Chemical Content Profile of Essential Oil from Kaffir Lime (<i>Citrus hystrix</i> DC.) in Tanah Datar Regency and Antibacterial Activity

Husni, Elidahanum; Putri, Utari Septiana; Hamidi, Dachriyanus

doi:10.2991/ahsr.k.211105.025

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…The first highest peak was b-phellandrene, then b-pinene, d-limonene, and the fourth peak was citronella. This was in accordance with previous study conducted by Bustamante et al and Husni et al Both studies was conducted on sweet orange peel using the MAHD extraction method with water as solvent and kaffir lime peel using the HD extraction method with water as solvent [24,25]. The complete essential oil composition can be seen in Tables 1 and 2, respectively for variations in power and ratio of material to solvent.…”

Section: Resultssupporting

confidence: 90%

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Kinetics of Essential Oil Extraction of Kaffir Lime Peel Using Microwave Assisted Hydrodistillation

Megawati,

Triwibowo,

Bahlawan

et al. 2023

JBBBE

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The purpose of this study is to study the kinetics of kaffir lime peel essential oil extraction using microwave assisted hydrodistillation. Extraction was carried out at various powers and the ratios of material to solvent for 1 hour. Soxhlet extraction using n-hexane solvent was also carried out to determine the yield of essential oil. The extraction kinetics was investigated using a mass transfer-controlled approach and expressed by the 1st and 2nd order of reaction rate. The Soxhlet extraction results indicated that the yield of kaffir lime peel essential oil was 5.65% w/w. The higher the microwave power being applied (180, 300, 450, and 600 Watt), the higher the essential oil yielded (2.2; 2.8; 3; and 3.8% w/w), respectively, but at 800 Watt, the yield decreased (3.2% w/w). The smaller the ratio of material to solvent, which means more diluted, the lower the yield of oil were, namely from 4.56; 3.9; and 3.8% w/w, at ratios of material to solvent 1:3, 1:3.5, and 1:4 w/v. Different results were obtained at a ratio of 1:3 (4.56% w/w) which produced a yield greater than the ratio of 1:1.25 (2.88% w/w). Therefore, the optimum conditions for extracting kaffir lime peel essential oil were at 600 Watt and a ratio of material to solvent 1:3 w/w for 56 minutes with a yield of 4.58% and a density of 0.86 g/cm3. The kinetics of the 2nd order of homogeneous model better represented results of the experiments with extraction rate constants at 180, 300, 450, 600, and 800 Watt of 14.89; 14.95; 15.53; 21.32; and 19.85 L.g-1.min-1. While the extraction rate constants at the material to solvent ratio of 1:2.5; 1:3; 1:3.5; and 1:4 w/v of 17.38; 19.80; 22.09; and 32.31 L.g-1.min-1. The extraction capacity was also affected by the power and the ratio of material to solvent, the extraction capacity were 0.0095; 0.0100; 0.0104; 0.0125; and 0.0106 g.L-1, at 180, 300, 450, 600, and 800 Watt respectively, and 0.0094; 0.0134; 0.0134; and 0.0118 g.L-1, at material to solvent ratio of 1:2.5; 1:3; 1:3,5; and 1:4 w/v respectively.

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Section: Resultssupporting

confidence: 90%

“…Extraction of kaffir lime peel essential oil using MAE conducted by Lubinska-Szczygeł also produced essential oil with 30 components [1]. Similarly, Husni et al who extracted kaffir lime peel essential oil using HD, the quantity of detected components was 31 [25].…”

Section: Resultsmentioning

confidence: 92%

Kinetics of Essential Oil Extraction of Kaffir Lime Peel Using Microwave Assisted Hydrodistillation

Megawati,

Triwibowo,

Bahlawan

et al. 2023

JBBBE

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“…Furthermore, a study by Gao et al (2020) on 6-Octenal, 3,7-dimethyl-, (R)-(citronella) as a major compound of C. hystrix reported inhibiting bacteria such as S. aureus, S. mutans, E. coli, and P. aeruginosa. Citronella compound is an aldehyde functional group known as an antibacterial (Husni et al 2021).…”

Section: Resultsmentioning

confidence: 99%

Potential antibacterial and antioxidant activities of ten essential oils from East Kalimantan, Indonesia

Aryani

Kusuma

MELIANA³

et al. 2023

Biodiversitas

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Abstract. Aryani F, Kusuma IW, Meliana Y, Sari NM, Kuspradini H. 2023. Potential antibacterial and antioxidant activities of ten essential oils from East Kalimantan, Indonesia. Biodiversitas 24: 665-672. This study aimed to evaluate ten aromatic essential oils' antibacterial and antioxidant activity. Ten aromatic herbs were distilled using the water and steam distillation method. The essential oils obtained was tested for its physical properties, color, refractive index, and specific gravity. The refractive index was measured using a refractometer. On the other hand, specific gravity was measured using a pycnometer. Antioxidant activity testing using the free radical DPPH (2,2-diphenyl-1-picrylhydrazyl) and ascorbic acid was used as the control. Antibacterial activity testing using the agar diffusion method and pathogenic bacteria, namely Salmonella typhi, Escherichia coli, Propionibacterium acnes, and Staphylococcus aureus. Chloramphenicol was used as a positive control and acetone as a negative control. The results obtained essential oils with a slightly yellowish to brownish-yellow color. The highest refractive index and specific gravities were Syzygium aromaticum oil, and the smallest was Litsea elliptica and Eucalyptus pellita oils. The results of the antioxidant activity test carried out at a concentration of 500 µg/mL range from 4.82 % to 85.06 % inhibition and showed that the highest inhibition was Cymbopogon citratus and Syzygium aromaticum by 55.76 % and 85.06%, respectively. Antibacterial activity of ten essential oils showed a varied inhibition zone (0-60 mm) against S. typhi, E. coli, P. acnes, and S. aureus. The oil from Cymbopogon citratus and Citrus hystrix had the highest activity against S. typhi (60 and 21.67 mm, respectively), E. coli (60 and 20.33 mm, respectively), P. acnes (60 and 31.11 mm, respectively) and S. aureus (60 mm and 17.89 mm, respectively). The research results showed that the essential oils in this study had the potency for development as a natural antibacterial and antioxidants agents.

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“…According to another study, the kaffir lime peel EO has three primary chemical components: D-limonene (17.10%), 3-carene (13.77%), and γ-terpinene (12.56%). All three chemicals belong to the monoterpene hydrocarbon group and are characterized by the presence of double bonds, which are known as alkenes [42].…”

Section: Discussionmentioning

confidence: 99%

Chemometric Analysis of GC-MS Chemical Profiles and Biological Activities of Three Citrus Essential Oils in Indonesia

Nuryandani,

Novitasari,

Abdurrahman

et al. 2024

Preprint

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Citrus species essential oils (EOs) are significant sources of bioactive compounds and demonstrate diverse therapeutic effects. However, limited documentation exists regarding the phytochemicals associated with their biological activities. This study aims to assess the antioxidant activity, plant inhibitory properties, and brine shrimp cytotoxic effects of EOs derived from three Citrus species: C. sinensis, C. limon, and C. hystrix. Utilizing chemometric analysis and gas chromatography-mass spectrometry (GC-MS) fingerprints, the volatile components contributing to antioxidant activity were elucidated. The peels of the Citrus samples were hydro-distillated to obtain EOs, and subsequently subjected to antioxidant, plant inhibitory, and brine shrimp cytotoxic assays. The results indicated that C. limon EO exhibited the highest antioxidant activity, while C. hystrix and C. sinensis EOs demonstrated pronounced inhibitory effects against Aetrmia salina and Lactuca sativa, respectively. GC-MS analysis facilitated the identification of key compounds in each EO. Principal component analysis (PCA) and hierarchy cluster analysis (HCA) effectively categorized Citrus EOs based on their antioxidant properties, highlighting the proximity of C. limon and C. sinensis. Among the identified compounds, D-limonene, α-Terpineol, Caryophyllene, (+)-3-Carene, β-Pinene, (-)-Spathulenol, trans-p-Mentha-1(7),8-dien-2-ol, and trans-Verbenol were the most discriminating compounds affected the antioxidant activity of C.limon and C. sinensis EOs.

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Chemical Content Profile of Essential Oil from Kaffir Lime (Citrus hystrix DC.) in Tanah Datar Regency and Antibacterial Activity

Cited by 9 publications

References 15 publications

Kinetics of Essential Oil Extraction of Kaffir Lime Peel Using Microwave Assisted Hydrodistillation

Kinetics of Essential Oil Extraction of Kaffir Lime Peel Using Microwave Assisted Hydrodistillation

Potential antibacterial and antioxidant activities of ten essential oils from East Kalimantan, Indonesia

Chemometric Analysis of GC-MS Chemical Profiles and Biological Activities of Three Citrus Essential Oils in Indonesia

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