Chemical composition and antidermatophytic properties of volatile fractions of hexanic extract from leaves of Cupressus lusitanica Mill. from Cameroon

Kuiaté, Jules-Roger; Bessière, J.; Zollo, Paul Henri Amvam; Kuate, Serge Philibert

doi:10.1016/j.jep.2005.07.022

Cited by 43 publications

(25 citation statements)

References 9 publications

(9 reference statements)

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“…Previous studies have shown that 30 % of the marketed drugs contain active principles that were isolated for the first time from plants [1]. Herbal preparations prepared from medicinal plants can bring about a solution to certain disorders, mostly in developing countries where the commercial drugs are expensive and not easily available to the poor people [2]. In fact, there is lack of evidence on quality, safety and efficacy of many herbal preparations.…”

Section: Introductionmentioning

confidence: 99%

Acute and Subchronic Oral Toxicity Assessment of the Ethanolic Extract of the root of <i>Oncoba spinosa</i> (Flacourtiaceae) in Rodents

Prasanth¹,

Suba²,

Bommireddy³

et al. 2015

Trop. J. Pharm Res

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Section: Introductionmentioning

confidence: 99%

Acute and Subchronic Oral Toxicity Assessment of the Ethanolic Extract of the root of <i>Oncoba spinosa</i> (Flacourtiaceae) in Rodents

Prasanth¹,

Suba²,

Bommireddy³

et al. 2015

Trop. J. Pharm Res

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“…Kaurenoic acid (entkaur-16-en-19-oic acid) and its derivatives are potent uterine muscle relaxants, through ß 2 adrenergic receptor independent mechanisms, probably antagonist of the calcium channel (Tirapelli et al, 2004). In vitro, PMN and lymphocyte proliferative activities are reported (Ohkoshi et al, 2004), as well as antimicrobial and antifungal activity (Yatsuda et al, 2005, Kuiate et al, 2006, and non-specifi c cytotoxicity (Costa-Lotufo et al, 2002). Kaurenoic acid is considered a universal marker of Copaiba oil, but could not be found in every harvest .…”

Section: Introductionmentioning

confidence: 99%

Isolation of syringaldehyde from Mikania laevigata medicinal extract and its influence on the fatty acid profile of mice

Pedroso¹,

Santos²,

Steil³

et al. 2008

Rev. bras. farmacogn.

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RESUMO: "Extrato medicinal de Mikania laevigata: infl uência no perfi l de ácidos graxos em camundongos e isolamento de siringaldeído por CLAE semipreparativa". O gênero Mikania é popularmente conhecido como "guaco" e é utilizado para tratar febre, reumatismo, resfriados e afecções respiratórias. Em trabalho prévio demonstramos sinergismo entre os componentes do extrato de M. laevigata para produzir os efeitos farmacológicos esperados, incluindo a cumarina e seu precursor ácido o-cumárico como marcadores. Muitas espécies de Mikania são produtoras de diterpenos ent-caurenos que apresentam atividade antispasmódica e relaxante da musculatura lisa. Buscando a padronização do extrato medicinal de guaco (preparado segundo a farmacopéia brasileira 1ª edição), este trabalho visou determinar a presença de ácido caurenóico através de CLAE-DAD e isolou siringaldeído através de CLAE semipreparativa, sendo que o primeiro não foi encontrado no extrato e o siringaldeído é um dos seus componentes majoritários. Camundongos isogênicos Balb-C portadores de pneumonite alérgica foram tratados com este extrato, e amostras de pulmão e fígado foram analisadas por CG-DIC quanto ao seu conteúdo de ácidos graxos. A quantidade de ácido araquidônico (ARA) e de ácido docosahexaenóico (DHA) encontrada demonstrou que a composição é distinta em ambos tecidos, e apenas a concentração de DHA hepático foi alterado em função do tratamento, o qual não foi encontrado no pulmão. Não foi detectada diferença signifi cativa na produção de ARA. Tanto o extrato aquoso, quanto a cumarina e o ácido o-cumárico, estimularam a síntese de DHA no fígado (p < 0.05). Unitermos:Mikania laevigata, Asteraceae, ácido caurenóico, siringaldeído, ácido araquidônico, ácido docosahexaenóico. ABSTRACT:The Mikania genus is widely known as "guaco" and is used to treat fever, rheumatism, fl u and respiratory diseases. Our previous work evidenced the synergism among M. laevigata extract components to produce desirable effects, and included the coumarin precursor, o-coumaric acid as marker. Many Mikania species are producers of ent-kaurene diterpenes which presented antiespasmodic and relaxant activities on smooth muscle. Seeking to standardize the guaco extract, which is registered in the Brazilian Pharmacopoea, this paper deals with the determination of kaurenoic acid through LC-PDA and the isolation through LC of syringaldehyde. Kaurenoic acid was not found in the extract, and syringaldehyde is one of the major compounds of pharmacopoeal extract, together with coumarin and o-coumaric acid. Samples from the lung and liver of Balb-C isogenic allergic pneumonitis bearing mice, treated with the same extract, were analyzed through GC-FID, and the fatty acid content was determined and analyzed. The results obtained by measuring the arachidonic acid (ARA) and docosahexaenoic acid (DHA) in the liver and lung of treated animals demonstrated that the fatty acid composition is distinct in both tissues, and that in the liver, only the DHA was altered as a result of the treatments. DHA is ...

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“…Individual activities of these compounds have been demonstrated [29]. Also, from our previous work, the antidermatophytic activities of triterpenes [30], essential oil of Ageratum houstonianum [31] and the volatile fraction of Cupressus lusitanica , constituted of monoterpens, sesquiterpens and triterpens [32], were reported. The n-hexane fraction was almost inactive, indicating that the active principle may not belong to the family of alkaloid.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo antidermatophytic activity of the dichloromethane-methanol (1:1 v/v) extract from the stem bark of Polyscias fulva Hiern (Araliaceae)

Njateng

Gatsing

Mouokeu

et al. 2013

BMC Complement Altern Med

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BackgroundDuring the last decades, the number of people suffering from dermatophytoses has seriously increased, mainly due to the development of resistant strains of microorganisms to a range of formally efficient antibiotics. Polyscias fulva, a medium size tree which grows in the West Region of Cameroon is traditionally used for local application against dermatoses and orally against venereal infections. The dichloromethane-methanol (1:1 v/v) extract from the stem bark of Polyscias fulva was evaluated for its in vitro and in vivo antifungal activities.MethodsThe plant extract was prepared by maceration of its stem bark powder in CH2Cl2-MeOH (1:1 v/v). The extract obtained was successively partitioned in hexane, ethyl acetate and n-butanol. Phytochemical screening was performed using standard methods. In vitro antidermatophytic activity was assayed by the well diffusion and broth microdilution methods. The degree of dermal irritation of the crude extract was determined in guinea pigs using the occluded dermal irritation test method. The in vivo antidermatophytic activity of the extract-oil formulation (1.25, 2.5 and 5% w/w concentrations) was evaluated using Trichophyton mentagrophytes-induced dermatophytosis in a guinea pigs model.ResultsPhytochemical screening indicated that, the crude extract, ethyl acetate, n-butanol and residue fractions contain in general saponins, tannins, alkaloids, anthraquinones and phenols while the hexane fraction contains only alkaloids. The ethyl-acetate, n-butanol and residue fractions displayed higher antifungal activities (MIC = 0.125-0.5 mg.mL-1) against eight dermatophytes as compared to the crude extract (MIC = 0.5-1 mg.mL-1). This latter appeared to have slight perceptible erythema effects on guinea pigs as the primary irritation index (PII) was calculated to be 0.54. In vivo, the antidermatophytic activities of the extract-oil formulations were dose-dependent. Griseofulvin-oil 5% at 0.01 g/kg and formulated extract-oil (5%) at 0.1 g/kg eradicated the microbial infection after thirteen and fourteen days of daily treatment respectively.ConclusionsThe results of preclinical in vitro and in vivo evaluations indicate that the extract-oil formulation at 5% may constitute an alternative means to alleviate fungal infections caused by dermatophytes.

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Chemical composition and antidermatophytic properties of volatile fractions of hexanic extract from leaves of Cupressus lusitanica Mill. from Cameroon

Cited by 43 publications

References 9 publications

Acute and Subchronic Oral Toxicity Assessment of the Ethanolic Extract of the root of <i>Oncoba spinosa</i> (Flacourtiaceae) in Rodents

Acute and Subchronic Oral Toxicity Assessment of the Ethanolic Extract of the root of <i>Oncoba spinosa</i> (Flacourtiaceae) in Rodents

Isolation of syringaldehyde from Mikania laevigata medicinal extract and its influence on the fatty acid profile of mice

In vitro and in vivo antidermatophytic activity of the dichloromethane-methanol (1:1 v/v) extract from the stem bark of Polyscias fulva Hiern (Araliaceae)

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