Chemical Constituents, and their Cytotoxicity, of the Rare Wood Decaying Fungus <i>Xylaria humosa</i>

Sodngam, Sirirath; Sawadsitang, Sasiphimol; Suwannasai, Nuttika; Mongkolthanaruk, Wiyada

doi:10.1177/1934578x1400900205

Cited by 9 publications

(8 citation statements)

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“…[ 13 22 ] Compounds 1 to 5 were tested for the in vitro growth inhibition against MCF7, NCI-H460, and A375-C5 cancer cell lines, with compounds 2, 3, 4b demonstrating effect in the three cell lines[ 13 ] and compound 5 in MCF7 cells. [ 23 ] Interestingly, aszonapyrone A which is a constituent of both E2 and E3, was considered to be the most potent compound with GI 50 10 μM for all the cell lines tested. Consequently, these compounds and, possibly others, may be responsible for the anti-proliferative effect of E2 and E3.…”

Section: Resultsmentioning

confidence: 99%

Crude extracts of marine-derived and soil fungi of the genus Neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines

et al. 2016

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Background:The crude ethyl acetate extracts of marine-derived fungi Neosartorya tsunodae KUFC 9213 (E1) and N. laciniosa KUFC 7896 (E2), and soil fungus N. fischeri KUFC 6344 (E3) were evaluated for their in vitro anticancer activities on a panel of seven human cancer cell lines.Materials and Methods:The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed, after 48 h treatments with different concentrations of extracts, to determine their concentration of the extract or Dox that inhibits cell viability by 50% for each cell line. The effects of the crude extracts on DNA damage, clonogenic potential and their ability to induce cell death were also assessed.Results:E1 was found to the void of anti-proliferative effects. E2 was shown to decrease the clonogenic potential in human colorectal carcinoma cell line (HCT116), human malignant melanoma cell line (A375), human breast adenocarcinoma cell line (MCF7), and human caucasian colon adenocarcinoma Grade II cell line (HT29) cells, whereas E3 showed such effect only in HCT116 and MCF7 cells. Both extracts were found to increase DNA damage in some cell lines. E2 was found to induce cell death in HT29, HCT116, MCF7, and A375 cells while extract E3 increased cell death in MCF7 and HCT116 cell lines.Conclusion:The results reveal that E2 and E3 possess anticancer activities in human colon carcinoma, breast adenocarcinoma, and melanoma cells, validating the interest for an identification of molecular targets involved in the anticancer activity.SUMMARY The crude ethyl acetate extract of N. tsunodae (E1) did not decrease cell viability in any of the tested cell linesThe crude ethyl acetate extracts of N. laciniosa (E2) and N. fischeri (E3) decreased cell proliferation in some human cancer cell lines tested at both short- and long-termN. laciniosa (E2) induced a significant increase in the number of cell death, in part, due to the induction of DNA damageN. fischeri (E3) induce cell death but in some cell lines without induction of DNA damage detected by comet assayCrude ethyl extracts of N. laciniosa (E2) and N. fischeri (E3) exert an anticancer activity in human colon carcinoma, breast adenocarcinoma, and malignant melanoma cells. Abbreviations Used: A375: Human malignant melanoma cell line; A549: Human non-small lung cancer cell line; DAPI: 4,6-diamidino-2-phenylindole; DMEM: Dulbecco's Modified Eagle Medium; DMSO: Dimethylsulfoxide; Dox: Doxorubicin; E1: Neosartorya tsunodae KUFC 9213; E2: Neosartorya laciniosa KUFC 7896; E3: Neosartorya fischeri KUFC 6344; FBS: Fetal bovine serum; HCT116: Human colorectal carcinoma cell line; HEPES: (N-[2-hydroxyethyl] piperazine-N’-[2-ethane-sulfonic acid]); HepG2: Human hepatocellular carcinoma cell line; HT29: Human caucasian colon adenocarcinoma Grade II cell line; IC50: Concentration of the extract or Dox that inhibits cell viability by 50%; MCF7: Human breast adenocarcinoma cell line; MEM: Minimum Essential Medium Eagle; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NCI-H460: Human non-...

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Section: Resultsmentioning

confidence: 99%

Crude extracts of marine-derived and soil fungi of the genus Neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines

et al. 2016

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“…Most of the compounds (except 8 , 9 , 12 , and 19 ) could have potential to be used in effective concentrations without toxicity. Previous studies have shown that 7 [ 47 ], 13 , and 14 [ 48 ] have also displayed cytotoxicity in other cell lines.…”

Section: Resultsmentioning

confidence: 99%

Metabolites from Marine-Derived Fungi as Potential Antimicrobial Adjuvants

et al. 2021

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Marine-derived fungi constitute an interesting source of bioactive compounds, several of which exhibit antibacterial activity. These acquire special importance, considering that antimicrobial resistance is becoming more widespread. The overexpression of efflux pumps, capable of expelling antimicrobials out of bacterial cells, is one of the most worrisome mechanisms. There has been an ongoing effort to find not only new antimicrobials, but also compounds that can block resistance mechanisms which can be used in combination with approved antimicrobial drugs. In this work, a library of nineteen marine natural products, isolated from marine-derived fungi of the genera Neosartorya and Aspergillus, was evaluated for their potential as bacterial efflux pump inhibitors as well as the antimicrobial-related mechanisms, such as inhibition of biofilm formation and quorum-sensing. Docking studies were performed to predict their efflux pump action. These compounds were also tested for their cytotoxicity in mouse fibroblast cell line NIH/3T3. The results obtained suggest that the marine-derived fungal metabolites are a promising source of compounds with potential to revert antimicrobial resistance and serve as an inspiration for the synthesis of new antimicrobial drugs.

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“…Sodngam et al () have also described variable cytotoxic activity of chevalone C ( 2 ), fiscalin A ( 8 ), epi ‐fiscalin C ( 13 ) and epi ‐fiscalin A ( 9 ), isolated from wood‐decomposing fungus Xylaria humosa in NCI‐H187 (small cell lung cancer), on MCF‐7 (breast adenocarcinoma) and KB (mouth epidermal carcinoma) cancer cell lines. Compound 2 was active in KB and NCI‐H187, with an IC 50 of 49.4 and 17.7 µg/mL, respectively.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of Secondary Metabolites from Marine‐derived Fungus Neosartorya siamensis in Human Cancer Cells

Prata‐Sena

Ramos

Buttachon

et al. 2016

Phytotherapy Research

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Compounds isolated from the marine sea fan-derived fungus Neosartorya siamensis (KUFA 0017), namely, 2,4-dihydroxy-3-methylacetophenon (1), chevalone C (2), nortryptoquivaline (4), tryptoquivaline H (6), tryptoquivaline F (7), fiscalin A (8), epi-fiscalin A (9), epi-neofiscalin A (11) and epi-fiscalin C (13) were tested for anti-proliferative activity by MTT assay, DNA damage induction by comet assay, and induction of cell death by nuclear condensation assay on colon HCT116, liver HepG2 and melanoma A375 cancer cell lines. Compounds 2, 4, 8, 9, 11 and 13 presented IC values ranging from 24 to 153 μM in the selected cell lines. Cell death was induced in HCT116 by compounds 2, 4 and 8. In HepG2, compounds 4, 8, 9 and 11 were able to induce significant cell death. This induction of cell death is possibly not related to genotoxicity because none of the compounds induced significant DNA damage. These results suggest that selected compounds present an interesting anti-proliferative activity and cell death induction, consequently showing potential (specifically epi-fiscalin C) as future leads for chemotherapeutic agents. Further studies on mechanisms of action should ensue. Copyright © 2016 John Wiley & Sons, Ltd.

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Chemical Constituents, and their Cytotoxicity, of the Rare Wood Decaying Fungus Xylaria humosa

Cited by 9 publications

References 10 publications

Crude extracts of marine-derived and soil fungi of the genus Neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines

Crude extracts of marine-derived and soil fungi of the genus Neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines

Metabolites from Marine-Derived Fungi as Potential Antimicrobial Adjuvants

Cytotoxic activity of Secondary Metabolites from Marine‐derived Fungus Neosartorya siamensis in Human Cancer Cells

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