Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts

Abstract

Phytolacca, which belongs to the family of Phytolaccaceae, are known for their use in popular medicine. Bioactivity of five extracts from Phytolacca dioica seeds were evaluated in four bioassays. A selected group of compounds from the extract that displayed the best bioactivity was analysed. The ethyl acetate extract (EAE) possessed the highest content of phenolics, the highest inhibitory activity on the tyrosinase and xanthine oxidase enzymes and showed a high antioxidant activity. HPLC-DAD-MS was employed to identify the phenolics profile of the most active one (EAE). HSCCC analysis of the EAE led to the isolation of phytolaccoside B and a mixture of 4 isomers, isoamericanol B1, B2, C1 and C2. These isoamericanol isomers presented activity against tyrosinase and xanthine oxidase. Our results revealed for the first time an interesting biological activity of the extract and isolated compounds from P. dioica seeds, which could be considered as a source of bioactive molecules.

Insecticidal Activities Against Odontotermes formosanus and Plutella xylostella and Corresponding Constituents of Tung Meal from Vernicia fordii

“…The fragment ion of m/z 165 was similar to americanol A and isoamericanol A. By comparing the fragmentation pathways with those of the references, compounds 6, 10, 13, 14, 15, and 16 could be tentatively identified as isoamericanol B1, isoamericanol B2, isoamericanol C1, isoamericanol C2, princepin, and isoprincepin [ 24 ], which was in accord with the findings of Chen et al [ 23 ]. Those compounds were also named sesquineolignans because they were structurally formed by the oxidative polymerization of three phenylpropyl.…”

Anti-Aging Potential of Extracts from Washingtonia filifera Seeds

“…The inhibition of tyrosinase activity by W. filifera extracts was determined by using 3,4-dihydroxyphenylalanine (L-DOPA) as a substrate [ 20 ]. The reaction mixture contained 25 mM phosphate buffer (pH 6.8), mushroom tyrosinase (100 U/mL, final concentration), with or without a plant extract solution.…”

Screening and analysis of xanthine oxidase inhibitors in jute leaves and their protective effects against hydrogen peroxide-induced oxidative stress in cells

“…Also, these inhibitors show more more side effects [9][10][11]. On the basis of these results, novel xanthine oxidase inhibitors with fewer side effects should be explored in natural products for better effects [12,13].…”