Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in Swietenia mahagoni leaf stalks

Fadhillah, Fadhillah; Elfita, Elfita; Muharni, Muharni; Yohandini, Heni; Widjajanti, Hary

doi:10.13057/biodiv/d200929

Cited by 17 publications

(16 citation statements)

References 23 publications

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“…32 The production of the same compound in some endophytic fungi is common. An example is (5-hydroxy-2-oxo-2H pyran-4-yl) methyl acetate produced by the endophytic fungus Cladosporium tenuissimum from S. mahagoni, 20 Trichoderma sp. from Tinospora crispa, 33 and Aspergillus sp from Andrographis paniculata.…”

Section: Resultsmentioning

confidence: 99%

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Antioxidant Compound of Endophytic Fungi Penecillium oxalicum Isolated from Swietenia mahagoni L. Jacq

2021

TJNPR

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Endophytic fungi are microorganisms that are symbionts in plant tissues. Endophytic fungi isolated from medicinal plants such as Swietenia mahagoni may produce a plethora of bioactive compounds. In this study, endophytic fungi was isolated from the leaves of S. mahagoni and cultivated in potato-dextrose broth (PDB) for six weeks at static condition. The antioxidant compound was isolated from the ethyl acetate extract of endophytic fungi by chromatographic method, and the chemical structure was determined by spectroscopic analysis. The antioxidant activity was tested by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and the endophytic fungi was analyzed to identify its species by molecular method. Based on the spectroscopic analysis ( 1 H-NMR, 13 C-NMR, HMQC, and HMBC spectra), the antioxidant compound was identified as mono-o-methylcurvilinic acid with IC50 of 38.01 µg/mL. Phylogenetic analysis showed the endophytic fungi as Penicillium oxalicum.

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Section: Resultsmentioning

confidence: 99%

“…The file was entered into the CONSENSE program. The phylogenetic tree was visualized by the Treeview and Mega4 program 20. Isolation and identification of pure Compound Secondary metabolite of the active extract was separated using column chromatography with silica gel as the stationary phase and a gradient solvent as the mobile phase.…”

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confidence: 99%

Antioxidant Compound of Endophytic Fungi Penecillium oxalicum Isolated from Swietenia mahagoni L. Jacq

2021

TJNPR

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“…The fraction containing potential secondary metabolites was further chromatographed by column and rinsed with a suitable solvent to obtain a pure compound, namely compound 1. The structure of the pure compound (compound 1) was identified by spectroscopic methods, including 1 H-NMR, 13 C-NMR, HMQC, HMBC, and COSY (Fadhillah et al 2019).…”

Section: Isolation and Identification Of Bioactive Compoundmentioning

confidence: 99%

Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos)

et al. 2022

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Abstract. ‘Aini K, Elfita, Widyajanti H, Setiawan A, Kurniawati AR. 2021. Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos). Biodiversitas 23: 521-532. Syzygium jambos (jambu mawar) is widely used in South Sumatra as a traditional medicine to treat various diseases, including pathogenic bacterial infections. Literature studies report that parts of S. jambos have been used worldwide for treating diarrhea accompanied by fever, dysentery, sore throat, diabetes, and other infectious diseases. Endophytic fungi isolated from medicinal plants have high diversity, and the biological activity of their secondary metabolites is associated with their host. Therefore, this study aimed to determine the diversity of endophytic fungi from the stem bark of S. jambos and the antibacterial activity of secondary metabolites of endophytic fungi. Fungal isolates with high antibacterial activity were identified to obtain fungal species, and the structure of their compounds was determined. The disc diffusion method carried the antibacterial activity test against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Salmonella typhi) bacteria. Characteristics of endophytic fungi were identified microscopically and macroscopically. Active endophytic fungi were identified by molecular analysis of the internal transcribed region (ITS) of the ribosomal DNA. Isolation of antibacterial compounds using chromatographic techniques and determination of chemical structures using spectroscopy was performed. Eight endophytic fungi were obtained from the stem bark of S. jambos, namely SJ1–SJ8. The endophytic fungus SJ6 showed the highest activity and base on phylogenetic analysis, was identified as Fusarium verticillioides. Pure compounds isolated as yellowish-white solids showed good antibacterial activity against S. aureus and S. typhi with minimum inhibitory concentration values of 64 g/mL each. Based on 1D and 2D nuclear magnetic resonance spectroscopic analysis, compound 1 was identified as 3-hydroxy-4-(hydroxy(4-hydroxyphenyl)methyl)dihydrofuran-2-on.

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“…The mixture became homogenized and left in a darkish tube for 30 minutes. Absorption was measured the use of a UV-Vis spectrophotometer at 𝜆 max 517 nm (Fadhillah et al, 2019) . In this study, ascorbic acid was used as the standard antioxidant.…”

Section: Antioxidant Activity Analysismentioning

confidence: 99%

Antioxidant and Antibacterial Activities of Endophytic Fungi Extracts of Syzygium zeylanicum

Syarifah

Elfita

Widjajanti

et al. 2022

Sci. Technol. Indones

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Syzygium zeylanicum, one of the other therapeutic plants found in Indonesia, is used to treat various ailments, such as antimicrobial, anti-inflammatory, antioxidant, arthritis, antidiabetic, mosquitocidal, antitumor, and anti-rheumatic agents. The massive use of plant extracts has caused the development of isolation of bioactive compounds thru their endophytic. The present research aimed to obtain endophytic fungal isolates from the stem bark and leaves of Jambu nasi-nasi (S. zeylanicum) and analyze endophytic fungal extracts’ antioxidant and antibacterial activity. Endophyte identification was performed morphologically, and isolates with high biological activity were molecularly characterized. The antibacterial activity was evaluated by the disc diffusion method, and the antioxidant activity was evaluated by the DPPH method. In total, 10 endophytic fungi were isolated and identified as Phialemonium sp. (Code SZT3), Acremonium sp. (Code SZT4), Trichoderma aureoviridae (Code SZT5), Trichoderma koningi (Code SZT7), Phytium torulosum (Code SZL1), Phytium zingiberum (Code SZL2), Septonema sp. (Code SZL3), Lasiodiplodia pseudotheobromae (Code SZL4), Volutella cilliata (SZL5), and Trichophyton mentagrophytes (Code SZL7). Isolate SZL4 gave activity the highest antioxidant (IC50 = 3.30 ????g/mL) and strong antibacterial activity against four pathogens bacterial (S. thypi, B. subtilis, S. aureus, and E. coli). The potential endophytic fungi based on molecular analysis was Lasiodiplodia pseudotheobromae with accession number OK668257. These endophytic fungi can be developed into new sources of antibacterial and antioxidant bioactive compounds through further studies.

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Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in Swietenia mahagoni leaf stalks

Cited by 17 publications

References 23 publications

Antioxidant Compound of Endophytic Fungi Penecillium oxalicum Isolated from Swietenia mahagoni L. Jacq

Antioxidant Compound of Endophytic Fungi Penecillium oxalicum Isolated from Swietenia mahagoni L. Jacq

Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos)

Antioxidant and Antibacterial Activities of Endophytic Fungi Extracts of Syzygium zeylanicum

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