Membrane permeability-guided identification of neuroprotective components from<i>Polygonum cuspidatun</i>

Xiao, Haitao; Qi, Xiaolan; Liang, Yan; Lin, Cheng Yuan; Wang, Xia; Guan, Zhi‐Zhong; Hao, Xiao‐Jiang

doi:10.3109/13880209.2013.837078

Cited by 14 publications

(11 citation statements)

References 20 publications

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“…The cells were cultured in 96-well microplates with a density of 1 × 10 4 cells/well and incubated with different concentrations of p -CA (0–100 μ M), respectively, with or without the exposure of H 2 O 2 . After 24 h incubation, the culture medium was removed and cell viability was measured using the MTT method as previously described [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

p‐Coumaric Acid Protects Human Lens Epithelial Cells against Oxidative Stress‐Induced Apoptosis by MAPK Signaling

Jiao

Zheng

Liang

et al. 2018

Oxidative Medicine and Cellular Longevity

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To protect against oxidative stress-induced apoptosis in lens epithelial cells is a potential strategy in preventing cataract formation. The present study aimed at studying the protective effect and underlying mechanisms of p-coumaric acid (p-CA) on hydrogen peroxide- (H2O2-) induced apoptosis in human lens epithelial (HLE) cells (SRA 01–04). Cells were pretreated with p-CA at a concentration of 3, 10, and 30 μM before the treatment of H2O2 (275 μM). Results showed that pretreatment with p-CA significantly protected against H2O2-induced cell death in a dose-dependent manner, as well as downregulating the expressions of both cleaved caspase-3 and cleaved caspase-9 in HLE cells. Moreover, p-CA also greatly suppressed H2O2-induced intracellular ROS production and mitochondrial membrane potential loss and elevated the activities of T-SOD, CAT, and GSH-Px of H2O2-treated cells. As well, in vitro study showed that p-CA also suppressed H2O2-induced phosphorylation of p-38, ERK, and JNK in HLE cells. These findings demonstrate that p-CA suppresses H2O2-induced HLE cell apoptosis through modulating MAPK signaling pathways and suggest that p-CA has a potential therapeutic role in the prevention of cataract.

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Section: Methodsmentioning

confidence: 99%

p‐Coumaric Acid Protects Human Lens Epithelial Cells against Oxidative Stress‐Induced Apoptosis by MAPK Signaling

Jiao

Zheng

Liang

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…SRA01/04 cells were cultured in 96-well plates at a density of 1×10 6 cells/well, as previously described (20). The cells were exposed to H 2 O 2 (Sigma) at different concentrations (0, 50, 100, 150, 200, 250, 300, 350, 400, or 500 μM) for 24 hours.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Long non-coding RNA nuclear paraspeckle assembly transcript 1 protects human lens epithelial cells against H2O2 stimuli through the nuclear factor kappa b/p65 and p38/mitogen-activated protein kinase axis

Zhou¹,

Yang²,

Zhang³

et al. 2020

Ann Transl Med

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Background: Long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) plays a regulatory role in many biological processes; however, its role in cataracts has yet to be illuminated. This study aimed to investigate the protective role of NEAT1 in hydrogen peroxide (H 2 O 2 )-treated human lens epithelial cells (HLECs) and its underlying molecular mechanism.Methods: HLECs (SRA01/04) were treated with 300 μM H 2 O 2 to mimic cataract in vitro. Cell viability was detected by performing an MTT assay and EdU staining. Flow cytometry was carried out to detect apoptosis of HLECs. DNA damage was examined using γ-H2A histone family member X staining. and reactive oxygen species (ROS) production was measured using 2',7'dichlorofluorescin diacetate staining. The expression levels of lncRNA and proteins were detected with quantitative real-time polymerase chain reaction and western blot, respectively.Results: The expression of NEAT1 was observed to be increased in H 2 O 2 -treated HLECs and agerelated cataract (ARC) tissues. Knockdown NEAT1 strongly protected against H 2 O 2 -induced cell death and also regulated the expression of cleaved caspase-3, B-cell lymphoma 2, and Bcl-2-associated X protein.Further, knockdown NEAT1 also significantly suppressed H 2 O 2 -induced intracellular ROS production and malondialdehyde (MDA) content, but elevated the glutathione (GSH) activity of H 2 O 2 -treated cells. Also, it is demonstrated that si-NEAT1 greatly inhibited H 2 O 2 -induced phosphorylation of NF-κB p65 and p38 MAPK.Conclusions: This study confirmed that knockdown NEAT1 attenuated H 2 O 2 -induced damage in HLECs, and inhibited the oxidative stress and apoptosis of HLECs via regulating nuclear factor-kappa B (NF-κB) p65 and p38 MAPK signaling. It may provide a potential target for clinical treatment of cataracts.

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“…It is used in the treatment of diseases, including asthma, atherosclerosis, cough, inflammation, hypertension, heart disease, fungal and bacterial infections and tumors [8]. Japanese knotweed also displays several beneficial biological effects such as inhibition of topoisomerases and neuraminidases, anti-oxidancy, antitumor activity, neuroprotective properties and inhibition of the development of borreliosis [3,[9][10][11]. Thanks to these properties, Japanese knotweed can be used as an alternative material of natural origin, which is a source of bioactive compounds in the prevention or treatment of many diseases.…”

Section: Introductionmentioning

confidence: 99%

UPLC-PDA-Q/TOF-MS identification of bioactive compounds and on-line UPLC-ABTS assay in Fallopia japonica Houtt and Fallopia sachalinensis (F.Schmidt) leaves and rhizomes grown in Poland

Lachowicz

Oszmiański

Cebulak

et al. 2018

Eur Food Res Technol

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The activity of polyphenolic compounds, triterpenoids, carotenoids, chlorophylls and antioxidants in leaves and rhizomes of Fallopia japonica Houtt and Fallopia sachalinensis (F.Schmidt) grown in Poland was investigated. Leaves and rhizomes were assessed for the presence of bioactive compounds with the ultra-performance liquid chromatography photodiode detector-quadrupole/time-of-flight mass spectrometry (UPLC-PDA-Q/TOF-MS) method, and for antioxidant activity with the on-line UPLC-ABTS screening. Forty-six polyphenolic compounds (15 phenolic acids, 12 flavones and flavonols, 11 flavan-3-ols and 8 stilbenes), were identified in Fallopia japonica and Fallopia sachalinensis. Furthermore, accurate mass measurement technique was for the first time in Fallopia japonica Houtt and Fallopia sachalinensis (F.Schmidt) in leaves and rhizomes it identified 25 new compounds belonging to carotenoids (9), chlorophylls (13) and triterpenoids (3) as well as rated the antioxidant properties of each polyphenolic compound. Major qualitative differences were found in the profiles. The leaves and rhizomes were found to be a good source not only of (average 20408.18 and 2716.42 mg/100 g dm), but also chlorophylls (average 179.97 and 43.82 mg/100 g dm), carotenoids (average 100.23 and 53.25 mg/100 g dm) and triterpenoids (average 580.87 and 434.05 mg/100 g dm). The content of bioactive compounds in Fallopia japonica Houtt was around 8.0, 4.0, 2.0 and 1.3 times higher than the content of polyphenols, chlorophylls, carotenoids and triterpenoids in Fallopia sachalinensis (F.Schmidt). The accurate identification of Fallopia bioactive compounds is an indispensable detailed knowledge of the profile and step toward better understanding of the medicinal properties of the species and also potentially more extensive use of the plant.

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Membrane permeability-guided identification of neuroprotective components fromPolygonum cuspidatun

Cited by 14 publications

References 20 publications

p‐Coumaric Acid Protects Human Lens Epithelial Cells against Oxidative Stress‐Induced Apoptosis by MAPK Signaling

p‐Coumaric Acid Protects Human Lens Epithelial Cells against Oxidative Stress‐Induced Apoptosis by MAPK Signaling

Long non-coding RNA nuclear paraspeckle assembly transcript 1 protects human lens epithelial cells against H2O2 stimuli through the nuclear factor kappa b/p65 and p38/mitogen-activated protein kinase axis

UPLC-PDA-Q/TOF-MS identification of bioactive compounds and on-line UPLC-ABTS assay in Fallopia japonica Houtt and Fallopia sachalinensis (F.Schmidt) leaves and rhizomes grown in Poland

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