Chemical Composition of the Acrosomes of Ram Spermatozoa

Removal of Acrosomes of Ram and Rabbit Spermatozoa

“…The r\l=o^\leof acrosomal enzymes of mammalian spermatozoa in sperm penetration through the investments of the ovum and subsequent fertilization has been studied since the development of the method based on treatment of spermatozoa with the cationic detergent, Hyamine, for detachment of acrosomes (Hartree & Srivastava, 1965). Srivastava, Zaneveld & Williams (1970) modified this method, using Triton X-100 in combination with Hyamine to obtain the release of membrane-bound enzymes.…”

The activity of spermatozoa of Periplaneta

“…There is considerable disagreement concerning the presence and function of a hyaluronidase in the acrosome (Chang, 1959;Kurzrok and B:irnberg, 1958;Mann, 1964;Moghissi et al, 1964;and Hartree and Srivastava, 1965 When such a physiological change results in an increased ability of the spermatozoa to fertilise the eggs of the female, they are said to be capacitated.…”

Sialic Acid in Purified Human Sperm

“…Furthermore, sialic acid may play an important role in the process of fertilization [19]. Several authors reported the sialic acid content in human sperm [ 10,11,161. These authors used the washed sperm prepared by centrifugation of the diluted semen.…”