Chemical profiling of Phlomis thapsoides (Lamiaceae) and in vitro testing of its biological activities

Sobeh, Mansour; Mamadalieva, Nilufar Z.; Mohamed, Tamer; Krstin, Sonja; Youssef, Fadia S.; Ashour, Mohamed L.; Azimova, Sh. S.

doi:10.1007/s00044-016-1677-9

Cited by 35 publications

(24 citation statements)

References 24 publications

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“…This was briefly done by the addition of hydrogen atoms to the enzymes and cleansing of all undesirable interactions. Consequently, the binding site was defined through the determination of the binding manner of the bioactive conformation for the lead compounds cocrystallized with HPA, MGAM and AR Then, the identified compounds were docked within the active site in which CHARMm force field was allocated and the binding energies for the best docking poses were computed as described before …”

Section: Methodsmentioning

confidence: 99%

Antihyperglycaemic activity of the methanol extract from leaves of Eremophila maculata (Scrophulariaceae) in streptozotocin-induced diabetic rats

Youssef

Ashour

Ebada

et al. 2017

Journal of Pharmacy and Pharmacology

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Section: Methodsmentioning

confidence: 99%

Antihyperglycaemic activity of the methanol extract from leaves of Eremophila maculata (Scrophulariaceae) in streptozotocin-induced diabetic rats

Youssef

Ashour

Ebada

et al. 2017

Journal of Pharmacy and Pharmacology

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“…In vitro antioxidant activities of the bark extract were determined using two commonly assays DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and FRAP [ 43 ] following the protocol described by [ 44 ]. In brief, DPPH scavenging capacity assay was done following the standard assay reported by Blois [ 45 ] with some modifications adapted to a 96-well microplate.…”

Section: Methodsmentioning

confidence: 99%

Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract

et al. 2017

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Natural products are considered as an important source for the discovery of new drugs to treat aging-related degenerative diseases and liver injury. The present study profiled the chemical constituents of a methanol extract from Senna singueana bark using HPLC-PDA-ESI-MS/MS and 36 secondary metabolites were identified. Proanthocyanidins dominated the extract. Monomers, dimers, trimers of (epi)catechin, (epi)gallocatechin, (epi)guibourtinidol, (ent)cassiaflavan, and (epi)afzelechin represented the major constituents. The extract demonstrated notable antioxidant activities in vitro: In DPPH (EC50 of 20.8 µg/mL), FRAP (18.16 mM FeSO4/mg extract) assays, and total phenolic content amounted 474 mg gallic acid equivalent (GAE)/g extract determined with the Folin-Ciocalteu method. Also, in an in vivo model, the extract increased the survival rate of Caenorhabditis elegans worms pretreated with the pro-oxidant juglone from 43 to 64%, decreased intracellular ROS inside the wild-type nematodes by 47.90%, and induced nuclear translocation of the transcription factor DAF-16 in the transgenic strain TJ356. Additionally, the extract showed a remarkable hepatoprotective activity against d-galactosamine (d-GalN) induced hepatic injury in rats. It significantly reduced elevated AST (aspartate aminotransferase), and total bilirubin. Moreover, the extract induced a strong cytoplasmic Bcl-2 expression indicating suppression of apoptosis. In conclusion, the bark extract of S. sengueana represents an interesting candidate for further research in antioxidants and liver protection.

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“…Then the cells were maintained as a monolayer culture adopting serial subculturing. Cells growing in the logarithmic phase were employed in all experiments [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Study of the anti-allergic and anti-inflammatory activity of Brachychiton rupestris and Brachychiton discolor leaves (Malvaceae) using in vitro models

Thabet

Youssef

Korinek

et al. 2018

BMC Complement Altern Med

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BackgroundBrachychiton rupestris and Brachychiton discolor (Malvaceae) are ornamental trees native to Australia. Some members of Brachychiton and its highly related genus, Sterculia, are employed in traditional medicine for itching, dermatitis and other skin diseases. However, scientific studies on these two genera are scarce. Aiming to reveal the scientific basis of the folk medicinal use of these plants, the cytotoxicity, anti-inflammatory and anti-allergic activities of Brachychiton rupestris and Brachychiton discolor leaves extracts and fractions were evaluated. Also, phytochemical investigation of B. rupestris was performed to identify the compounds exerting the biological effect.MethodsExtracts as well as fractions of Brachychiton rupestris and Brachychiton discolor were tested for their cytotoxicity versus hepatoma HepG2, lung A549, and breast MDA-MB-231 cancer cell lines. Assessment of the anti-allergic activity was done using degranulation assay in RBL-2H3 mast cells. Anti-inflammatory effect was tested by measuring the suppression of superoxide anion production as well as elastase release in fMLF/CB-induced human neutrophils. Phytochemical investigation of the n-hexane, dichloromethane and ethyl acetate fractions of B. rupestris was done using different chromatographic and spectroscopic techniques.ResultsThe tested samples showed no cytotoxicity towards the tested cell lines. The nonpolar fractions of both B. rupestris and B. discolor showed potent anti-allergic potency by inhibiting the release of β-hexosaminidase. The dichloromethane fraction of both species exhibited the highest anti-inflammatory activity by suppressing superoxide anion generation and elastase release with IC50 values of 2.99 and 1.98 μg/mL, respectively for B. rupestris, and 0.78 and 1.57 μg/mL, respectively for B. discolor. Phytochemical investigation of various fractions of B. rupestris resulted in the isolation of β-amyrin acetate (1), β-sitosterol (2) and stigmasterol (3) from the n-hexane fraction. Scopoletin (4) and β-sitosterol-3-O-β-D-glucoside (5) were obtained from the dichloromethane fraction. Dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucoside (6) and dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucoside (7) were separated from the ethyl acetate fraction. Scopoletin (4) showed anti-allergic and anti-inflammatory activity.ConclusionsIt was concluded that the nonpolar fractions of both Brachychiton species exhibited anti-allergic and anti-inflammatory activities.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2359-6) contains supplementary material, which is available to authorized users.

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Chemical profiling of Phlomis thapsoides (Lamiaceae) and in vitro testing of its biological activities

Cited by 35 publications

References 24 publications

Antihyperglycaemic activity of the methanol extract from leaves of Eremophila maculata (Scrophulariaceae) in streptozotocin-induced diabetic rats

Antihyperglycaemic activity of the methanol extract from leaves of Eremophila maculata (Scrophulariaceae) in streptozotocin-induced diabetic rats

Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract

Study of the anti-allergic and anti-inflammatory activity of Brachychiton rupestris and Brachychiton discolor leaves (Malvaceae) using in vitro models

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