Chemical characterization of small‐molecule inhibitors of monoamine oxidase B synthesized from the <scp><i>Acanthopanax senticosus</i></scp> root with affinity ultrafiltration mass spectrometry

He, Yingkun; Wang, Yimin; Zhang, Xin; Zheng, Zhong; Liu, Shu; Xing, Junpeng; Liu, Zhi‐Qiang; Zhou, Hui

doi:10.1002/rcm.8694

Cited by 18 publications

(13 citation statements)

References 30 publications

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“…is is consistent with the findings that the root of A. senticosus could potentially be used in the management of neurodegenerative diseases [29].…”

Section: Effects Of Differentsupporting

confidence: 92%

Different Metabolites in the Roots, Seeds, and Leaves of Acanthopanax senticosus and Their Role in Alleviating Oxidative Stress

Wang²,

et al. 2021

Journal of Analytical Methods in Chemistry

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In this study, we examined the metabolites from different parts of Acanthopanax senticosus and their role in alleviating damage caused by oxidative stress. We used UHPLC-QTOF-MS to analyze the chemical components in the root, seed, and leaf extracts of A. senticosus. Two multivariate statistical analysis methods—namely, principal component analysis and partial least square discriminant analysis—were used to distinguish the samples obtained from different parts of the plant. Using univariate statistics, 130 different metabolites were screened out. Among these, the relative content of flavonoids and terpenoids was found to be highest in the leaves, the lignin and phenolic acid content was highest in the roots, and the amino acid and phenolic acid levels were highest in seeds. An MTT assay was used to test the anti-H2O2 oxidative damage to PC12 cells in different parts of the sample. Lastly, using Pearson’s correlation analysis, various metabolites from different parts of A. senticosus were correlated with their antioxidant effects from the corresponding parts. Fifty-two related different metabolites were found, of which 20 metabolites that were positively correlated to oxidative stress were present at a relatively higher level in the roots, whereas 32 metabolites that were negatively correlated were present at relatively higher levels in the seeds and leaves. The results of this study reveal the distribution characteristics and the antioxidant activity of different metabolites of A. senticosus and provide a reference for the rational development of its medicinal parts.

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“…is is consistent with the findings that the root of A. senticosus could potentially be used in the management of neurodegenerative diseases [29].…”

Section: Effects Of Differentsupporting

confidence: 92%

Different Metabolites in the Roots, Seeds, and Leaves of Acanthopanax senticosus and Their Role in Alleviating Oxidative Stress

Wang²,

et al. 2021

Journal of Analytical Methods in Chemistry

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“…For quinic acid, one previous study described the ability of this molecule to protect rats from aluminum chloride-induced dementia and suggested non-selective inhibition of MAO as a potential mechanism [ 38 ]. The selectivity of caffeoylquinic acid derivatives is unclear: two previous articles [ 37 , 39 ] provide evidence for selective activity against MAO-B, whereas another [ 40 ] also showed an effect against MAO-A.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Human Monoamine Oxidases A and B by Specialized Metabolites Present in Fresh Common Fruits and Vegetables

Marzo

Gambini

Poletti

et al. 2022

Plants

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Diets rich in fruits and vegetables are associated with better psychological wellbeing and cognitive functions, although it is unclear which molecules and mechanisms are involved. One potential explanation is the inhibition of monoamine oxidases (MAOs), which have been linked to several neurological disorders. The present study investigated the ability of kiwifruit to inhibit MAO-A and MAO-B, refining an in vitro assay to avoid confounding effects. Ultra-performance liquid chromatography/mass spectrometry (UPLC-QTOF) and nuclear magnetic resonance spectroscopy (NMR) were used to select individual kiwifruit metabolites for further analysis. Moreover, extracts of other common fruits and vegetables were screened to identify promising candidate inhibitors. Multiple extracts and compounds inhibited both enzymes, and the selective inhibition of MAO-B by the major kiwifruit specialized metabolite D-(−)-quinic acid was observed. These results suggest that fruits and vegetables contain metabolites that inhibit the activity of MAO-A and -B, offering a potential natural option for the treatment of neurological disorders, in which MAOs are involved.

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“…Pure IF at 1 µM and 10 µM concentrations also showed remarkable effects on the lengths of axons and dendrites following a 4-day treatment [137,138]. Also, the monoamine oxidase B as the enzyme degeneration of nerve cells, involved in some neurological diseases including Alzheimer's disease was inhibited by pure IF, isolated from Acanthopanax senticosus, with a binding degree of 7.41% that had been determined by UPLC/MS/MS [30]. Considering these neuroprotective effects of IF, conducting more extensive studies on other aspects of this effect and involved pharmacological mechanisms could be helpful in introducing IF as a new drug molecule in the treatment of neurological diseases.…”

Section: Neuroprotective Effectsmentioning

confidence: 92%

“…Besides, the appearance of pieces at 194,166, 151(166-CH 3 ), and 123 m / z have been related to fracturing the C-O groups [ 38 , 70 , 100 ]. Also, another study reported that the IF showed a fragment at 163 m / z in MS/MS spectrum [ 30 ] ( Figure 3 ).…”

Section: Isofraxidinmentioning

confidence: 99%

“…IF and related compounds attenuate multiple destructive signaling mediators and therapeutic targets in some diseases [ 19 , 20 , 21 , 22 , 23 , 24 ]. In this regard, IF has shown several biological and pharmacological activities including antioxidant [ 25 ], cardioprotective [ 26 ], weight loss [ 27 ], anti-osteoarthritis [ 28 ], antimalarial [ 29 ], and neuroprotective [ 30 ] effects, with promising anticancer and anti-inflammatory effects. Accordingly, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), toll-like receptors (TLRs), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), inhibitory kappa B (IκBα), mitogen-activated protein kinases (MAPKs), and c-Jun N -terminal kinase (c-JNK) are the most targeted pathway for anticancer and anti-inflammatory effects of IF [ 27 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Isofraxidin: Synthesis, Biosynthesis, Isolation, Pharmacokinetic and Pharmacological Properties

et al. 2020

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Isofraxidin (7-hydroxy-6, 8-dimethoxy coumarin) (IF) is a hydroxy coumarin with several biological and pharmacological activities. The plant kingdom is of the most prominent sources of IF, which, among them, Eleutherococcus and Fraxinus are the well-known genera in which IF could be isolated/extracted from their species. Considering the complex pathophysiological mechanisms behind some diseases (e.g., cancer, neurodegenerative diseases, and heart diseases), introducing IF as a potent multi-target agent, which possesses several herbal sources and the multiple methods for isolation/purification/synthesis, along with the unique pharmacokinetic profile and low levels of side effects, could be of great importance. Accordingly, a comprehensive review was done without time limitations until February 2020. IF extraction methods include microwave, mechanochemical, and ultrasound, along with other conventional methods in the presence of semi-polar solvents such as ethyl acetate (EtOAc). In addition to the isolation methods, related synthesis protocols of IF is also of great importance. From the synthesis point of view, benzaldehyde derivatives are widely used as precursors for IF synthesis. Along with the methods of isolation and biosynthesis, IF pharmacokinetic studies showed hopeful in vivo results of its rapid absorption after oral uses, leading to different pharmacological effects. In this regard, IF targets varieties of inflammatory mediators including nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), tumor necrosis factor-α (TNF-α), and matrix metalloproteinases (MMPs). thereby indicating anticancer, cardioprotective, and neuroprotective effects. This is the first review on the synthesis, biosynthesis, isolation, and pharmacokinetic and pharmacological properties of IF in combating different diseases.

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Chemical characterization of small‐molecule inhibitors of monoamine oxidase B synthesized from the Acanthopanax senticosus root with affinity ultrafiltration mass spectrometry

Cited by 18 publications

References 30 publications

Different Metabolites in the Roots, Seeds, and Leaves of Acanthopanax senticosus and Their Role in Alleviating Oxidative Stress

Different Metabolites in the Roots, Seeds, and Leaves of Acanthopanax senticosus and Their Role in Alleviating Oxidative Stress

Inhibition of Human Monoamine Oxidases A and B by Specialized Metabolites Present in Fresh Common Fruits and Vegetables

Isofraxidin: Synthesis, Biosynthesis, Isolation, Pharmacokinetic and Pharmacological Properties

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