characteristics of polysaccharides from A. villosum (AVPs) and their effects on immune cells. In this study, the acidic polysaccharides (AVPG-1 and AVPG-2) were isolated from AVPs and purified via anion exchange and gel filtration chromatography. The structural characteristics of the polysaccharides were characterized by methylation, HPSEC-MALLS-RID, HPLC, FT-IR, SEM, GC-MS and NMR techniques. AVPG-1 with a molecular weight of 514 kDa had the backbone of → 4)-α-d-Glcp-(1 → 3,4)-β-d-Glcp-(1 → 4)-α-d-Glcp-(1 →. AVPG-2 with a higher molecular weight (14800 kDa) comprised a backbone of → 4)-α-d-Glcp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-d-Glcp-(1 →. RAW 264.7 cells were used to investigate the potential effect of AVPG-1 and AVPG-2 on macrophages, and lipopolysaccharide (LPS) was used as a positive control. The results from bioassays showed that AVPG-2 exhibited stronger immunomodulatory activity than AVPG-1. AVPG-2 significantly induced nitric oxide (NO) production as well as the release of interleukin (IL)-6 and tumor necrosis factor alpha (TNF-α), and upregulated phagocytic capacities of RAW 264.7 cells. Real-time PCR analysis revealed that AVPG-2 was able to turn the polarization of macrophages to the M1 direction. These results suggested that AVPs could be explored as potential immunomodulatory agents of the functional foods or complementary medicine.

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“…Numerous studies hinted that the PI3K/Akt signaling pathway protects against D-gal-induced brain injury by modulating Nrf2 46, 47 . Additionally, several natural compounds were found to improve the behavioral dysfunction and neurological de cits in D-galactose-induced aging in mice via activation of PI3K/Akt/Nrf2 pathway [48][49][50] . Results of the current work demonstrated that phosphorylated levels of PI3K and Akt are signi cantly upregulated after GNL treatment, which revealed that GNL can activate PI3K/Akt signaling pathway to promote Nrf2 nuclear translocation via reduction D-galactose-induced oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Geraniol Attenuates Oxidative Stress and Neuro-inflammation Mediated Cognitive Impairment in D‑galactose‑induced Mouse Aging Model

Rajendran

Ammar

Al-Saeedi

et al. 2022

Preprint

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D-galactose (D-gal) is a reducing sugar drug can induce artificial senescence and aging process that mimic natural aging along with the accompanying brain and liver injury in experimental animals. Therefore, chronic D-gal administration is widely used to induce cognitive impairment, Alzheimer disease and aging in rodents' models. Aging is a phenomenon in which oxidative stress and apoptosis play a vital role. Geraniol (GNL) belongs to the acyclic isoprenoid monoterpenes, presents in essential oils such as those from Cinnamomum tenuipilum and Valeriana officinalis. In the present study, we examined the effects of GNL on D-gal-induced oxidative stress and neuro-inflammation mediated memory loss in mice. Analyzing the behavioral differences between control and treated groups, including the elderly mice, revealed that GNL significantly improved memory in mice treated with D-gal-induced memory loss (supplementary videos are provided). The anti-inflammatory and the anti-oxidative role of GNL were confirmed by both histopathological investigations and biochemical analyses. Mechanistically, GNL appears to activate PI3K/Akt and thus upregulates the nuclear factor erythroid 2-related factor 2 (Nrf2) and the heme oxygenase 1 (HO-1) to reduce the oxidative stress and apoptosis induced after D-gal treatment leading to easing of neurological deficits and cognitive dysfunction in D-gal-induced aging mouse models. Accordingly, our comprehensive behavioral analysis and bioassays suggest GNL as a promising agent preventing cognitive impairment and neurological deficits associated with aging.

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Chemical characteristics and cytoprotective activities of polysaccharide fractions from Athyrium Multidentatum (Doll.) Ching

Cited by 16 publications

References 38 publications

Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

Purification, Structural Characterization and Immunomodulatory Effects of Polysaccharides from Amomumvillosum Lour. on RAW 264.7 Macrophages

Geraniol Attenuates Oxidative Stress and Neuro-inflammation Mediated Cognitive Impairment in D‑galactose‑induced Mouse Aging Model

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