Chemical composition and antibacterial activity of essential oil of a Croton rhamnifolioides leaves Pax & Hoffm

Abstract

This study describes the chemical composition and antimicrobial activity of an essential oil from the leaves of Croton rhamnifolioides Pax&Hoffm., an aromatic plant widely distributed in northeastern Brazil and commonly used in folk medicine. Eight components were identified: 1.8-cineole (46.32%) as the major compound, followed by 1-felandreno (16.70%), p-cymene (10.21%), sabinene (8.14%) and trans-caryophyllene (4.81%). The essential oil exhibited antibacterial activity against Aeromonas hydrophila, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis and Staphylococcus aureus, with minimum inhibitory concentrations and minimum bactericidal concentrations ranging from 2.5 to 20 and 5 to 40 µL.mL -1 , respectively. In addition, the essential oil also affected the cellular viability of these bacteria. The results suggest the need for research to establish the potential uses of this essential oil as a natural antimicrobial in the food industry. Key words: Croton rhamnifolioides, essential oil, antimicrobial activity, chemical composition ResumoO presente estudo descreve a composição química e a atividade antimicrobiana do óleo essencial das folhas de Croton rhamnifolioides Pax & Hoffm, uma planta aromática amplamente distribuída no Nordeste do Brasil e largamente utilizada na medicina popular. Cromatografia gasosa -espectrometria de massa foi utilizada para determinar sua composição. Oito componentes foram identificados, sendo 1,8-cineol (46,32%) o composto majoritário, seguido por 1-felandreno (16,70%), p-cimeno (10,21%), sabineno (8,14%) e trans-cariofileno (4,81%). O óleo essencial apresentou atividade antibacteriana contra Aeromonas hydrophila, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis e Staphylococcus aureus com valores de concentração inibitória mínima (CIM) e concentração bactericida mínima (CBM) entre 2,5 -20 μL/mL e 5 -40 μL/mL, respectivamente. Além disso, o óleo essencial também afetou a viabilidade celular dessas bactérias. Os resultados sugerem a necessidade de estudos para estabelecer o uso potencial deste óleo essencial como antimicrobiano natural na indústria de alimentos. Palavras-chave: Croton rhamnifolioides, óleo essencial, atividade antimicrobiana, composição química

Chemical composition, antibacterial and modulatory action of the essential oil of Croton rhamnifolioides leaves Pax and Hoffman

“…The chemical composition of the essential oil of Croton rhamnifolioides leaves is similar to the compounds identified in the same species by COSTA et al, (2013), presenting four constituent in common, identified by the present study: α pinene, sabinene, linalol and germacrene-D. However, in the study by this author the 1,8-cineol was identified as the major constituent.…”

“…In studies undertaken by COSTA et al (2013), bacterial activity of the essential oil of Croton rhamnifolioides leaves was revealed against several bacteria, amongst them S. aureus and E. Coli presenting a MIC of 5 -1 µL/mL and 20 -1 µL/mL respectively via the gas contact method. This result was attributed to the presence of 1,8-cineol, the major constituent.…”

Essential Oil of Croton ceanothifolius Baill. Potentiates the Effect of Antibiotics against Multiresistant Bacteria

“…The genus Croton stands out for presenting a large number of species in tropical and subtropical regions [5] and for its extensive use in traditional medicine in communities of Asia, Africa, and South America [6]. In Brazil, Croton species have been used mainly to treat inflammatory [7] and infectious [8,9] diseases. The popular use of this genus led to the development of scientific research to validate its actions.…”

Circadian Rhythm and Larvicidal Activity Against Aedes aegypti of Essential Oils from Croton piauhiensis

“…16 Furthermore, several studies have reported the chemical characterization and biological activities of EOs extracted from Croton species. [17][18][19][20][21] In recent years, the search for efficient natural compounds with larvicidal activity against A. aegypti and low environmental toxicity has increased, thus EOs of Croton species are outstanding candidates, since they are in some cases, they are readily available, and economically viable. In fact, the larvicidal activity of EOs extracted from C. zehntneri, C. jacobinensis, C. argyrophylloides, C. sonderianus, C. nepetaefolius and C regelianus have been previously reported.…”