Chemical Composition and Immunobiological Activities of Sodium Dodecyl Sulphate Extracts from the Cell Envelopes of Actinobacillus Actinomycetemcomitans, Bacteroides Gingivalis and Fusobacterium Nucleatum

Kato, Kimiko; Kokeguchi, Susumu; Ishihara, Hiroko; Murayama, Yoji; Tsujimoto, M; Takada, Haruhiko; Ogawa, Tomohiko; Kotani, Shozo

doi:10.1099/00221287-133-4-1033

Cited by 15 publications

(11 citation statements)

References 7 publications

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“…High molecular weight (120 to 130 kD) OMPs were also observed in these B. intermedius OMs. B. gingivalis strains 2022, 2013, W, and ATCC 33277, in the studies of Williams & Holt (49) or those of Kato et al (18), who employed sonication and SDS extraction did not show the coneentration of low molecular weight proteins which were observed in this study when the OMs were prepared by French pressure disruption. Boyd & MeBride (4) also isolated an OMF from B. gingivalis W12 by tnanual shearing.…”

Section: Discussioncontrasting

confidence: 43%

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Comparative studies of the outer membranes of Bacteroides gingivalis, strains ATCC 33277, W50, W83,381

Kennell

Holt

1990

Oral Microbiology and Immunology

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Outer membrane fractions from Bacteroides gingivalis strains ATCC 33277, 381, W50, and W83 were isolated by French pressure cell disruption and the distribution of major and minor proteins was determined by SDS-PAGE electrophoresis after treatment with 2% Sarkosyl and 2% Triton X-100. Heat-modifiability of the outer membrane proteins (OMPs) from these B. gingivalis strains was also determined after treatment at 100 degrees C and analysis by both 1- and 2-dimensional SDS-PAGE. The distribution of the OMPs on the surface of these B. gingivalis strains was determined by 125I labelling. For the most part of the OMPs of B. gingivalis presented a complex distribution, with OMPs observed between 123 kD and 13 kD. While the distribution of the OMPs was strain specific, OMPs common to all of the strains were observed. Two percent Sarkosyl treatment of the OMs at room temperature resulted in the solubilization of approximately 60% of the OMP. The Sarkosyl-insoluble MOMPs had relative molecular weights between 110 kD and 20 kD. Many of the OMPs which were separated at room temperature were heat-modified at either 65 degrees C or 100 degrees C. Heating of the OMs at 100 degrees C resulted in the heat modification of the majority of those OMPs observed at room temperature. Sarkosyl-100 degrees C OMs displayed MOMPs at apparent molecular weights between 90 kD and 15 kD. Radioiodination of the B. gingivalis strains ATCC 33277, 381, W83 and W50 revealed between 7 and 14 MOMPs at the cell surface depending upon the strain. The complexity of the OM of these B. gingivalis strains indicated the possibility of identifying and separating those OMPs involved in a variety of biological functions, including virulence, transport, and cell interaction.

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Section: Discussioncontrasting

confidence: 43%

“…When heated at 65°C (Fig. 2, Lane 2), the strain W50 OMF resolved its MOMPs into 9 bands with relative molecular weights of 46,42,37,32,29,24,20,18 Identical to B. gittgivalis strain W83, strain W50 OMs exposed to 2% Sarkosyl, 2% Triton X-100 and 2% Triton X-100-1-MgCl, (Fig. 2, Lanes 4^6, 8-10, 11-13) resolved the detergent insoluble MOMPs.…”

Section: Resultsmentioning

confidence: 98%

Comparative studies of the outer membranes of Bacteroides gingivalis, strains ATCC 33277, W50, W83,381

Kennell

Holt

1990

Oral Microbiology and Immunology

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“…Major cell-surface proteins for A. actinomycetemcomitans have been described (DiRienzo and Spieler, 1983;Kato et al, 1987). Serum IgG antibody responses to outer membrane antigens from A. actinomycetemcomitans serotype c in normal, localized juvenile and generalized periodontitis subjects identified antigens that were detected more frequently in generalized disease (e.g., 40 and 70 kDa), antigens that were identified less frequently in localized disease (e.g., 29 kDa), and antigens that were detected by sera from all groups (e.g., 19, 24, 35, and 67 kDa) (Watanabe et al, 1989 antigen bands in OMA from A. actinomycetemcomitans Y4 stratified based upon the total IgG antibody reactivity in the human sera.…”

Section: Discussionmentioning

confidence: 99%

Antigen Specificity of Serum Antibody in A. actinomycetemcomitans-infected Periodontitis Patients

Ebersole¹,

Cappelli²,

Sandoval³

et al. 1995

J Dent Res

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We hypothesized that serum antibody with selected antigen specificities would relate to infection and disease in the patients and, thus, describe the characteristics of potential protective antibody. This study used serum samples from 24 periodontitis patients with subgingival infection and elevated serum IgG antibody to A. actinomycetemcomitans to define the antigenic specificities of IgG, IgM, IgA, and IgG1-4 antibody to A. actinomycetemcomitans strain Y4 outer membrane antigens (OMA). Uniform IgG antibody (> 70% of the patients) was noted to antigens with M(r) of 65, 38, 29, and 17 kDa. Both IgA and IgM specificities reflected those shown for IgG in each patient. IgG1 and IgG2 antibody reacted with several OMA bands in each patient, while IgG3 antibodies were directed to numerous OMA bands in many patients and represented the most broad-based response. The IgG4 response patterns were limited to a few OMA bands. We noted a prominent occurrence of IgG reactions with OMA bands that were characteristic for individual patients. The frequency of responses to OMA of higher M(r) (i.e., > 80 kDa) and to the 34-, 31-, and 24-kDa antigens was positively related to the total IgG antibody levels. Antibody reactive with OMA bands at 65-, 38-, 29-, 17-, 15-, and 11-kDa antigens was detected in patients with few to many teeth infected with A. actinomycetemcomitans. Furthermore, patients with a high percentage of teeth with > or = 6 mm pockets had a decreased frequency of responses to the high-M(r) antigens (i.e. > 90 kDa) as well as to the 58-kDa antigen.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…In order to estimate the direct cytotoxicity of periodontopathogenic bacteria toward HGF in vitro, several cytotoxic activities such as growth inhibition of HGF by bacterial cells and LPS, im-munological activities of cell envelope extract, and the effect on the synthesis of HGF components were detertnined (1,5,10,12,13,22,24). In this study, the cytotoxicity was determined by assessing the effect of the bacterial constituents on the morphological change and the viability of the HGF cells that had been cultured to a confiuent monolayer.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Porphyromonas gingivalis toward cultured human gingival fibroblasts

Morioka

Hinode

Nagata

et al. 1993

Oral Microbiology and Immunology

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Direct cytotoxicity of black-pigmented anaerobic rods was studied on the confluent monolayer of human gingival fibroblasts in vitro. Only strains of Porphyromonas gingivalis caused morphological alteration (cell-rounding) and notable depression of viability of fibroblasts. To determine the location of the cytotoxicity, bacterial surface components, i.e., outer membrane, lipopolysaccharide, fimbriae and outer membrane vesicles were prepared from P. gingivalis and their cytotoxicity was assessed. Among these preparations, only outer membrane vesicles are supposed to have high affinity to human gingival fibroblasts, and the cytotoxicity of outer membrane vesicles was found to be much stronger than that of the other constituents. This cytotoxic factor seemed to consist largely of protein and to be associated with the enzyme activity of outer membrane vesicles. The effects of some protease inhibitors and L-cysteine on the cytotoxicity of outer membrane vesicles suggest that the mechanism of cell-rounding is different from that of cell death.

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Chemical Composition and Immunobiological Activities of Sodium Dodecyl Sulphate Extracts from the Cell Envelopes of Actinobacillus Actinomycetemcomitans, Bacteroides Gingivalis and Fusobacterium Nucleatum

Cited by 15 publications

References 7 publications

Comparative studies of the outer membranes of Bacteroides gingivalis, strains ATCC 33277, W50, W83,381

Comparative studies of the outer membranes of Bacteroides gingivalis, strains ATCC 33277, W50, W83,381

Antigen Specificity of Serum Antibody in A. actinomycetemcomitans-infected Periodontitis Patients

Cytotoxicity of Porphyromonas gingivalis toward cultured human gingival fibroblasts

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