Chemical Compositions and Experimental and Computational Modeling of the Anticancer Effects of Cnidocyte Venoms of Jellyfish Cassiopea andromeda and Catostylus mosaicus on Human Adenocarcinoma A549 Cells

Abstract

Nowadays, major attention is being paid to curing different types of cancers and is focused on natural resources, including oceans and marine environments. Jellyfish are marine animals with the ability to utilize their venom in order to both feed and defend. Prior studies have displayed the anticancer capabilities of various jellyfish. Hence, we examined the anticancer features of the venom of Cassiopea andromeda and Catostylus mosaicus in an in vitro situation against the human pulmonary adenocarcinoma (A549) cancer cell line. The MTT assay demonstrated that both mentioned venoms have anti-tumoral ability in a dose-dependent manner. Western blot analysis proved that both venoms can increase some pro-apoptotic factors and reduce some anti-apoptotic molecules that lead to the inducing of apoptosis in A549 cells. GC/MS analysis demonstrated some compounds with biological effects, including anti-inflammatory, antioxidant and anti-cancer activities. Molecular docking and molecular dynamic showed the best position of each biologically active component on the different death receptors, which are involved in the process of apoptosis in A549 cells. Ultimately, this study has proven that both venoms of C. andromeda and C. mosaicus have the capability to suppress A549 cells in an in vitro condition and they might be utilized in order to design and develop brand new anticancer agents in the near future.

Antioxidant and anticholinesterase properties of Echinometra mathaei and Ophiocoma erinaceus venoms from the Persian Gulf

“…The isolated compounds were identified by comparing them with data from the National Institute of Standards and Technology Library (NIST MS database, 2014). The relative quantity (%) of each component was determined by comparing the average peak area with the total area ( Zare et al, 2023 ).…”

“…The total GC run time was 37 min. The isolated compounds were The relative quantity (%) of each component was determined by comparing the average peak area with the total area (Zare et al, 2023).…”

A Bibliometric and In Silico-Based Analysis of Anti-Lung Cancer Compounds from Sea Cucumber

“…The 18 most investigated apoptotic receptors were obtained from prior studies [ 22 ]. We then utilized AutoDock 4 [ 23 ] to examine the binding affinity of all 22 triterpenes in the extracts of various sea cucumbers that have previously shown anti-lung cancer properties against these apoptotic receptors.…”

Therapeutic, chemo-sensitizing, protective effects of natural products against cancerous cells: Preclinical and clinical studies

“…The cytotoxicity of jellyfish venom, such as that of Pelagia noctiluca against colon cancer cells and Cassiopea andromeda against breast cancer cells, is attributed to the production of reactive oxygen species (ROS), the stimulation of lipid peroxidation, and damage to mitochondria (Ayed et al, 2011). On a variety of cancer cell lines, including those from the brain, colon, lung, and liver, jellyfish venom or isolated peptides have anti-proliferative effects (Zare et al, 2023).…”