Chemical Modification of Transducin with Dansyl Chloride Hinders Its Binding to Light-activated Rhodopsin

Kosoy, Ana; Möller, Carolina; Perdomo, Deisy; Bubis, José

doi:10.5483/bmbrep.2004.37.2.260

Cited by 9 publications

(3 citation statements)

References 52 publications

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“…2). Maximum absorption wavelength and molar absorption coefficient for Dansyl-Cl in ACN solvent was 327 nm (e: 4.3610 2 L/ mol/cm/) [45,46]. Obviously, EASC exhibited strong UV absorption; it was attributed to the core structure of EASC, in which its p-p and n-p conjugation systems became strong due to the existing three fused heterocyclic aromatic rings compared with Dansyl-Cl which had The apparent dissociation constant pK a l 95% confidence limits was estimated using the ACD/pK a DB software as described in Section 2.…”

Section: Ultraviolet Absorption Of Eascmentioning

confidence: 99%

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

You

Zhao

Sun

et al. 2009

J of Separation Science

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Original PaperApplication of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS A simple, sensitive method for the determination of aliphatic amines based on a sulfonylation reaction using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as pre-column labeling reagent with fluorescence detection and APCI-MS identification has been developed. The labeled derivatives exhibited high stability and were enough to be efficiently analyzed by HPLC with an excitation maximum at k ex 270 nm and an emission maximum at k em 430 nm. Identification of derivatives was carried out by online post-column MS in positive-ion mode. Comparing with the widely used 5-dimethylaminonaphthalene-1-sulfonylchloride (Dansyl-Cl), EASC-amine derivatives not only exhibited high fluorescence but also exhibited excellent MS ionizable potential. Detection limits obtained from 0.10 pmol injection, at a S/N of 3, were 4.0 -12.7 fmol. The mean intra-and inter-assay precision for all aliphatic amine levels were a3.84 and 3.21%, respectively. Excellent linear responses were observed with coefficients of A0.9995.

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Section: Ultraviolet Absorption Of Eascmentioning

confidence: 99%

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

You

Zhao

Sun

et al. 2009

J of Separation Science

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“…The chemical modification technique that reveals the protein-protein interaction has been studied [16][17][18][19][20]. In many cases, the modified residues play an important role in the interaction, because they are located at the protrusion of the protein and have low pKa values in positively charged residues.…”

Section: Introductionmentioning

confidence: 99%

Role of positive charge of lysine residue on cytochrome c₃for electrostatic interaction with hydrogenase

Iida

Asakura

Tabata

et al. 2007

J. Porphyrins Phthalocyanines

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Cytochrome c 3 from Desulfovibrio vulgaris (Miyazaki) is an electron transfer protein containing four hemes per molecule. Its physiological electron transfer partner is the hydrogenase which catalyzes reversible oxidation of hydrogen. The complex formation between cytochrome c 3 and hydrogenase is caused by electrostatic interaction, because cytochrome c 3 is a basic protein and hydrogenase is an acidic protein. As cytochrome c 3 has 20 lysine residues among 108 amino acids, the positive charges of some lysine residues may play an important role in the interaction with hydrogenase. To clarify the role of positive charge of lysine residue, the positive charge was changed to neutral or negative charge using chemical modification and site-directed mutagenesis. When the positive charges around heme IV were changed, the hydrogen evolution rate with hydrogenase decreased. The affinity between hydrogenase and mutated cytochrome c 3 (K57Q, K57E, K72Q, K94Q, K94E) were not affected. On the other hand, the affinity of K72E cytochrome c 3 for hydrogenase was very low. These results suggest that the positive charge around heme IV plays an important role in the electrostatic interaction with hydrogenase in hydrogen evolution.

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“…Compared to other fluorophores, the azido-Ndansylethylamine fluorophore has a comparably low molar extinction coefficient of 3400 M -1 cm -1 . [204] The abolition of the PET quenching upon Zn(II) addition increased its fluorescence intensity by 40%. In contrast to other fluorescence metal sensors this is a rather small increase, which might be attributed to the relatively long spacer.…”

Section: Discussionmentioning

confidence: 99%

Development of Zinc-Finger-Based Artificial Restriction Endonucleases and Fluorescent Peptidyl Metal Sensors

Czerny¹

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With more than thousand different members that have been discovered yet, zinc finger proteins are considered to be one of the most comprehensive classes of DNA-binding proteins found in nature. [9] This is due to their participation as DNA-binding domains in the modular structure of many eukaryotic and prokaryotic transcription factors. In this function, they are involved in the early steps of DNA transcription, an ubiquitous process that uses the genetic information by translating DNA sequences into corresponding messenger RNAs (mRNAs). Thereby, ZFPs recognize a specific promoter sequence, which is located upstream of the genetic code that should be transcribed by RNA-polymerases (Figure 1.1).Under retention of an uniform globular -structure, zinc fingers differ in their individual amino acid sequences to bind a large variety of promoter regions in a sequence-specific manner. This explains the high diversity of this protein family in the cellular environment and finally allows them to trigger transcriptional processes for a myriad of genes.

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Chemical Modification of Transducin with Dansyl Chloride Hinders Its Binding to Light-activated Rhodopsin

Abstract: Transducin (T),

Cited by 9 publications

References 52 publications

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

Role of positive charge of lysine residue on cytochrome c₃for electrostatic interaction with hydrogenase

Development of Zinc-Finger-Based Artificial Restriction Endonucleases and Fluorescent Peptidyl Metal Sensors

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Chemical Modification of Transducin with Dansyl Chloride Hinders Its Binding to Light-activated Rhodopsin

Abstract: Transducin (T),

Cited by 9 publications

References 52 publications

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

Role of positive charge of lysine residue on cytochrome c3for electrostatic interaction with hydrogenase

Development of Zinc-Finger-Based Artificial Restriction Endonucleases and Fluorescent Peptidyl Metal Sensors

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Product

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Role of positive charge of lysine residue on cytochrome c₃for electrostatic interaction with hydrogenase