Chemical mutagenesis of the mouse genome: an overview

Guénet, Jean-Louis

doi:10.1007/978-1-4020-5057-2_2

Cited by 19 publications

(13 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, a number of studies have been con ducted for creating animal models of human dis eases by chemical mutagenesis (e. g., Nethyl ni trosourea; ENU) that causes random allelic point mutations in mice. However, the major limitation of animal models is the phenotype that often does not reflect human beings (62,63).…”

Section: Functional Genomics Integrating Model Systemsmentioning

confidence: 99%

The most common technologies and tools for functional genome analysis

Gasperskaja¹,

Kučinskas²

2017

AML

View full text Add to dashboard Cite

Since the sequence of the human genome is complete, the main issue is how to understand the information written in the DNA se quence. Despite numerous genomewide studies that have already been performed, the challenge to determine the function of genes, gene products, and also their interaction is still open. As changes in the human genome are highly likely to cause pathological con ditions, functional analysis is vitally important for human health.For many years there have been a variety of technologies and tools used in functional genome analysis. However, only in the past decade there has been rapid revolutionizing progress and improvement in highthroughput methods, which are rang ing from traditional realtime polymerase chain reaction to more complex systems, such as nextgeneration sequencing or mass spectrometry. Furthermore, not only laboratory investigation, but also accurate bioinformatic analysis is required for reliable scientific results. These methods give an opportunity for accu rate and comprehensive functional analysis that involves various fields of studies: genomics, epigenomics, proteomics, and inter actomics. This is essential for filling the gaps in the knowledge about dynamic biological processes at both cellular and organis mal level. However, each method has both advantages and limita tions that should be taken into account before choosing the right method for particular research in order to ensure successful study. For this reason, the present review paper aims to describe the most frequent and widelyused methods for the comprehen sive functional analysis.

show abstract

Section: Functional Genomics Integrating Model Systemsmentioning

confidence: 99%

The most common technologies and tools for functional genome analysis

Gasperskaja¹,

Kučinskas²

2017

AML

View full text Add to dashboard Cite

show abstract

“…ENU induces point mutations (mostly base pair substitutions) and increases the spontaneous mutation rate by a factor of 130 to 160 depending on the experimental conditions (Guénet 2004). Now, at least twelve ENU projects are in progress over the five continents and thousands of mice, offspring of mutagenized progenitors and potentially affected by mutations (dominant or recessive), are carefully analyzed each year for a large number of phenotypic criteria.…”

Section: Inducing Mutations With Chemicalsmentioning

confidence: 99%

The mouse genome

Guénet¹

2005

Genome Res.

View full text Add to dashboard Cite

The house mouse has been used as a privileged model organism since the early days of genetics, and the numerous experiments made with this small mammal have regularly contributed to enrich our knowledge of mammalian biology and pathology, ranging from embryonic development to metabolic disease, histocompatibility, immunology, behavior, and cancer. Over the past two decades, a number of large-scale integrated and concerted projects have been undertaken that will probably open a new era in the genetics of the species. The sequencing of the genome, which will allow researchers to make comparisons with other mammals and identify regions conserved by evolution, is probably the most important project, but many other initiatives, such as the massive production of point or chromosomal mutations associated with comprehensive and standardized phenotyping of the mutant phenotypes, will help annotation of the ∼25,000 genes packed in the mouse genome. In the same way, and as another consequence of the sequencing, the discovery of many single nucleotide polymorphisms and the development of new tools and resources, like the Collaborative Cross, will contribute to the development of modern quantitative genetics. It is clear that mouse genetics has changed dramatically over the last 10-15 years and its future looks promising.

show abstract

“…Although not a new technique, chemical mutagenesis of mice recently experienced a renaissance as a method for functional genome analysis on a large scale (105)(106)(107)(108). The chemical of choice is ethylnitrosourea (ENU), an alkylating agent that causes mutation at a frequency of 300-1,200 times greater than the spontaneous mutation rate (109).…”

Section: Ethylnitrosourea Mutagenesismentioning

confidence: 99%

Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders Approaches to lipid metabolism gene identification and characterization in the postgenomic era

Reue¹,

Vergnes²

2006

Journal of Lipid Research

View full text Add to dashboard Cite

The availability of genomic resources has already had a tremendous impact on biomedical research. In this review, we describe how whole genome sequence and highthroughput functional genomics projects have facilitated the identification and characterization of important genes in lipid metabolism and disease. We review key approaches and lipid genes identified in the first years of this century and discuss how genomic resources are likely to streamline gene identification and functional characterization in the future.-Reue, K., and L. Vergnes. Approaches to lipid metabolism gene identification and characterization in the postgenomic era.

show abstract

Chemical mutagenesis of the mouse genome: an overview

Cited by 19 publications

References 72 publications

The most common technologies and tools for functional genome analysis

The most common technologies and tools for functional genome analysis

The mouse genome

Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders Approaches to lipid metabolism gene identification and characterization in the postgenomic era

Contact Info

Product

Resources

About