Crude extracts of marine-derived and soil fungi of the genus Neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines

Abstract

Background:The crude ethyl acetate extracts of marine-derived fungi Neosartorya tsunodae KUFC 9213 (E1) and N. laciniosa KUFC 7896 (E2), and soil fungus N. fischeri KUFC 6344 (E3) were evaluated for their in vitro anticancer activities on a panel of seven human cancer cell lines.Materials and Methods:The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed, after 48 h treatments with different concentrations of extracts, to determine their concentration of the extract or Dox that inhibits cell viability by 50% for each cell line. The effects of the crude extracts on DNA damage, clonogenic potential and their ability to induce cell death were also assessed.Results:E1 was found to the void of anti-proliferative effects. E2 was shown to decrease the clonogenic potential in human colorectal carcinoma cell line (HCT116), human malignant melanoma cell line (A375), human breast adenocarcinoma cell line (MCF7), and human caucasian colon adenocarcinoma Grade II cell line (HT29) cells, whereas E3 showed such effect only in HCT116 and MCF7 cells. Both extracts were found to increase DNA damage in some cell lines. E2 was found to induce cell death in HT29, HCT116, MCF7, and A375 cells while extract E3 increased cell death in MCF7 and HCT116 cell lines.Conclusion:The results reveal that E2 and E3 possess anticancer activities in human colon carcinoma, breast adenocarcinoma, and melanoma cells, validating the interest for an identification of molecular targets involved in the anticancer activity.SUMMARY The crude ethyl acetate extract of N. tsunodae (E1) did not decrease cell viability in any of the tested cell linesThe crude ethyl acetate extracts of N. laciniosa (E2) and N. fischeri (E3) decreased cell proliferation in some human cancer cell lines tested at both short- and long-termN. laciniosa (E2) induced a significant increase in the number of cell death, in part, due to the induction of DNA damageN. fischeri (E3) induce cell death but in some cell lines without induction of DNA damage detected by comet assayCrude ethyl extracts of N. laciniosa (E2) and N. fischeri (E3) exert an anticancer activity in human colon carcinoma, breast adenocarcinoma, and malignant melanoma cells. Abbreviations Used: A375: Human malignant melanoma cell line; A549: Human non-small lung cancer cell line; DAPI: 4,6-diamidino-2-phenylindole; DMEM: Dulbecco's Modified Eagle Medium; DMSO: Dimethylsulfoxide; Dox: Doxorubicin; E1: Neosartorya tsunodae KUFC 9213; E2: Neosartorya laciniosa KUFC 7896; E3: Neosartorya fischeri KUFC 6344; FBS: Fetal bovine serum; HCT116: Human colorectal carcinoma cell line; HEPES: (N-[2-hydroxyethyl] piperazine-N’-[2-ethane-sulfonic acid]); HepG2: Human hepatocellular carcinoma cell line; HT29: Human caucasian colon adenocarcinoma Grade II cell line; IC50: Concentration of the extract or Dox that inhibits cell viability by 50%; MCF7: Human breast adenocarcinoma cell line; MEM: Minimum Essential Medium Eagle; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NCI-H460: Human non-...

Wild Medicinal Fungi: Phallus Impudicus-induced Telomere Damage Triggers p21/p53 and p16-dependent Cell Cycle Arrest and Drives to Male Fertility Reduction in Vitro

“…The obtained result may be associated with various types of cells tested and their other physiological functions. This is consistent with other reports, which demonstrate that different fungal extracts inhibit cell proliferation in a cell-speci c dependent manner at both short-and long-term (Ramos et al 2016;Wu et al 2014). Furthermore, fungi extracts have an antiproliferation effect on tumors through various intracellular signaling pathways.…”

Marine-derived fungi extracts enhance the cytotoxic activity of doxorubicin in nonsmall cell lung cancer cells A459

“…[ 31 32 ] We recently demonstrated that Dox can decrease cell viability in several cell models, and in some of them, induction of DNA damage and cell death occur. [ 16 ] However, one of the most resistant cell lines with higher IC 50 was A549 cell. [ 17 ] In this study, the results show that Dox at 0.54 μM decreased cell viability of A549 cells with induction of cell death being accomplished with nuclear condensation but without induction of strand breaks detected by comet assay.…”

“…Neosartorya tsunodae KUFC 9213 (E1) was isolated from a marine sponge, Aka coralliphaga , Neosartorya laciniosa KUFC 7896 (E2) was isolated from a diseased coral (ulcerative white spot in Porites lutea ), and Neosartorya fischeri KUFC 6344 (E3) was isolated from coastal forest soil, as described by Ramos et al . [ 16 ] Aspergillus similanensis KUFA 0013 (E4) was isolated from a marine sponge, Rhabdermia sp., Neosartorya paulistensis KUFC 7894 (E5) was isolated from a marine sponge, Chondrilla australiensis , and Talaromyces trachyspermum KUFC 0021 (E6) was isolated from a marine sponge, Clathria reianwardii , as reported by Ramos et al . [ 17 ]…”

“…N. tsunodae , N. laciniosa , N. fischeri , A. similanensis , N. paulistensis , and T. trachyspermus were cultured, and the crude ethyl extracts were prepared according to our previous report. [ 16 17 ] Briefly, the fungi were cultured for 1 or 2 weeks in Petri dishes with potato dextrose agar (strain 6344) or malt extract agar (strains 9213, 7896, 0013, 7894, and 0021). Autoclaved Erlenmeyer flasks, containing water and rice, were inoculated with mycelial plugs of the fungi and incubated for 30 days at 28°C.…”

Effect of Marine-Derived Saccharides on Human Skin Fibroblasts and Dermal Papilla Cells

“…Marine organisms are a source of many ingredients which have various useful roles in maintaining health. They can be applied in the fight against reactive oxygen species (ROS) [ 13 , 14 , 15 , 16 , 17 ] and in the treatment or prevention of infectious diseases [ 18 , 19 , 20 , 21 ], cancer diseases [ 22 , 23 , 24 , 25 , 26 ], diabetes [ 27 , 28 ], and obesity [ 29 , 30 , 31 ], as well as cardiovascular illnesses caused by hypertension [ 32 , 33 ]. Marine-derived compounds are used to produce drugs, pharmaceuticals, and supplements, as well as cosmetics.…”