Chemical Constituents and Antiulcer Activity of n-Hexane Extract of Sanchezia nobilis Hook F. Leaves from Vietnam

Abstract

Five compounds (1-5) were isolated from the leaves of Sanchezia nobilis collected in Nam Dinh province by chromatographic methods. These compounds were identified as: mangiferin (1), β-sitosterol (2), margaric acid (3), ursolic acid (4), oleanolic acid (5). Their structures were elucidated by spectroscopic methods, including mass spectrometry and nuclear magnetic resonance. These compounds were isolated from the leaves of Sanchezia nobilis for the first time. Screening of gastric and duodenal antiulcer effects on indomethacin induced gastric ulcer models showed that n-hexane fraction produced the highest antiulcer activity. Percentage inhibition of gastric ulceration of misoprostol was 22.86 %, while that of n-hexane fraction was 28.57 % (p < 0.05). Evaluation of gastric and duodenal antiulcer effects on acysteamine induced gastric ulcer models showed that this fraction was effective against gastric and duodenal ulcer (83.3 %), improved ulcer damage (54.17 %), significantly reduced the number of mean ulcer and ulcer index (2.00 ± 1.28) but it did not change the area of the ulcer.

GC-MS and HPLC-ESI-MS-MS Characterization of Sanchezia oblonga (Acanthaceae) Extracts

“…In this work, phytosterols and kaempferol glycosides were detected only in stems. However, a recent work reported that phytosterols were isolated from n-hexane and ethylacetate extract of the leaves (Xuan et al, 2019a, while kaempferol glycosides were isolated from ethylacetate and aqueous extracts of leaves of S. nobilis (syn. S. oblonga) (Xuan et al, 2019b).…”

“…An earlier study reported that S. nobilis extracts contain syringin, flavone glycosides, cinnamyl and benzyl alcohol glycosides, neolignan glucoside, besides matsutake alcohol glycosides (Ellah et al 2013(Ellah et al , 2014. The presence of the phytosterols daucosterol and stigmasterol, flavonol glycosides, scopoletin and 3'-O-methyl-3,4methylenedioxy ellagic acid in ethylacetate extract of the leaves of S. nobilis was reported recently (Xuan et al, 2019a. On the other hand, mangiferin, β-sitosterol, in addition to margaric, ursolic and oleanolic acids were isolated from n-hexane extracts (Xuan et al, 2019b), while 4',5,7-trihydroxy-3',5'-dimethoxyflavone and kaempferol glycosides were isolated from aqueous extracts of leaves (Loi et al, 2019b).…”