Chemical Constituents from the Roots of Ranunculus ternatus and their Inhibitory Effects on Mycobacterium tuberculosis

Ke-zhong, Deng; Xiong, Ying; Zhou, Bin; Guan, Yongmei; Luo, Yongming

doi:10.3390/molecules181011859

Cited by 14 publications

(12 citation statements)

References 12 publications

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“…Currently, it is mainly used in the treatment of tuberculosis and lymph node tuberculosis, as well as adjuvant therapy for cancer (Deng et al. 2013 ; Sun et al. 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Ranunculus ternatus Thunb extract attenuates renal fibrosis of diabetic nephropathy via inhibiting SMYD2

Peng

Chen

et al. 2022

Pharmaceutical Biology

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Context Ranunculus ternatus Thunb (Ranunculaceae), (RTT) is used clinically for the treatment of tuberculosis or as tumour adjuvant therapy, but its potential effect on diabetic nephropathy (DN) has not been studied. Objective To investigate the effect of RTT extract in renal fibrosis of DN. Materials and methods C57BL/6 mice were randomly divided into four groups ( n = 12). Diabetes mellitus (DM) mice were induced by streptozotocin (STZ, 55 mg/kg/day) for five consecutive days and treated by RTT extract (2 g/kg). Afterward, blood glucose, HE and Masson staining were assayed. The expression levels of Vimentin, ɑ-SMA, TNF-ɑ, NF-κB p-p65, NF-κB p65, SMYD2, H3K36me3, H3K4me3 were determined by western blots. Firbronectin was respectively assayed by western blot and immunofluorescent staining. Results RTT extract significantly ameliorated renal injury and renal fibrosis in the renal tissue of STZ-induced diabetic mice as demonstrated by the decreased expression level of Fibronectin (65%), Vimentin and α-SMA (75% & 53%). In addition, the levels of TNF-α (57%), NF-κB p-p65 and NF-κB p65 (35% & 25%) were elevated in the DN mice. Importantly, these were alleviated after RTT extract treatment. Moreover, we observed that the protein levels of SMYD2 (30%), H3K36me3 and H3K4me3 (53% & 75%) were reduced in DN mice after treatment with RTT extract. Discussion and conclusions RTT extract mediates antifibrotic effects and anti-inflammatory responses in STZ-induced DN mainly through suppressing SMYD2 activation and H3K36me3 and H3K4me3 protein expression. RTT extract might have therapeutic potential against high glucose-induced nephropathy.

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“…Currently, it is mainly used in the treatment of tuberculosis and lymph node tuberculosis, as well as adjuvant therapy for cancer (Deng et al. 2013 ; Sun et al. 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Ranunculus ternatus Thunb extract attenuates renal fibrosis of diabetic nephropathy via inhibiting SMYD2

Peng

Chen

et al. 2022

Pharmaceutical Biology

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“…Radix Ranunculus ternati , a traditional Chinese herbal medicine, has been used to treat numerous types of disease and as an adjuvant therapy for cancer many years ( 19 ). The pharmacology of Radix R. ternate depend on saponins and polysaccharides ( 20 , 21 ). However, further evidence is needed to prove their anti-tumor effect.…”

Section: Discussionmentioning

confidence: 99%

“…In traditional Chinese medicine, radix Ranunculus ternati is applied in the treatment of numerous types of disease, including scrofula, tuberculosis and pharyngitis ( 19 ), and comprises saponins, polysaccharides and certain fatty substances ( 20 , 21 ). Radix ranunculus temate saponins (RRTS) has shown satisfactory in vitro ability to suppress gastric cancer cells growth and proliferation ( 22 , 23 ).…”

Section: Introductionmentioning

confidence: 99%

Radix ranunculus temate saponins sensitizes ovarian cancer to Taxol via upregulation of miR‑let‑7b

et al. 2022

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A common cause of treatment failure in ovarian cancer is acquired drug resistance. Therefore, effective novel drugs against chemoresistance need to be developed. MicroRNAs (miRNAs or miRs) serve key regulatory roles in tumorigenesis and chemoresistance. The objective of the present study was to explore the role of miR-let-7b in ovarian cancer chemoresistance, and to develop novel strategy for the treatment of drug-resistant ovarian cancer. For this purpose, reverse transcription-quantitative PCR was performed to evaluate the expression level of miR-let-7b in fresh ovarian cancer tissues and cell lines. miR-let-7b mimic was transfected into ovarian cancer cell lines. Functional experiments, cell apoptosis and cell viability assays were carried out to identify the tumor-suppressor function of miR-let-7b. The treatment effect of Radix ranunculus temate saponins (RRTS), one of the primary constituents extracted from the traditional Chinese medicine radix Ranunculi ternati, was identified in vitro and in vivo. The results revealed that miR-let-7b was downregulated significantly in chemoresistant ovarian cancer patients. miR-let-7b overexpression suppressed cell growth and invasion and enhanced sensitivity to Taxol of ovarian cancer cells. Furthermore, miR-let-7b levels in ovarian cancer tissue were inversely associated with collagen type III α1 chain (COL3A1) levels. COL3A1, a non-fibrillar collagen associated with chemoresistance, was targeted by miR-let-7b. RRTS showed cytotoxic effects on ovarian cancer cells through inducing miR-let-7b expression and decreasing COL3A1 expression.In addition, RRTS sensitized ovarian cancer to Taxol both in vitro and in vivo. In conclusion, the present results revealed synergistic cytotoxicity of RRTS and Taxol on against ovarian cancer cells via upregulating expression of miR-let-7b. Combination of Taxol and RRTS may be a novel treatment strategy for patients with TR ovarian cancer.

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“…is one kind of traditional Chinese medicines with a long history and has been reported to have pharmacological activities, such as anti-inflammatory, analgesic, and other effects. [20][21][22] Our screening from a TCMs pool for anti-HBV activities showed the ethanol extract of R. japonicus Thunb. could inhibit the production of HBeAg and showed anti-HBV potency.…”

Section: R Japonicusmentioning

confidence: 99%

Inhibitory activities of Ranunculus japonicus Thunb. ethanol extract against hepatitis B virus

Luo

Chen

Liu

et al. 2022

Journal of Medical Virology

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The chronic hepatitis B virus (HBV) infection is a worldwide public health problem, which cannot be cured by current therapeutics due to the persistence of viral CCC DNA in the infected hepatocytes. Screening from medicinal herbs for anti-HBV activities showed that the ethanol extract from Ranunculus japonicus Thunb. could decrease the production of HBV e antigen (HBeAg). Further study showed that the extract had no effect on core protein expression but significantly reduced the efficiency of viral capsid assembly. The levels of viral pgRNA and total core DNA were not affected significantly. However, the ratio of RC DNA/SS DNA decreased, indicating that the conversion of RC DNA from SS DNA was delayed by the extract.More interestingly, though similar levels of RC DNA were accumulated, the CCC DNA level and its formation efficiency were reduced significantly, which was also consistent with the decreased level of HBeAg, indicating that R. japonicus Thunb. extract could inhibit the CCC DNA formation. Together, this study found that R. japonicus Thunb. extract could inhibit HBV replication at multiple steps, especially showed significant inhibitory effects on capsid assembly and CCC DNA formation.

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Chemical Constituents from the Roots of Ranunculus ternatus and their Inhibitory Effects on Mycobacterium tuberculosis

Cited by 14 publications

References 12 publications

Ranunculus ternatus Thunb extract attenuates renal fibrosis of diabetic nephropathy via inhibiting SMYD2

Ranunculus ternatus Thunb extract attenuates renal fibrosis of diabetic nephropathy via inhibiting SMYD2

Radix ranunculus temate saponins sensitizes ovarian cancer to Taxol via upregulation of miR‑let‑7b

Inhibitory activities of Ranunculus japonicus Thunb. ethanol extract against hepatitis B virus

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