Chemical Mutation Induction in vitro Cultured Shoot Tip of Banana Cv. Grand Nain for Resistance some Virus Diseases

Sayed, El; Eman, H.; Mahfouze, Sherin A.; Shaltout, A. D.; Dougdo, Kh.A. El; Sayed, R. A.

doi:10.3923/ijv.2012.178.190

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…Wannajindaporn et al (2014) reported that 28 Dendroium'Earsakul'mutants were obtained with protocorm-like bodies using 0-5 mM NaN3 under in vitro conditions. Abiotic and biotic stresstolerant mutants have been obtained by applying chemical mutagens coupled with in vitro systems in chrysanthemum (salt), sugarcane (salt), banana (drought) and banana (viruses) (Hossain et al 2006;Ikram-ul-Haq et al 2011;Bidabadi et al 2011;El-Sayed et al 2012). The general strategy in breeding is that segregating the offspring, whose genomes have been modified such a kind of techniques mutagen applications or hybridizations, at least six generations are needed to fix their modified genomes.…”

Section: Discussionmentioning

confidence: 99%

Obtaining Candidate Salt Tolerant Wheat Mutant Lines Derived From Combination of Sodium Azide Mutagenesis and Somatic Embryogenesis

Şen

Sarsu

2019

Trakya University Journal of Natural Sciences

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Plant mutants are important bio-resources for crop breeding and functional gene studies. In the present study, conventional chemical mutagenesis technique was combined with somatic embryogenesis to obtain candidate salt tolerant mutant wheat lines. For this purpose, 0-5 mM Sodium Azide (NaN3) was applied for 30 minutes to embryonic calli under in vitro conditions to produce genetic variations in the bread wheat (Triticum aestivum L. cv. Adana 99). Treated and non-treated calli were put in somatic embryo induction media, and 3 and 4 mM NaN3 were determined as optimum mutation doses for somatic embryo induction. The obtained somatic embryos from these optimum mutagen doses were then screened for tolerance in regeneration media containing 125 mM NaCl to be used to improve tolerance to salt stress. In NaN3 treatment, 14 mutants with moderate salt tolerance were obtained. The results suggest that the in vitro technique in combination with chemical mutagenesis may be a useful approach for accelerating breeding strategies to create enough genetic variation in populations and to get fourth generation putative salt tolerant wheat mutant lines in less than 1.5 years.

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Section: Discussionmentioning

confidence: 99%

Obtaining Candidate Salt Tolerant Wheat Mutant Lines Derived From Combination of Sodium Azide Mutagenesis and Somatic Embryogenesis

Şen

Sarsu

2019

Trakya University Journal of Natural Sciences

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“…Banana bunchy top virus (BBTV) is a highly infectious and destructive virus of the banana plantation. Furthermore, the disease can severely reduce the quality and marketability of fruit, resulting in huge economic losses when left uncontrolled (Rybicki and Pietersen 1999;Eman et al, 2012 andRybicki, 2015). Banana bunchy top disease is characterized by dark green streaks on the lower portions of the midrib of the leaf and the fresh infected leaves were stiff and erect, shorter and narrow with brittle, yellow and wavy edges (Allam et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The Incidence and Molecular Characterization of Banana Bunchy Top Virus (BBTV) Infecting Banana (Musa spp.) in Ismailia governorate, Egypt

Amin,,

Farag,

Abouelwafa

2023

Egyptian Academic Journal of Biological Sciences. C, Physiology

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The banana is the most important fruit in Egypt. Banana bunchy top disease, caused by the banana bunchy top virus (BBTV), is one of the most damaging infections on banana plantations, resulting in significant crop losses. The current study was conducted to characterize the incidence of banana bunchy top disease (BBTD) and to study the molecular characteristics of BBTV from banana plantations in Ismailia. This study was conducted during the period 2019-2021 in three locations (Abu-Suwayr, El Tal-El kabir and Fayed) in Ismailia Governorate. The occurrence of BBTV was initially studied using visual inspection of external virus symptoms. TAS-ELISA, PCR and nucleic acid hybridization were used to detect the virus. Further molecular characterization of BBTV was performed based on Rolling Circle Amplification (RCA) and nucleotide sequencing. BBTD incidences ranging from 3.33 to 5.14% were observed in two localities Abu-Suwayr and El Tal-El kabir. While a relatively low incidence of 1.48% was observed in Fayed. Our results showed that the BBTD incidence is directly related to the percentage of winged aphids inside the aphid colonies regardless the percentage of the aphid's occurrence. The PCR product (973 bp) of BBTV-component 3 (DNA-S) was used for the creation of a non-radioactive Dig labeled-DNA probe for further use for BBTV detection using dot blot and Southern blot hybridization. The sequence comparison analysis of DNA-S component showed that the present Ismailia isolate (BBTV-3F) shared nucleotide identity 98.4%-91.9 % with three isolates from Qalubya Egypt and shared 97.9% nt identity with east African isolates (Rwanda and Malawi). The phylogenetic analysis revealed that the DNA-component 3 (DNA-S) of the BBTV-3F isolate from Ismailia belongs to the South Pacific group (SPG) and is markedly separated from those of the Asian group (AG). This study is important for monitoring and early detection of BBTV threats and for rapid eradication of infected plants before the virus spreads and settles in a new area.

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“…A critical mining of literature indicates that very few studies have been performed against PGs of insects and bacteria till date (26) and interestingly, there is no report of inhibition or resistance achieved against bacterial prominent soft rot pathogen like Erwinia spp. PGIP protein has been found very promising in increase in breeding efficiency of banana against various biotic stresses have been reported by radiation mutagenesis (27,28), chemical mutagenesis (29,30) and genome editing (31).…”

Section: Introductionmentioning

confidence: 99%

An insight into molecular interaction of PGIP with PG for banana cultivar

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Chemical Mutation Induction in vitro Cultured Shoot Tip of Banana Cv. Grand Nain for Resistance some Virus Diseases

Cited by 11 publications

References 10 publications

Obtaining Candidate Salt Tolerant Wheat Mutant Lines Derived From Combination of Sodium Azide Mutagenesis and Somatic Embryogenesis

Obtaining Candidate Salt Tolerant Wheat Mutant Lines Derived From Combination of Sodium Azide Mutagenesis and Somatic Embryogenesis

The Incidence and Molecular Characterization of Banana Bunchy Top Virus (BBTV) Infecting Banana (Musa spp.) in Ismailia governorate, Egypt

An insight into molecular interaction of PGIP with PG for banana cultivar

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