Chemical characterization, computational analysis and biological views on Daphne gnidioides Jaub. &amp; Spach extracts: Can a new raw material be provided for biopharmaceutical applications?

Can, Tevfik Hasan; Tüfekci, Enis Fuat; Altunoğlu, Yasemin Çelik; Baloğlu, Mehmet Cengiz; Llorent‐Martínez, Eulogio J.; Stefanucci, Azzurra; Mollica, Adriano; Cichelli, Angelo; Zengin, Gökhan

doi:10.1016/j.compbiolchem.2020.107273

Cited by 10 publications

(6 citation statements)

References 58 publications

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“…Safe, new, and clinically effective approaches are needed to overcome these side effects and treatment limitations. Plants offer hopeful results in fighting cancer as they have a resource of extraordinary chemical structures and a broad range of biological activities [59,60] . In this study, cytotoxic effects of the ethyl acetate, methanol and water extracts of H. humile were tested on variable breast cancer cell lines by MTT assay.…”

Section: Resultsmentioning

confidence: 99%

Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound‐Assisted Extraction Method

Öçal

Altunoğlu

Angeloni

et al. 2022

Chemistry & Biodiversity

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As the safety and effectiveness of synthetic drugs remain in doubt, researchers are trying to develop natural medicines from medicinal plants. Herein, ethyl acetate, methanol and water extracts from the Heracleum humile plant were obtained by an ultrasonic‐assisted extraction process and the aim was to evaluate some biological effects of the extracts due to the limited data on the pharmacological properties of Heracleum humile in the literature. Weak antibacterial activity was observed on tested bacterial species. The minimum inhibitory concentration and the minimum bactericidal concentration values ranged from 250 to 500 μg/mL. In addition, cytotoxic activity was determined using the MTT test. The strongest findings were determined for ethyl acetate extract on the MDA‐MB‐231 cell lines at the 48th hour (IC50:97.94 μg/mL), followed by the MCF‐7 cell lines at the 24th hour (IC50:103.9 μg/mL). All extracts of Heracleum humile contained mainly flavonoids, phenolic acids and their derivatives, i. e., well‐known compounds that possess numerous biological activities such as antioxidant, anti‐inflammatory, anticancer, antimicrobial etc. The study results could provide important information that Heracleum humile could be a potential candidate as a natural enzyme inhibitor. It can be concluded that these extracts could be useful in the elementary step of improving novel plant‐derived multifunctional pharmaceuticals.

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Section: Resultsmentioning

confidence: 99%

Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound‐Assisted Extraction Method

Öçal

Altunoğlu

Angeloni

et al. 2022

Chemistry & Biodiversity

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“…The phytochemical profiling of Senna occidentalis root extracts presented in this study revealed a diverse range of the bioactive phenolics compound. Some of the identified bioactive phenolics were reported by several authors for antidiabetic activities; Valle et al [ 46 ], Can et al [ 47 ], and Mohammed et al [ 48 ]. Besides, the larvicidal activity was carried out against Culex quinquefasciatus mosquito larvae, which showed that the methanol extract showed moderate effects and n-hexane and ethyl acetate extracts showed safe effects.…”

Section: Resultsmentioning

confidence: 99%

Physiochemical, Insecticidal, and Antidiabetic Activities of Senna occidentalis Linn Root

Issa

Ahmed

Mohamed

et al. 2020

Biochemistry Research International

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The present study aimed to investigate the physiochemical activities of Senna occidentalis (Linn) roots and phytochemicals as insecticidal (ethyl acetate and methanol) and antidiabetic (ethanolic extract) activities. Physicochemical properties were carried out by using Association of Official Analytical Chemist methods; thin layer chromatography was carried out according to the Stahl method. Larvicidal activity and LD50 were studied against the third instar of Culex quinquefasciatus mosquito larvae to detect and extract toxicity. The ethanolic extracts of the roots were orally tested at the dose 200 mg/kg for the hypoglycemic effect on induced hyperglycemia in normal rats, assessed in the ethanol extract, and were compared with diabetic control and standards glibenclamide 10 mg/kg. Physiochemical parameters showed high rate in the nitrogen-free extract (69.6%), curd fiber (14.5%), crude proteins (8.15%), ether extract (3.75%), and both ash and moisture (2%), and high concentrations values were found in potassium (43 mg/l) followed by phosphorous (28.5 mg/l), calcium (15 mg/l), sodium (3.65 mg/l), and magnesium (0.145 mg/l). In this part, phytochemical compounds showed high amount of alkaloids, triterpene, flavonoids, tannins, sugars, and few amount of anthraquinone glycosides. Thin-layer chromatography (TLC) studies different colored phytochemical constituted with different Rf values. All the spots are colored under UV light, but some are localized colorless after spaying. The ethyl acetate (EtAc) extract showed eight spots, and the methanol (MeOH) extract showed thirteen spots. The larvicidal activity showed that the ethyl acetate extract was safe against mosquito larvae with an LD50 value 1412.54 (p<0.05), and the methanol extract had moderate larvicidal activity against mosquito larvae with an LD50 value 257.54 (p<0.05), while the ethanolic extract of Senna occidentalis (L.) causes a favorable hypoglycemic activity when compared to control significant reduction by [53.15%, 32.87%, and 20.94%], respectively, as well as standard glibenclamide. Based on the various data of the physicochemical parameters, TLC spots, and phytochemical compounds of Senna occidentalis root, they could be used as references standards for manufacturing units of Senna occidentalis root larvicidal and antidiabetic drugs.

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“…In vitro cell survival analyzes were performed by MTT assay as described before with slight modifications [ 30 ]. Cells at the exponential growth phase were cultured on 96-well plates at the seeding density of 1.0 × 10 4 cells per well.…”

Section: Methodsmentioning

confidence: 99%

LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of Boerhavia diffusa Extracts: A Potential Raw Material for Functional Applications

et al. 2021

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Boerhavia diffusa is a great tropical plant and is widely used for various traditional purposes. In the present study, we examined the influence of solvents (dichloromethane, ethyl acetate, methanol and infusion (water)) on chemical composition and biological capabilities of B. diffusa. An UHPLC-HRMS method was used to determine the chemical characterization. The biological ability was examined for antioxidant, enzyme inhibitory and anti-cancer effects. To evaluate antioxidant effects, different chemical methods (ABTS, DPPH, CUPRAC, FRAP, metal chelating and phosphomolybdenum) were applied. With regard to enzyme inhibitory properties, cholinesterases, amylase, glucosidase and tyrosinase were used. The MDA-MB-231 breast cancer cell line was chosen to determine anticancer activity. Based on the UHPLC-HRMS analysis, 37 specialized metabolites were dereplicated and identified in the studied extracts. Results revealed the presence of 15 hydroxybenzoic, hydroxycinnamic, acylquinic acids, and their glycosides, one rotenoid, seven flavonoids, 12 fatty acids and two other glycosides. Among the tested extracts, the methanol extract showed a stronger antioxidant ability compared with other extracts. The methanol extract also showed the best inhibitory effects on tyrosinase and glucosidase. In the anti-cancer evaluation, the methanol extract showed stronger anticancer effects compared with water extract. In summary, our observations can contribute to the establishment of B. diffusa as a potential candidate for functional applications in the preparation.

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Chemical characterization, computational analysis and biological views on Daphne gnidioides Jaub. & Spach extracts: Can a new raw material be provided for biopharmaceutical applications?

Cited by 10 publications

References 58 publications

Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound‐Assisted Extraction Method

Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound‐Assisted Extraction Method

Physiochemical, Insecticidal, and Antidiabetic Activities of Senna occidentalis Linn Root

LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of Boerhavia diffusa Extracts: A Potential Raw Material for Functional Applications

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