Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2

Prathyusha, A. M. V. N.; Sheela, G. Mohana; Bramhachari, Pallaval Veera

doi:10.1016/j.btre.2018.e00277

Cited by 31 publications

(14 citation statements)

References 41 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The signal at 1072.42 cm -1 was obtained from glycosidic bonds C-O-C vibration. A typical absorption at 894.97 cm -1 suggested the presence of α-anomeric in carbohydrate molecules (Zhbankov 1997;Prathyusha et al 2018). Therefore, the FT-IR spectra of EPS by the occurrence of carboxyl, carbonyl, glycosidic bonds, and hydroxyl confirmed the characteristics of polysaccharides.…”

Section: Resultsmentioning

confidence: 75%

Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

et al. 2021

View full text Add to dashboard Cite

Abstract. Yurnaliza Y, Jamilah I, Hartanto A, Lutfia A. 2021. Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides. Biodiversitas 22: 1467-1473. Exopolysaccharides (EPS) are microbial polysaccharides with a high biotechnological application in many fields due to their bioactivity and biocompatibility. Endophytic fungi are potential agents for producing EPS even though they remained under-exploited, especially those originating as indigenous strains from oil palm (Elaeis guineensis Jacq.). The present study reports on EPS production by a collection of endophytic fungi isolated from the petioles of oil palm under submerged fermentation. The culture medium was formulated with sucrose as C-source to trigger the secretion of EPS by the strains. Isolation of EPS was done through absolute ethanol precipitation in cold conditions. A total of 29 endophytic fungal strains was recovered from the foliar part of the plants. Molecular identification based on ITS-rDNA region and phylogenetic construction of each lineage revealed the dominant species from Pestalotiopsidaceae (syn. Amphisphaeriaceae), followed by Hypoxylaceae, Pleosporaceae, Didymosphaeriaceae, Sporocadaceae, and other minor families. In general, most isolates were ascomycetous fungi along with a basidiomycetous and zygomycetous fungus. After fermentation for 10 days, three fungal endophytes identified as Annulohypoxylon thailandicum P8 (Hypoxylaceae), Pseudopestalotiopsis simitheae P1 (Pestalotiopsidaceae), and Diaporthe eucalyptorum P11 (Diaporthaceae) secreted EPS higher in values than the others. The most prominent strain was A. thailandicum P8 with a yield of >50 mg/100 mL of filtrate solution. Based on FT-IR spectral analysis, the EPS product was detected to contain carboxyl, carbonyl, glycosidic bonds, and hydroxyl groups hereafter confirmed the characteristics of exopolysaccharides. The results demonstrated that the EPS may be evaluated for its bioactivity in a future study.

show abstract

Section: Resultsmentioning

confidence: 75%

Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The FT-IR spectra of EPSs produced from Fusarium equiseti ANP2 showed the occurrence of carboxyl, hydroxyl, carbonyl groups, and glycosidic bonds [47]. The carboxyl groups in EPSs attack the reactive site at divalent cations and exhibit sequestration [47].…”

Section: Discussionmentioning

confidence: 99%

Optimizations of exopolysaccharide production by Fusarium nygamai strain AJTYC1 and its potential applications as an antioxidant, antimicrobial, anticancer, and emulsifier

El-Mahdy,

Mohamed,

El-Ansary

2023

BMC Microbiol

View full text Add to dashboard Cite

Background Exopolysaccharides (EPSs) produced by microbes are recognized as biomacromolecules of great significance. EPSs from fungi are widely used in a variety of biotechnological fields, including medicine, bioremediation, and agriculture. Results In this study, ten fungal isolates were isolated from Kafir El-Dair, Qalubia Governorate, Egypt. Isolate 5 produced more exopolysaccharides than the other examined fungi. According to microscopic morphological traits and genetic confirmation by the 18S rRNA gene, isolate 5 was identified as Fusarium nygamai strain AJTYC1. The present study showed that Czapek’s broth media, which contains 6 g/100 ml of sucrose, 10 g/100 ml of peptone, pH 6, and 1.8 × 105 CFU/ml of inoculum size and is incubated at 30 °C for 9 days, was suitable for the production of EPSs from Fusarium nygamai strain AJTYC1 by using static conditions. Fourier transform infrared (FT-IR) was employed in the characterization of EPSs, which exhibited the presence of carboxyl groups, hydroxyl groups, carbonyl groups, and glycosidic bonds. High-performance liquid chromatography (HPLC) detected that EPSs consist of sucrose and glucose. The scavenging activity indicates that EPSs have good antioxidant activity. The partially purified exopolysaccharides produced from F. nygamai strain AJTYC1 exhibited excellent antioxidant and antimicrobial activity against gram positive, gram negative and fungal strains. The EPSs at a dose of 1000 µg/ml exhibited anticancer activity against colorectal colon cancer (HCT116), breast cancer (MCF7), and hepatocellular cancer cell lines. Moreover, EPSs is an effective emulsifier of a variety of vegetable oils, and the emulsion it produces is generally stable for up to 168 h. Conclusions The production of EPSs from F. nygamai strain AJTYC1 can be used as antioxidants, antimicrobials, anticancer, and emulsifiers.

show abstract

“…The polysaccharide of this F. solani strain most likely being homogeneous, as only one peak was observed in the chromatogram [6]. Mahapatra and Banerjee [44] described 190 kDa EPS of another Fusarium strain belonging to the F. solani species whose polysaccharide turned out to be a heteropolymer consisting of rhamnose and galactose. The type of linkages and percentage of glycosidic bonds in the polysaccharidic components of EPSs obtained from F. culmorum strains cultures was very diverse (Table 3).…”

Section: Molecular Weights Of Eps Polysaccharidic Fractions Glycosidmentioning

confidence: 93%

Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of Endophytic Fusarium culmorum Strains with Different Effects on Cereals

et al. 2020

View full text Add to dashboard Cite

Exopolymeric substances (EPS) can determine plant-microorganism interactions and have great potential as bioactive compounds. The different amounts of EPS obtained from cultures of three endophytic Fusarium culmorum strains with different aggressiveness—growth promoting (PGPF), deleterious (DRMO), and pathogenic towards cereal plants—depended on growth conditions. The EPS concentrations (under optimized culture conditions) were the lowest (0.2 g/L) in the PGPF, about three times higher in the DRMO, and five times higher in the pathogen culture. The EPS of these strains differed in the content of proteins, phenolic components, total sugars, glycosidic linkages, and sugar composition (glucose, mannose, galactose, and smaller quantities of arabinose, galactosamine, and glucosamine). The pathogen EPS exhibited the highest total sugar and mannose concentration. FTIR analysis confirmed the β configuration of the sugars. The EPS differed in the number and weight of polysaccharidic subfractions. The EPS of PGPF and DRMO had two subfractions and the pathogen EPS exhibited a subfraction with the lowest weight (5 kDa). The three EPS preparations (ethanol-precipitated EP, crude C, and proteolysed P) had antioxidant activity (particularly high for the EP-EPS soluble in high concentrations). The EP-EPS of the PGPF strain had the highest antioxidant activity, most likely associated with the highest content of phenolic compounds in this EPS.

show abstract

Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2

Abstract: Graphical abstract

Cited by 31 publications

References 41 publications

Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

Optimizations of exopolysaccharide production by Fusarium nygamai strain AJTYC1 and its potential applications as an antioxidant, antimicrobial, anticancer, and emulsifier

Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of Endophytic Fusarium culmorum Strains with Different Effects on Cereals

Contact Info

Product

Resources

About