Chemical Composition and Antioxidant Properties of Common and Lemon Verbena

Połumackanycz, Milena; Petropoulos, Spyridon Α.; Añibarro-Ortega, Mikel; Pinela, José; Barros, Lillian; Plenis, Alina; Viapiana, Agnieszka

doi:10.3390/antiox11112247

Cited by 3 publications

(4 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Verbenaceae family includes approximately 2000 species and more than 100 genera, grown over a broad geographic range that covers tropical, subtropical, and temperate regions across the globe [16]. Since ancient times, aromatic species in this family have been utilized as flavorings in foods and beverages [17].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant-Rich Extracts from Lemon Verbena (Aloysia citrodora L.) Leaves through Response Surface Methodology

Athanasiadis,

Chatzimitakos,

Makrygiannis

et al. 2024

Oxygen

View full text Add to dashboard Cite

A member of the Verbenaceae family, Aloysia citrodora, or lemon verbena, is a medicinal herb with antioxidant compounds. The aim of this study was to develop a green, optimized method for the bioactive compound (carotenoids, ascorbic acid, and polyphenols) extraction from lemon verbena leaves through response surface methodology (RSM). The bioactive compound recovery was shown to be significantly affected by the extraction technique (both with pulsed electric field and ultrasound-assisted extraction), along with an extraction solvent, based on partial least squares analysis. Consequently, the maximum polyphenol yield required a double-assisted extraction with a relatively low extraction duration (60 min) at a high temperature (80 °C), with a moderate-polarity extraction solvent (50% v/v ethanol). With the optimized method, the total polyphenol content (TPC) was measured at 175.03 mg gallic acid equivalents/g, whereas chromatographic analysis revealed that verbascoside was the most prevalent polyphenol (132.61 mg/g). The optimum extract provided a high antioxidant capacity through the measurements of FRAP (1462.17 μmol ascorbic acid equivalents (AAE)/g), DPPH (1108.91 μmol AAE/g), and H2O2 (1662.93 μmol AAE/g). Total carotenoids were measured at 499.61 μg/g, with ascorbic acid at 8.36 μg/g. Correlation analyses revealed a negative correlation of the latter compound with color coordinates. This study highlights the potential of lemon verbena leaves to be used in pharmaceutical and food industries.

show abstract

Section: Introductionmentioning

confidence: 99%

Antioxidant-Rich Extracts from Lemon Verbena (Aloysia citrodora L.) Leaves through Response Surface Methodology

Athanasiadis,

Chatzimitakos,

Makrygiannis

et al. 2024

Oxygen

View full text Add to dashboard Cite

show abstract

“…Trolox equivalence antioxidant capacity (TEAC) based on the ability of antioxidants to scavenge 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS • ) radical [18][19][20][21][22][23];…”

Section: Introductionmentioning

confidence: 99%

“…Antioxidant capacity based on the reaction of antioxidants with 2,2-diphenyl-1picrylhydrazyl (DPPH • ) [18][19][20][21][22][23][24];…”

Section: Introductionmentioning

confidence: 99%

“…Total phenolic contents by reaction with Folin-Ciocalteu reagent based on the reduction of molybdotungsto-phosphoric heteropolyanion to molybdenum-tungsten blue [18,20,23,24,27,30]; • Ferric-reducing antioxidant power (FRAP) based on the reduction of 2,4,6-tripyridyl-S-triazine complex of Fe 3+ in reaction with the antioxidants [18,19,22,23,32]; • Cupric reducing antioxidant capacity (CUPRAC) based on the reduction of the Cu(II)neocuproine complex by antioxidants [23,[33][34][35].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Characterization of the Antioxidant Properties of Medicinal Plants and Products: A Review

Ziyatdinova

Kalmykova

2023

Molecules

View full text Add to dashboard Cite

Medicinal plants are an important source of bioactive compounds with a wide spectrum of practically useful properties. Various types of antioxidants synthesized in plants are the reasons for their application in medicine, phytotherapy, and aromatherapy. Therefore, reliable, simple, cost-effective, eco-friendly, and rapid methods for the evaluation of antioxidant properties of medicinal plants and products on their basis are required. Electrochemical methods based on electron transfer reactions are promising tools to solve this problem. Total antioxidant parameters and individual antioxidant quantification can be achieved using suitable electrochemical techniques. The analytical capabilities of constant-current coulometry, potentiometry, various types of voltammetry, and chrono methods in the evaluation of total antioxidant parameters of medicinal plants and plant-derived products are presented. The advantages and limitations of methods in comparison to each other and traditional spectroscopic methods are discussed. The possibility to use electrochemical detection of the antioxidants via reactions with oxidants or radicals (N- and O-centered) in solution, with stable radicals immobilized on the electrode surface, via oxidation of antioxidants on a suitable electrode, allows the study of various mechanisms of antioxidant actions occurring in living systems. Attention is also paid to the individual or simultaneous electrochemical determination of antioxidants in medicinal plants using chemically modified electrodes.

show abstract

LC–ESI–MS/MS-based molecular networking, antioxidant, anti-glioma activity and molecular docking studies of Clematis graveolens

Ahmed,

Ikram,

Khan

et al. 2024

Plant Methods

View full text Add to dashboard Cite

Clematis graveolens Lindl., an indigenous climbing plant found in the Himalayan areas, is used by local communities for the treatment of neck tumors. The objective of this work is to examine the comprehensive metabolomic profile, antioxidant capability, in vitro and in silico anti-glioma effects on U-87 human glioma cell lines of the crude extract and fractions from C. graveolens. Liquid chromatography coupled with mass spectroscopy (LC–MS/MS) was used to establish detailed metabolite profiling of C. graveolens. The assessment of cell cytotoxicity was conducted using MTT cell viability assay on U-87 and BHK-21. Through molecular docking studies, the mode of inhibition and binding interaction between identified compounds and target proteins were also determined to evaluate the in vitro results. The use of LC–MS/MS-based global natural products social (GNPS) molecular networking analysis resulted in the identification of 27 compounds. The crude extract, ethyl acetate fraction, and chloroform fraction exhibited significant inhibitory activity against the U-87 cell lines, with IC50 values of 112.0, 138.1, and 142.7 µg/mL, respectively. The ethyl acetate fraction exhibited significant inhibitory concentration for 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity and the metal chelation activity with IC50 value of 39.50 µg/mL, 32.27 µg/mL, and 53.46 µg/mL, respectively. The crude extract showed maximum total phenolic, and total flavonoid concentration measuring 338.7 µg GAE/mg, and 177.04 µg QE/mg, respectively. The findings of this study indicate that C. graveolens consists of a diverse range of active phytoconstituents that possess antioxidant and anti-glioma properties.

show abstract

Chemical Composition and Antioxidant Properties of Common and Lemon Verbena

Cited by 3 publications

References 74 publications

Antioxidant-Rich Extracts from Lemon Verbena (Aloysia citrodora L.) Leaves through Response Surface Methodology

Antioxidant-Rich Extracts from Lemon Verbena (Aloysia citrodora L.) Leaves through Response Surface Methodology

Electrochemical Characterization of the Antioxidant Properties of Medicinal Plants and Products: A Review

LC–ESI–MS/MS-based molecular networking, antioxidant, anti-glioma activity and molecular docking studies of Clematis graveolens

Contact Info

Product

Resources

About