CHEMICAL CONSTITUENTS AND ACETYLCHOLINESTERASE ACTIVITY FROM Polyalthia sumatrana (Miq.) Kurz

Abstract

This study was carried out to investigate the phytochemicals from Polyalthia sumatrana and their acetylcholinesterase inhibitory activity. Fractionation and purification of the leaves of P. sumatrana led to the isolation and identification of five alkaloids; boldine (1), norboldine (2), liriodenine (3), predicentrine (4), laurotetanine (5) together with β-sitosterol (6), β-sitostenone (7), vanillin (8), vanillic acid (9) and cinnamic acid (10). The structures of these compounds were obtained by analysis of their spectroscopic data, as well as the comparison with that of reported data. Acetylcholinesterase inhibitory activity revealed that all isolated alkaloids were found to inhibit AChE with percentage inhibition values ranged from 45.0 to 80.6%.

SECONDARY METABOLITES FROM THE STEM BARK OF POLYALTHIA STENOPETALA (HOOK. F. & THOMSON) RIDL. AND THEIR ACETYLCHOLINESTERASE ACTIVITY

“…Studies on the stem bark of P. stenopetala species were conducted in light of the reported medicinal benefits leading to isolation and structure elucidation of ten metabolites including alkaloids, lignans, and phenolics. Isolated compounds were identified as laurotetanine (1), N-methyllaurotetanine (2), reticuline (3), liriodenine (4), yangambin (5), syringaresinol (6), vanillic acid (7), 4hydroxybenzoic acid (8), cinnamic acid (9), and syringic acid (10), as shown in Figure 1.…”

“…AChE inhibitory activities were measured using a spectrophotometric method [9] with electric eel AChE, acetylthiocholine iodides and 5,5'-Dithio-bis(2-nitrobenzoic) acid (DTNB) were employed. Briefly, sodium phosphate buffer (140 μL, pH 8.0), DTNB (20 μL), tested compound (20 μL, 1 mg/mL) and of AChE solution (20 μL) were incubated in a 96well microplate (15 min, 25°C).…”

“…Polyalthia species such as P. sumatrana 9 , P. cauliflora 10 , P. laterifolia 11 , and P. insignis 12 . Meanwhile, lignans were also reported from P. rumphii 13 , and compound (5) was firstly reported from the genus Polyalthia.…”

The impact of seasonal variation on the composition of the volatile oil of Polyalthia suberosa (Roxb.) Thwaites leaves and evaluation of its acetylcholinesterase inhibitory activity

“…To the best of our knowledge, the AChE inhibitory activity of PSLO has never been reported before. However, some alkaloids isolated from P. stenopetala and P. sumatrana inhibited AChE with percentage inhibition values ranging from 40.2 to 80.6% [51,52].…”

Colorimetric sensing of calcium carbide over banana peels using 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) as a rapid chemoreceptor: a point of care tool for food fraud analysis

“…The DTNB assay is used to assess enzyme activity; it detects sulfide bonds and targets them for cleavage, resulting in ions that are responsible for colorimetric signals. 49,50 To monitor the presence of CaC 2 in banana peels, we focused on the high sulfide impurity ''CaS'' present in commercial CaC 2 , [4][5][6] and a new method was validated, and a solution-based easy colorimetric CaC 2 sensor was developed using the DTNB-based method as 1st screening for artificially ripened bananas. As a proof of concept for real-time detection, bananas were used as the model sample in this study.…”