Chemical modification of protein a chromatography ligands with polyethylene glycol. II: Effects on resin robustness and process selectivity

Protein A chromatography: Challenges and progress in the purification of monoclonal antibodies

“…Years later, Weinberg et al. demonstrated the robustness and process selectivity of PEGylated resins. Upon digestion with chymotripsin, the resin with PEGylated ligands retained higher static BC compared to the unmodified trypsin.…”

Sustainable strategies based on glycine–betaine analogue ionic liquids for the recovery of monoclonal antibodies from cell culture supernatants

“…9 However, it presents several limitations, namely the low pH values necessary to elute mAb-products that can cause their aggregation, the presence of some impurities in the final formulation since the ProA ligand can be degraded by proteases present in the supernatants, and the high cost of the resin (between US$5,000 and US$15,000/L; industrial columns require volumes up to 1000 L). 9,10 Accordingly, ProA affinity chromatography is the main centre of costs of the mAbs manufacturing process, with the overall downstream processing contributing up to 80% of the mAbs global production costs. 1,11 Therefore, it is imperative to develop sustainable and cost-effective downstream processes for mAbs to decrease their cost and to allow their widespread use.…”

Synthesis and Applications of Polymer Chelating Resins Containing Schiff Base

“…This can be performed by a batch process or through an extraction column, in which the resin is supported unto silica gel, or glass support [8,9]. The selectivity of these resins depends mainly on the nature of ligand (antenna) within the resin backbone and pH values of the surrounded medium [10,11]. The uses of the chelating polymers can be summarized as (a) used as protective coatings on the metal's surfaces [12]; (b) utilized in manufacturing the heat resistant fibers used in the semiconductors [13]; (c) used as a membrane in the manufacturing of ionic selective electrodes [14]; (d) can be applied as a green analytical reagent, specifically, involves separating and concentrating of the copper ions from the aqueous solutions, by using the silica gel supported to aurintricarboxylic acid [15]; and (e) used as catalysts for specific chemical reactions such as oxidation, hydrogenation, hydrolysis and as polymeric initiators [16].…”