Chemical contaminants and hepatic lesions in winter flounder (Pleuronectes americanus) from the northeast coast of the United States

Johnson, Lyndal L.; Stehr, Carla M.; Olson, O. Paul; Myers, Mark S.; Pierce, Susan M.; Wigren, Catherine A.; McCain, Bruce B.; Varanasi, Usha

doi:10.1021/es00049a015

Cited by 131 publications

(66 citation statements)

References 53 publications

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“…These authors, who suggested a relationship with environmental degradation for these lesions, considered the cells to be hepatocytes, although in our material the condition seemed to be limited to bile duct epithelium, as was reported and discussed by Moore et al (1989) for winter flounder and Stehr et al (1991) for rock sole Lepidopsetta bilineata and starry flounder Platichthys stellatus. Several authors have documented the association of this lesion with contaminant exposure in several species (Johnson et al 1993, Myers et al 1994, and it is generally considered a convenient histological biomarker of contaminant exposure. Although we did find focal hypervacuoiated nepaiocyies which were r~o i associated with the bile ducts, they showed different characteristics (Table 2) from the bile duct lesion, and were therefore classified separately.…”

Section: Focl Of Cellular Alteration ] Hydropic Vacljoliution Of Bilimentioning

confidence: 99%

Diseases of flounder Platichthys flesus in Dutch coastal and estuarine waters, with particular reference to environmental stress factors. II. Liver histopathology

Ad¹,

Pw²

1996

Dis. Aquat. Org.

126

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Diseases of flounder Platichthys flesus in Dutchcoastal and estuarine waters, with particular reference to environmental stress factors. were diagnosed as hepatocellular carcinoma. Most of the remaining nodules were diagnosed as foci of cellular alteration, whlch are considered to be preneoplastic lesions. Routine histopathological examination of livers showing no gross pathology resulted in the identification of a range of lesions including hepatocellular adenoma, foci of cellular alteration, hydropic vacuolization of biliary epithelial cells and hepatocytes, inflammatory lesions, focal necrosis, regenerative foci, and fibrillar hepatocytes of unknown significance. In addition, indices were used to quantify the presence of storage vacuoles (glycogen and lipid) and the relative density of melanomacrophage centres. The results indicate that a small proportion of neoplasms would be missed if only livers with grossly detectable nodules were subjected to histological examination. The prevalence of foci of cellular alteration at the different sampling sites showed a good correspondence with that of neoplasms, providing support for the hypothesis that these conditions represent stages of the same process. Of the other lesions and quantitative ~ndices, only hydropic vacuolization of billary epithelia] cells had a spatial distribution similar to that of neoplasms. 11 is concluded that liver neoplasms and especially their precursor lesions in flounder are promising tools for monitoring exposure to potential carcinogens, provided that migration patterns are explicitly taken into account. Furthermore, experimental studies are needed to investigate the chemical agents responsible for the onset of these early lesions and their progression into neoplasms, and also to evaluate the contribution of other environmental and host-related factors. Liver histopathology

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Section: Focl Of Cellular Alteration ] Hydropic Vacljoliution Of Bilimentioning

confidence: 99%

Diseases of flounder Platichthys flesus in Dutch coastal and estuarine waters, with particular reference to environmental stress factors. II. Liver histopathology

Ad¹,

Pw²

1996

Dis. Aquat. Org.

126

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“…Both of these cell types appear to be proliferative (Moore & Stegeman 1992, Koza et al 1993. In a survey of multiple sites on the U.S. East Coast, the prevalence of hydropic vacuolation was found to correlate closely with levels of polynuclear aromatic hydrocarbons and chlordanes in sediment and stomach contents in winter flounder, starry flounder and white croaker (Johnson et al 1992, Myers et al 1994. We have also shown that the first cell to undergo vacuolation IS a bihary preductular cell (Moore et al 1989).…”

Section: Discussionmentioning

confidence: 72%

“…In their 1984 sample Murchelano & Wolke (1991) also found that hepatic tumors in winter flounder from Boston Harbor wel-e predominantly cholangiocellular; our results thus establish that this general neoplastic phenotype is persistent over at least 7 yr. Cholangiocellular tumors have also predominated in some other bottom-feeding fish from contaminated sites, notably in the brown bullhead Ictalurus nebulosus (Baumann et al 1987, Hayes et al 1990) and the white sucker Catoston~us commersoni (Hayes et al 1990). In contrast, hepatocellular neoplasms were more common than cholangiocellular neoplasms in winter flounder from 10 U.S. East Coast sites (Johnson et al 1992), English sole Pleuronectes vetulus ) and the mummichog Fundulus heteroclitus (Vogelbein et al 1990). …”

Section: Discussionmentioning

confidence: 96%

“…Sediments in Boston Harbor contain high levels of polynuclear aromatic hydrocarbons, halogenated hydrocarbons, and heavy metals (Boehm 1984, Shiaris & Jambard-Sweet 1986, NOAA 1988, many of which are suspected carcinogens. Furthermore, a survey of chemical contaminants and winter flounder liver pathology at 22 stations showed chemical contaminants to correlate most strongly with prevalence of hydropic vacuolation (Johnson et al 1992). Although the condition and its chemical association have been described, there has been neither a systematic description of the diversity and relative frequency of the neoplasms present, nor an analysis of the degree of association between each neoplasm type, and hydropic vacuolation.…”

Section: Introductionmentioning

confidence: 99%

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Hepatic neoplasms in winter flounder Pleuronectes americanus from Boston Harbor, Massachusetts, USA

Moore¹,

Stegeman²

1994

Dis. Aquat. Org.

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Deer Island Flats, Boston Harbor, Massachusetts, USA, is a n area that is heavily contaminated with organic and inorganic chemicals. At this site winter flounder Pleuronectes americanus have been found to have a high prevalence of hepatic lesions including neoplasms. We describe here the relative frequency and histology of different tumor types in a sample of 60 neoplasms from 29 fish. Nlnety percent of tumors were cholangiocellular and ranged from cholangiomas to tubular, scirrhous, solid or cystic anaplastic cholangiocarcinomas. Hepatocellular tumors were also occasionally present (10%). The majority of tumors were closely associated with hydropically vacuolated cholangiocytes and hepatocytes, often being observed within grossly visible aggregations of vacuolated cells, and with vacuolated cells at their advancing margins. Vacuolated cells may be involved in the progression to neoplasia.

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“…Initial effects of metal pollution may be evident only at cellular or tissue levels before significant changes are identified in fish behavior or external appearance. Histological alterations for example have been examined for decades in fish tissues and organs in order to assess the effects of pollutants (Johnson et al 1993;Stentiford et al 2003;Au 2004). Various responses of enzymes have been also observed 1616 V.S.…”

Section: Introductionmentioning

confidence: 99%

Biomarkers in European perch (Perca fluviatilis) liver from a metal-contaminated dam lake

et al. 2014

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The present study was carried out in three seasons -spring, summer and autumn in Topolnitsa Dam Lake (Bulgaria) which has been subjected to continuous contamination with trace metals due to copper extraction in the area. We investigated the trace metal levels in surface water and liver samples of European perch (Perca fluviatilis L.). We also linked the metal levels we determined with the various histological and biochemical changes which we observed. Lesions in the liver parenchyma were found to be degenerative and necrotic, as well as, they were presented as hyperemia which consequently leads to disturbances in the hepatic blood circulation. Activities of the hepatic enzymes lactate dehydrogenase (LDH), alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) were found to be significantly elevated, particularly in summer. Therefore, based on our results we could recommend that the investigated tissue and cell alterations may be successfully applied as reliable biomarkers for monitoring polluted with a mixture of trace metals freshwater ecosystems.

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Chemical contaminants and hepatic lesions in winter flounder (Pleuronectes americanus) from the northeast coast of the United States

Cited by 131 publications

References 53 publications

Diseases of flounder Platichthys flesus in Dutch coastal and estuarine waters, with particular reference to environmental stress factors. II. Liver histopathology

Diseases of flounder Platichthys flesus in Dutch coastal and estuarine waters, with particular reference to environmental stress factors. II. Liver histopathology

Hepatic neoplasms in winter flounder Pleuronectes americanus from Boston Harbor, Massachusetts, USA

Biomarkers in European perch (Perca fluviatilis) liver from a metal-contaminated dam lake

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