“…The antioxidant activity of polysaccharides, might be attributed to their hydroxyl groups and other functional groups, such as -COOH, C=O, and -O-, which can donate electrons, reducing the radicals to a more stable form or reacting with free radicals to terminate the radical chain reaction. 25) In conclusion, the orthogonal matrix method was applied to optimize ultrasound-assisted extraction parameters for polysaccharides, from flue-cured tobacco flowers. The highest yield of crude polysaccharide, 3.76% (w/w), was obtained under optimal conditions: ultrasound power 300 W, extraction time 4 min, ratio of water to raw material of 35:1 (mL/g), and extraction temperature 70 C. Two fractions of polysaccharides were obtained through purification of crude polysaccharide by Sepharose CL-6B column chromatography.…”