Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions

Abstract

The effect of carbon and nitrogen sources and initial pH and temperature of the medium on the mycelial growth of Sarcodon imbricatus (L.) P. Karst. in axenic liquid culture was investigated. The optimal composition of the medium was found to be: 5% fructose, 1% hydrolysate of casein, 1% yeast extract, and 0.3% KH 2 PO 4 at pH = 6 and incubation temperature of 20°C. In this condition the maximum biomass growth was observed, yielding 10.2 g L −1 of dry weight after 3-week of growth. The medium regarded as optimal for growth of S. imbricatus mycelium was used for the production of the biomass and further chemical analysis. The quantitative and qualitative composition of phenolic acids, fatty acids, and sterols were determined using chromatographic methods. The total content of phenolic acids was 1.86 mg × 100 g −1 DW, with the largest amount of protocatechuic acid (1.27 mg × 100 g −1 DW). Nineteen fatty acids were estimated, including five unsaturated fatty acids, e.g., oleic and α-linolenic acid. The analysis of sterols composition revealed the presence of ergosterol and ergosterol peroxide (197.7 and 200.47 mg × 100 g −1 DW, respectively). These compounds were isolated and confirmed by 1 H-NMR. Presented study constitutes the first report on the accumulation of substances (phenolic acids, fatty acids, and sterols) with multidirectional biological activity in the mycelial axenic culture of Sarcodon imbricatus.

Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise‐Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2‐Mediated Oxidative Stress

“…The advantages of submerged fermentation have been reported widely such as the shorter growth cycle, stability chemical composition, and controllable biosynthesis processes. Encouragingly, the optimum submerged fermentation conditions for SI mycelium culture have been obtained by the previous study [ 21 ]. In our subsequent experiments, the differences in antifatigue activities between the SI fruiting body and SI mycelia will be investigated.…”

“…Sarcodon imbricatus (SI), belonging to the family of Basidiomycotina and Aphyllophorales , is an edible and medicinal fungus, widely distributed in Central Europe and in North America [ 18 ] and also produced in Tibet, Gansu, Anhui, northwestern Yunnan, and western Sichuan of China [ 19 ]. Although SI is described to show various pharmacological activities including anti-inflammation and anticancer in the folk, previous studies mainly focused on its chemical component analysis and polysaccharide isolation [ 20 , 21 ]. Our group has already confirmed the immunomodulatory property of S. imbricatus water extracts in the cyclophosphamide- (CTX-) induced immunosupressive mouse model, which is related to its modulation on oxidative stress [ 22 ].…”

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

“…An alternative method to obtain secondary metabolites with therapeutic properties from fruiting bodies is to use the biosynthetic capability of mycelial cultures. The main advantage of mycelial cultures is their independence from environmental conditions and their ability to continuously produce high-quality material; however, there may be differences in the chemical composition of mycelium and fruiting bodies [ 7 ].…”

Analysis of the biodegradation of synthetic testosterone and 17α-ethynylestradiol using the edible mushroom Lentinula edodes

“…The mean ergosterol content in the fruiting bodies of genus Lactarius is 2.69–3.00 mg/g d.w. and in Cantharellus 3.04–3.77 mg/g d.w. In turn in mycelial cultures of Sarcodon imbricatus amounts of ergosterol and ergosterol peroxide were 1.97 mg/g d.w. and 2.00 mg/g d.w., respectively (Sułkowska-Ziaja et al 2016 ). It was proven that these compounds are essential for the normal development of hyphae of higher fungi while ergosterol is the main part of fungal cell membranes.…”