Chemical composition and antioxidant potential of Cannabis sativa L. roots

Kornpointner, Christoph; Martinez, Aitor Sainz; Marinovic, Silvija; Haselmair-Gosch, Christian; Jamnik, Polona; Schröder, Katharina; Löfke, Christian; Halbwirth, Heidi

doi:10.1016/j.indcrop.2021.113422

Cited by 56 publications

(43 citation statements)

References 38 publications

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“…The substances identified in the C. sativa root extracts normally have ethanol, methanol or hexane as the solvent, with few reports of the chemical composition of aqueous extracts. In general, the most commonly found substances in C. sativa roots are friedelin, epifriedelanol, cannabisativine, anhydrocannabisativine, β-sitosterol, stigmasterol, β-amyrin, and campesterol [21][22][23] Transient receptor potential vanilloid type 1 UNIVASF Universidade Federal do Vale do São Francisco Δ 9 -THC Δ 9 -tetrahydrocannabinol not been able to identify some of the substances already identified and there was a variation on the relative percentage of such compounds depending on the sample. The variability in the synthesis of secondary metabolites on plants can occur due to several defense mechanisms against the stresses produced by the environment, but this variation can be observed at all scales, including populations, individuals, and different parts of the plant, indicating that the chemical complexity within a population is related more to the individual than collective factors.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Chemical Analysis by LC-MS of Cannabis sativa Root Samples from Northeast Brazil and Evaluation of Antitussive and Expectorant Activities

et al. 2021

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Cannabis sativa is a millenary medicinal plant. However, contrary to worldwide paradigm-shifting, countries like Brazil still prohibit C. sativa cultivation and its medicinal use, even though many populations use aerial parts and roots of this plant for healthcare. As such, the objective of this work was to identify substances in the samples of the C. sativa roots, tracing a correlation with antitussive and expectorant effects. Therefore, samples of C. sativa roots were donated by the Polícia Federal Brasileira, and its aqueous extract (AECsR) was prepared with subsequent lyophilization, to maintain the material stability. After that, the material was analyzed by LC-MS to observe its chemical profile. Four samples (AECsR-A, B, C, and D) were tested in animal models of citric acid-induced cough (0.4 M) and phenol red expectoration (500 mg/kg). Using LC-MS it was possible to identify 5 molecules in C. sativa roots: p-coumaroyltyramine, tetrahydrocannabinol-C4, feruoiltyramine, anhydrocanabisativine, and cannabisativine. In experimental protocols, male mice (Mus musculus) were treated with samples of AECsR at doses of 12.5, 25, or 50 mg/kg regardless of the pharmacological test. In these tests, all samples showed the potential to treat cough and promote fluid expectoration, differing only in the dose at which these effects were observed. Therefore, the data showed that the C. sativa roots of the Brazilian Northeast showed antitussive and expectorant effects, even with intense secondary metabolitesʼ variation, which alters its potency, but not its effect. This highlights the importance of this medicinal plant for future therapy and corroborates to traditional use.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Chemical Analysis by LC-MS of Cannabis sativa Root Samples from Northeast Brazil and Evaluation of Antitussive and Expectorant Activities

et al. 2021

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“…It is a plant species belonging to family, Cannabaceae and genus Cannabis, alongside two other species, C. indica , and C. ruderalis . Aside from the proteins, different parts of industrial hemp are rich in bioactive compounds, including the two major constituents of the aerial parts such as cannabinoids (especially cannabidiol and Δ 9 ‐tetrahydrocannabinol), and other constituents such as polyphenols, phospholipids, sulfolipids, proanthocyanidins, terpenes, and phytosterols present in the roots and seeds (Antonelli et al, 2020; Kornpointner et al, 2021; Radwan et al, 2021). The inflorescence extract has been shown to prolong the shelf‐life of vegetable oil by delaying their oxidative rancidity through inhibition of unsaturated fatty acid peroxidation (Cantele et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A review on the techno‐functional, biological, and health‐promoting properties of hempseed‐derived proteins and peptides

Aguchem

Okagu

et al. 2022

Journal of Food Biochemistry

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Protein-energy malnutrition is a global challenge that demands urgent attention, especially with the increasing population growth and unmatched food security plans.One strategy is to expand the list of protein sources, such as neglected and underutilized crops, with high protein content. A good number of plant proteins, in addition to their nutritional benefits, exert therapeutic properties as seen in seeds derived from legumes and emerging sources such as hemp. In this review, the transepithelial transport, functional, and biological properties of hempseed proteins (HSPs) and peptides were discussed. The review also described the potential safety issues of incorporating hempseeds in food products. Due to the multitargeted effects of hempseed-derived proteins and their peptides against many chronic diseases, and their functional properties, current knowledge shows that hempseed has tremendous potential for functional food and nutraceutical applications. | FUN C TIONAL PROPERTIE S OF HEMPS EED PROTEINSBeside the nutritional properties of HSPs resulting from its highly digestible and well-balanced essential amino acid composition, which is in strong agreement with FAO/WHO requirements for infants and children (Tang et al., 2006;Teh & Birch, 2013), and physicochemical properties such as hydrophobicity and polypeptide composition, it has also demonstrated techno-functional properties. These properties include water solubility, emulsifying, foaming, gelation,

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“…Hemp (Cannabis sativa L.) plant is a multipurpose industrial crop widely cultivated in the world (1). In recent years, the application of various parts of hemp plant, such as hemp inflorescence, hempseed oil, and hemp sprouts, has represented a valuable source of nutraceuticals and functional foods (2,3).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical differences of hemp (Cannabis sativa L.) leaves from different germplasms and their regulatory effects on lipopolysaccharide-induced inflammation in Matin-Darby canine kidney cell lines

et al. 2022

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The increasing demand of hemp (Cannabis sativa L.) has attracted more interest in exploring its phytochemical profile and bioactivities, such as anti-inflammatory effect. In this study, the phytochemicals of different hemp leaves were investigated, with the content order: total saponins content (TSC) > total alkaloids content (TAC) > total phenolics content (TPC) > total flavonoids content (TFC) > cannabinoids. Hemp leaves from Shanxi accumulated higher flavonoids and cannabinoids (i.e., THC, CBD, and CBN), while phenolics were more abundant in those from Hunan. A lipopolysaccharide (LPS)-induced inflammatory Matin-Darby canine kidney (MDCK) cell model was established to evaluate the anti-inflammatory effects of hemp leaf extracts. Hemp leaf extracts, especially the D129 and c7, significantly increased cell viability of LPS-induced inflammatory MDCK cells, and D132 significantly decreased the secretion of pro-inflammatory cytokines (TNF-α and IL-6) and the lactate dehydrogenase (LDH) activity. Except for c12, other hemp leaf extracts obviously decreased the cell morphological damage of LPS-induced inflammatory MDCK cells. The correlation analysis revealed that cannabinol (CBN) and TPC showed the strongest correlation with anti-inflammatory activities, and hierarchical clustering analysis also showed that hemp germplasms from Shanxi might be good alternatives to the common cultivar Ym7 due to their better anti-inflammatory activities. These results indicated that hemp leaves were effective in LPS-induced inflammatory MDCK cells, and flavonoids and cannabinoids were potential geographical markers for distinguishing them, which can provide new insights into the anti-inflammatory effect of hemp leaves and facilitate the application of hemp leaves as functional ingredients against inflammatory-related disorders.

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Chemical composition and antioxidant potential of Cannabis sativa L. roots

Cited by 56 publications

References 38 publications

Chemical Analysis by LC-MS of Cannabis sativa Root Samples from Northeast Brazil and Evaluation of Antitussive and Expectorant Activities

Chemical Analysis by LC-MS of Cannabis sativa Root Samples from Northeast Brazil and Evaluation of Antitussive and Expectorant Activities

A review on the techno‐functional, biological, and health‐promoting properties of hempseed‐derived proteins and peptides

Phytochemical differences of hemp (Cannabis sativa L.) leaves from different germplasms and their regulatory effects on lipopolysaccharide-induced inflammation in Matin-Darby canine kidney cell lines

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