2009
DOI: 10.1016/j.foodchem.2008.10.081
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Chemical properties and antioxidant activity of exopolysaccharides from mycelial culture of Cordyceps sinensis fungus Cs-HK1

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Cited by 194 publications
(114 citation statements)
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“…12) One g of tobacco flower powder (TFP) was extracted with distilled water in a JY92-2D Ultrasonic cell grinder (Ningbo Scientz Biotechnology Co., Ltd., Ningbo, China). Extraction was carried out at a variety of ultrasound power levels (200-400 W) at various temperatures (50-70 C), ratios of solvent to solid (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35); v/w), and extraction times (2-6 min). Debris fragments of the polysaccharide extracts were removed by centrifugation.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…12) One g of tobacco flower powder (TFP) was extracted with distilled water in a JY92-2D Ultrasonic cell grinder (Ningbo Scientz Biotechnology Co., Ltd., Ningbo, China). Extraction was carried out at a variety of ultrasound power levels (200-400 W) at various temperatures (50-70 C), ratios of solvent to solid (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35); v/w), and extraction times (2-6 min). Debris fragments of the polysaccharide extracts were removed by centrifugation.…”
Section: Methodsmentioning
confidence: 99%
“…The antioxidant activity of polysaccharides, might be attributed to their hydroxyl groups and other functional groups, such as -COOH, C=O, and -O-, which can donate electrons, reducing the radicals to a more stable form or reacting with free radicals to terminate the radical chain reaction. 25) In conclusion, the orthogonal matrix method was applied to optimize ultrasound-assisted extraction parameters for polysaccharides, from flue-cured tobacco flowers. The highest yield of crude polysaccharide, 3.76% (w/w), was obtained under optimal conditions: ultrasound power 300 W, extraction time 4 min, ratio of water to raw material of 35:1 (mL/g), and extraction temperature 70 C. Two fractions of polysaccharides were obtained through purification of crude polysaccharide by Sepharose CL-6B column chromatography.…”
Section: Antioxidant Property Analysis Of the Polysaccharidesmentioning
confidence: 99%
“…The antioxidant activity of polysaccharides might be attributed to their hydroxyl groups and other functional groups, such as C=O, -COOH and -O-, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction (Leung et al 2009). The superior antioxidant activities of EPS fraction with sucrose as carbon source as compared to the other two EPS fractions may be attributed to the differing molecular properties, i.e.…”
Section: Antioxidant Properties Analysismentioning
confidence: 99%
“…2, the largest absorption band at 3380 cm −1 is assigned to the hydroxyl group and the small band at 2921 cm −1 are due to C-H-stretching vibration. The absorption bands at 1640 and 1542 cm −1 are attributed to the stretching vibration of the carbonyl bond, C-O of the amide group and the bending vibration of the N-H bond in peptides or proteins (Leung et al, 2009). The stretching peak at 1089 and 1026 cm −1 are suggestive of a C-O bond (Yan et al, 2010).…”
Section: Ft-ir Spectrum Of Cpsmentioning
confidence: 99%