Chemical constituents isolated from <i>Polygala japonica</i> leaves and their inhibitory effect on nitric oxide production <i>in vitro</i>

Kim, Seung Hyun; Jang, Sin Duk; Lee, Ki Yong; Sung, Sang Hyun; Kim, Young Choong

doi:10.1080/14756360802051362

Cited by 15 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Though the antioxidant activity of kaempferol derivatives on DPPH radical scavenging assay was moderate, they were reported to have inhibitory activities on lipopolysaccharide-induced nitric oxide production in BV2 microglia (Kim, Jang, Lee, Sung, & Kim, 2009), CB 1-6 V and HSV-1 virus infection (Yao & Wang, 1999), and protein kinase CK 2 in vitro and in HL-60 cells (Lin, Liu, Chen, & Liang, 2006). Glycosylated kaempferol derivatives were also reported from some vegetables and fruits, e.g.…”

Section: Dpph Radical Scavenging Capacitymentioning

confidence: 97%

Kaempferol acetylated glycosides from the seed cake of Camellia oleifera

Gao

Zhao

et al. 2011

Food Chemistry

View full text Add to dashboard Cite

Section: Dpph Radical Scavenging Capacitymentioning

confidence: 97%

Kaempferol acetylated glycosides from the seed cake of Camellia oleifera

Gao

Zhao

et al. 2011

Food Chemistry

View full text Add to dashboard Cite

“…(Du et al, 2013;Kim et al, 2009;Wang et al, 2007;Xiang et al, 2005). In the present study, it was found that compounds C1 and C2 could significantly reverse decreased expression of a7 and a3 nAChRs and C2 also could significantly increase expression of SPY in Ab 1-42 -induced SH-SY5Y cells, which indicating that compounds C1 and C2 effectively have protective roles against Ab-induced neurotoxicity.…”

Section: Identification Of Permeable Componentsmentioning

confidence: 50%

Membrane permeability-guided identification of neuroprotective components fromPolygonum cuspidatun

Xiao¹,

Liang³

et al. 2013

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Polygonum cuspidatum Sieb et Zucc. (Polygonaceae) possesses various pharmacological activities and has been widely using as one of the most popular and valuable Chinese herbal medicines in clinics. Its usage has increasingly attracted much of our attention and urges investigation on its bioactive components. Objective: To establish a rapid and valid approach for screening potential neuroprotective components from P. cuspidatum. Materials and methods: Potential neuroprotective components from P. cuspidatum were screened utilizing liposome equilibrium dialysis followed by high-performance liquid chromatography (HPLC) analysis. Their neuroprotective effects on modulation of protein expression of a7 nAChR, a3 nAChR and synaptophysin (SPY) on SH-SY5Y human neuroblastoma cell line (SH-SY5Y) were evaluated by means of Western blotting. Results: Two potential compounds, polydatin (C1) and emodin-8-O-b-D-glucoside (C2), were detected and identified in our study. The biological tests showed that both compounds C1 and C2, respectively, at concentrations of 0.1 and 0.25 mg/mL significantly increased protein expression of a7 and a3 nicotinic acetylcholine receptors (nAChRs) in SH-SY5Y cells. Moreover, C1 and C2 at 0.1 mg/mL significantly reversed the Ab 1-42 -induced decrease of a7 and a3 nAChRs protein expression in SH-SY5Y cells. In addition, C2 at 0.1 mg/mL significantly increased protein expression of SPY in SH-SY5Y cells and Ab 1-42 -induced SH-SY5Y cells whereas C1 did not provide any positive effects. Discussion and conclusion: In conclusion, our approach utilizing liposome equilibrium dialysis combined with HPLC analysis and cell-based assays is a prompt and useful method for screening neuroprotective agents.

show abstract

“…Triterpenoid saponins from the roots of P. japonica Houtt. have anti-inflammatory activity through inhibition of nitrite and PGE 2 production, iNOS and, COX-2 protein expression and TNF-α, IL-1β, IL-6, and IL-12 mRNA expression in LPS-stimulated BV2 cells (Kim et al 2009;Ahn et al 2005). Previous research has reported that PSF inhibit the secretion of inflammatory cytokines induced by LPS and its mechanism is associated with the NF-κB signaling pathway (Wei et al 2014;Yan et al 2015), but the effect and mechanism vary in different conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Polygala japonica Houtt (Gua-zi-jin in Chinese) is used in traditional medicine for the treatment of various inflammatory disorders such as acute tonsillitis, pharyngitis and pulmonitis (Wang et al, 2008). Previous studies have reported triterpenoid saponins from P. japonica exhibited the anti-inflammatory activities on carageenan-induced mouse paw edema and nitric oxide (NO) production in LPSstimulated RAW264.7 macrophages (Wang et al 2008;Kou et al 2006;Kim et al 2009). Triterpenoid saponins from the roots of P. japonica Houtt.…”

Section: Discussionmentioning

confidence: 99%

Polygalasaponin F treats mice with pneumonia induced by influenza virus

Wang

Liu

et al. 2019

Inflammopharmacol

View full text Add to dashboard Cite

Background Influenza is an acute viral respiratory illness that causes high morbidity and mortality globally. Therapeutic actions are limited to vaccines and a few anti-viral drugs. Polygala (P.) japonica herba is rich in Polygalasaponin F (PSF, C 53 H 86 O 23), used for acute bronchitis, pharyngitis, pneumonia, amygdalitis, and respiratory tract infections treatment in China. Hypercytokinemia is often correlated with severe pneumonia caused by several influenza viruses. PSF was reported to have anti-inflammatory effects and its mechanism is associated with the nuclear factor (NF)-κB signaling pathway. The action of PSF to alleviate pulmonary inflammation caused by influenza A virus (IAV) infection requires careful assessment. In the present study, we evaluated the effect and mechanism of PSF on mice with pneumonia caused by influenza H1N1 (A/ FM/1/47). Methods Mice were infected intranasally with fifteen 50% mouse lethal challenge doses (MLD 50) of influenza virus. BALB/c mice were treated with PSF or oseltamivir (oral administration) for 2 h post-infection and received concomitant treatment for 5 days after infection. On day 6 post-infection, 10 mice per group were killed to collect related samples, measure body weight and lung wet weight, and detect the viral load, cytokine, prostaglandins, pathological changes, and cell pathway protein expression in the lungs. In addition, the survival experiments were carried out to investigate the survival of mice. The expression profile of cell pathway proteins was detected and analyzed using a broad pathway antibody array and confirmed the findings from the array by western blotting. Results Polygalasaponin F and oseltamivir can protect against influenza viral infection in mice. PSF and oseltamivir significantly relieved the signs and symptoms, reduced body weight loss, and improved the survival rate of H1N1-infected mice. Moreover, PSF efficiently decreased the level of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-4, interferon (IFN)-γ, thromboxane A 2 (TXA 2), and prostaglandin E 2 (PGE 2) in lung tissues of mice infected with influenza virus (p < 0.05-0.01). Oseltamivir had a similar effect to lung cytokine of PSF, but did not decrease the levels of TXA 2 and PGE 2. There was a twofold or greater increase in four cell pathway protein, namely NF-κB p65 (2.68-fold), I-kappa-B-alpha (IκBα) (2.56-fold), and MAPK/ERK kinase 1 (MEK1) (7.15-fold) assessed in the array induced by influenza virus. Western blotting showed that the expression of these proteins was significantly decreased in lung after influenza virus challenge in PSF and oseltamivirtreated mice (p < 0.05-0.01). Conclusion Polygalasaponin F appears to be able to augment protection against IAV infection in mice via attenuation of pulmonary inflammatory responses. Its effect on IAV-induced pulmonary inflammation was associated with suppression of Raf/MEK/ERK and NF-κB expressions.

show abstract

Chemical constituents isolated from Polygala japonica leaves and their inhibitory effect on nitric oxide production in vitro

Cited by 15 publications

References 18 publications

Kaempferol acetylated glycosides from the seed cake of Camellia oleifera

Kaempferol acetylated glycosides from the seed cake of Camellia oleifera

Membrane permeability-guided identification of neuroprotective components fromPolygonum cuspidatun

Polygalasaponin F treats mice with pneumonia induced by influenza virus

Contact Info

Product

Resources

About