Chemical Constituents from Endophytic Fungus Annulohypoxylon cf. stygium in Leaves of Anoectochilus roxburghii

Abstract

The chemical investigation on endophytic fungus Annulohypoxylon cf. stygium in leaves of Anoectochilus roxburghii (Wall.) Lindl. has been performed. Sixteen compounds were isolated and their structures were identified as (−)‐notoamide A, (−)‐notoamide B, (+)‐versicolamide B, notoamide C, notoamide D, stephacidin A, sterigmatocystin, dihydrosterigmatocystin, secosterigmatocystin, versiconol, averufanin, kipukasin D, kipukasin E, diorcinal, palmarumycin CP2 and (−)‐(3R)‐mellein methyl ether, respectively, by spectroscopic analysis and comparison with literature data. All the compounds were isolated from Annulohypoxylon genus for the first time. Sterigmatocystin and palmarumycin CP2 showed selective cytotoxic activities against HepG2, HeLa, MCF‐7 and HT‐29.

The Biosynthesis, Structure Diversity and Bioactivity of Sterigmatocystins and Aflatoxins: A Review

“…In addition, compound 31 exhibited significant cytotoxicity against human solid tumour cell lines (A549, SK-OV-3, SK-MEL-2, XF-498 and HCT-15) with IC 50 values ranging from 0.41–4.61 µg/mL [ 47 ]. Meanwhile, compound 31 showed strong cytotoxicity against L5178Y, BT-549, HepG2, HGC-27, UMUC-3, MCF-7, Hela, HT-29, HCT-116 and other cell lines, with IC 50 values ranging from 0.3–58 µM [ 45 , 48 , 49 , 50 , 51 ]. There are many studies on the antitumour activity of compound 31 , and the mechanism of action of compound 31 mainly focuses on the induction of the G2 phase block in GES-1 cells.…”

Deep-Sea Natural Products from Extreme Environments: Cold Seeps and Hydrothermal Vents

“…Sterigmatocystin (47) displayed promising antibacterial activity, especially on Pseudomonas aeruginosa, with a MIC of 125 µg/mL [41]. Sterigmatocystin (47) also showed cytotoxic activities against HepG2, Hela, MCF-7, and HT-29, with IC 50 values of 12.50 ± 0.89 µM, 11.50 ± 0.99 µM, 6.76 ± 0.31 µM, and 8.16 ± 0.39 µM, respectively [42]. Curvularin (48) was active against fungi and numerous cancer cell lines [43], and the total synthesis of curvularin (48) was achieved through a ring-closing-metathesisbased construction of the macrocyclic framework [44].…”