Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom

Tanuwidjaja, Irina; Svečnjak, Lidija; Gugić, Domenika; Levanić, Marko; Jurić, Slaven; Vinceković, Marko; Fuka, Mirna Mrkonjić

doi:10.3390/molecules26103049

Cited by 18 publications

(12 citation statements)

References 43 publications

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“…On the other hand, the MIC values for S. aureus were higher than what was demonstrated 30,31 . Similarly, the MIC values of AmBV were 15.625 µg mLG 1 for E. coli ATCC8739 and 7.8 µg mLG 1 for both S. marcescens AUH 98 and S. mutans ATCC 25175 which was much lower than the previously reported MIC values 9,[32][33][34][35] .…”

Section: Discussioncontrasting

confidence: 57%

Apis mellifera Venom Inhibits Bacterial and Fungal Pathogens in vitro

Bakhiet¹,

Hussi²,

Elshehaby³

2022

Pakistan J. of Biological Sciences

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Background and Objective: Bacterial and fungal infections are major public health problems. Emerging of drug-resistant microbial strains urges the need for the development of alternative untraditional antimicrobial agents. Bee venom is a rich source of secondary metabolites and antimicrobial agents. In this study, the antimicrobial and antifungal potential of Apis mellifera BV (AmBV) against some medically important bacterial and fungal pathogens was investigated. Materials and Methods: Broth microdilution method and Colony Forming Unit (CFU) assay were used to screen the antibacterial potential of AmBV. Similarly, the antifungal activity of AmBV was evaluated using the agar-well diffusion assay. Moreover, the minimum inhibitory concentration (MIC) values of AmBV against tested microorganisms were determined. Results: AmBV significantly inhibited bacterial and fungal growth.

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Section: Discussioncontrasting

confidence: 57%

Apis mellifera Venom Inhibits Bacterial and Fungal Pathogens in vitro

Bakhiet¹,

Hussi²,

Elshehaby³

2022

Pakistan J. of Biological Sciences

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“…For the determination of melittin in BV, other authors have also used a C18 HPLC column, such as the study recently published by Tanuwidjaja et al (2021) [ 24 ]. In our study, the analysis was carried out following the methods described by Zhou et al (2010) [ 25 ], Dong et al (2015) [ 26 ] and Pereira et al (2016) [ 12 ], with modifications ( Section 2.1 ).…”

Section: Resultsmentioning

confidence: 99%

Africanized Bee Venom (Apis mellifera Linnaeus): Neuroprotective Effects in a Parkinson’s Disease Mouse Model Induced by 6-hydroxydopamine

Dantas

Paixão

Nunes

et al. 2022

Toxics

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This study evaluated the neuroprotective effects of the Africanized bee venom (BV) and its mechanisms of action after 6-hydroxydopamine-(6-OHDA)-induced lesion in a mice model. Prior to BV treatment, mice received intrastriatal microinjections of 6-OHDA (no induced dopaminergic neuronal death) or ascorbate saline (as a control). BV was administered subcutaneously at different dosages (0.01, 0.05 or 0.1 mg·Kg−1) once every two days over a period of 3 weeks. The open field test was carried out, together with the immunohistochemical and histopathological analysis. The chemical composition of BV was also assessed, identifying the highest concentrations of apamin, phospholipase A2 and melittin. In the behavioral evaluation, the BV (0.1 mg·Kg−1) counteracted the 6-OHDA-induced decrease in crossings and rearing. 6-OHDA caused loss of dopaminergic cell bodies in the substantia nigra pars compacta and fibers in striatum (STR). Mice that received 0.01 mg·Kg−1 showed significant increase in the mean survival of dopaminergic cell bodies. Increased astrocytic infiltration occurred in the STR of 6-OHDA injected mice, differently from those of the groups treated with BV. The results suggested that Africanized BV has neuroprotective activity in an animal model of Parkinson’s disease.

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“…The bioactive components present in the venom have generated much interest in medicine through the different species of the Apis genus, and their application in in vitro antimicrobial activity [ 67 ], their cytotoxic action against cancer cells [ 68 ], even the synergistic effect of the venom with some antibiotics such as Cephotax, Cefepime, and Tavanic has been revealed [ 69 ]. Through Transmission Electron Microscopy, the deformation of the cell wall was appreciated, resulting in the destruction of the cell wall, changes in the permeability of the membrane, leakage of cell content, inactivation of metabolic activity, and finally, cell death [ 57 ], as the inhibitory effect on F1-F0—ATPase has also been demonstrated [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Apis mellifera Bee Venom Collected in Northern Peru

2023

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Due to the emergence of microorganisms resistant to antibiotics and the failure of antibiotic therapies, there is an urgent need to search for new therapeutic options, as well as new molecules with antimicrobial potential. The objective of the present study was to evaluate the in vitro antibacterial activity of Apis mellifera venom collected in the beekeeping areas of the city of Lambayeque in northern Peru against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Bee venom extraction was performed by electrical impulses and separated using the Amicon ultra centrifugal filter. Subsequently, the fractions were quantified by spectrometric 280 nm and evaluated under denaturant conditions in SDS-PAGE. The fractions were pitted against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853. A purified fraction (PF) of the venom of A. mellifera and three low molecular weight bands of 7 KDa, 6 KDa, and 5 KDa were identified that showed activity against E. coli with a MIC of 6.88 µg/mL, while for P. aeruginosa and S. aureus, it did not present a MIC. No hemolytic activity at a concentration lower than 15.6 µg/mL and no antioxidant activity. The venom of A. mellifera contains a potential presence of peptides and a predilection of antibacterial activity against E. coli.

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Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom

Cited by 18 publications

References 43 publications

Apis mellifera Venom Inhibits Bacterial and Fungal Pathogens in vitro

Apis mellifera Venom Inhibits Bacterial and Fungal Pathogens in vitro

Africanized Bee Venom (Apis mellifera Linnaeus): Neuroprotective Effects in a Parkinson’s Disease Mouse Model Induced by 6-hydroxydopamine

Antimicrobial Activity of Apis mellifera Bee Venom Collected in Northern Peru

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