Chemical reagents modulate nucleic acid-activated toll-like receptors

Xiao, Li; Sun, Xinyuan; Guo, Xiaohong; Li, Xueren; Peng, Shouchun; Mu, Xin

doi:10.1016/j.biopha.2022.112622

Cited by 9 publications

(6 citation statements)

References 125 publications

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“…At least 13 TLRs have been identified in mammals, and previous studies have localized TLR3, TLR7, TLR8, and TLR9 to the endosomes [ 24 , 25 , 26 ]. TLR3 specifically recognizes dsRNA, while TLR7 and TLR8 recognize ssRNA [ 24 , 25 ]. TLR9 recognizes DNA, particularly hypomethylated CpG-containing DNA [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of interleukin-10 induction in murine spleen and RAW264 cells by Latilactobacillus curvatus K4G4 isolated from fermented Brassica rapa L.

OKANO,

TANAKA,

YAMADA

et al. 2024

Bioscience of Microbiota, Food and Health

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Lactic acid bacteria (LAB) are commonly used in fermented foods, and some LAB modulate the immune response. We aimed to investigate the mechanism by which LAB isolates from fermented Brassica rapa L. induce the production of anti-inflammatory interleukin (IL)-10 by the murine spleen and RAW264 cells. Spleen cells from BALB/c mice or the mouse macrophage cell line RAW264 were cultured with heat-killed LAB isolated from fermented B. rapa L., and the IL-10 level in the supernatant was measured. Latilactobacillus curvatus K4G4 provided the most potent IL-10 induction among 13 isolates. Cell wall components of K4G4 failed to induce IL-10, while treatment of the bacteria with RNase A under a high salt concentration altered K4G4 induction of IL-10 by spleen cells. In general, a low salt concentration diminished the IL-10 induction by all strains, including K4G4. In addition, chloroquine pretreatment and knock down of toll-like receptor 7 through small interfering RNA suppressed K4G4 induction of IL-10 production by RAW264 cells. Our results suggest that single-stranded RNA from K4G4 is involved, via endosomal toll-like receptor 7, in the induction of IL-10 production by macrophages. K4G4 is a promising candidate probiotic strain that modulates the immune response by inducing IL-10 from macrophages.

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Section: Discussionmentioning

confidence: 99%

Mechanisms of interleukin-10 induction in murine spleen and RAW264 cells by Latilactobacillus curvatus K4G4 isolated from fermented Brassica rapa L.

OKANO,

TANAKA,

YAMADA

et al. 2024

Bioscience of Microbiota, Food and Health

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“…The greater the sensitivity of melanoma cells to IFN-γ, the lower the expression of SOCS3, and the lowering of SOCS3 expression in melanoma cells by IFN-γ may significantly stimulate the production of STAT1 ( 56 ). TLR7 (toll-like receptors 7) is an endosomal pattern recognition receptor, when activated, causes type I interferons and inflammatory reactions ( 57 ). TLR7 plays a crucial role in activating both natural and acquired immune responses and has an activating effect on virtually all cells engaged in the tumor immune response ( 58 ).…”

Section: Discussionmentioning

confidence: 99%

Construction of immunotherapy-related prognostic gene signature and small molecule drug prediction for cutaneous melanoma

Xing

Jia

et al. 2022

Front. Oncol.

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BackgroundCutaneous melanoma (CM), a kind of skin cancer with a high rate of advanced mortality, exhibits a wide variety of driver and transmitter gene alterations in the immunological tumor microenvironment (TME) associated with tumor cell survival and proliferation.MethodsWe analyzed the immunological infiltration of TME cells in normal and malignant tissues using 469 CM and 556 normal skin samples. We used a single sample gene set enrichment assay (ssGSEA) to quantify the relative abundance of 28 cells, then used the LASSO COX regression model to develop a riskScore prognostic model, followed by a small molecule drug screening and molecular docking validation, which was then validated using qRT-PCR and IHC.ResultsWe developed a prognosis model around seven essential protective genes for the first time, dramatically elevated in tumor tissues, as did immune cell infiltration. Multivariate Cox regression results indicated that riskScore is an independent and robust prognostic indicator, and its predictive value in immunotherapy was verified. Additionally, we identified Gabapentin as a possible small molecule therapeutic for CM.ConclusionsA riskScore model was developed in this work to analyze patient prognosis, TME cell infiltration features, and treatment responsiveness. The development of this model not only aids in predicting patient response to immunotherapy but also has significant implications for the development of novel immunotherapeutic agents and the promotion of tailored treatment regimens.

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“…The greater the sensitivity of melanoma cells to IFN-γ, the lower the expression of SOCS3, and the lowering of SOCS3 expression in melanoma cells by IFN-γ may signi cantly stimulate the production of STAT1 [48]. TLR7 (toll-like receptors 7) is an endosomal pattern recognition receptor, when activated, causes type I interferons and in ammatory reactions [49]. TLR7 plays a crucial role in activating both natural and acquired immune responses and has an activating effect on virtually all cells engaged in the tumor immune response [50].…”

Section: Discussionmentioning

confidence: 99%

Construction of immunotherapy-related prognostic gene signature and small molecule drug prediction for cutaneous melanoma

Xing

Jia

Han

2022

Preprint

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Background Cutaneous melanoma (CM), a kind of skin cancer with a high rate of advanced mortality, exhibits a wide variety of driver and transmitter gene alterations in the immunological tumor microenvironment (TME) associated with tumor cell survival and proliferation. Methods We analyzed the immunological infiltration of TME cells in normal and malignant tissues using 469 CM and 556 normal skin samples. We used a single sample gene set enrichment assay (ssGSEA) to quantify the relative abundance of 28 cells, developed a riskScore prognostic model using the LASSO COX regression model, and performed small molecule drug screening and molecular docking validation, which was finally validated using qRT-PCR and IHC. Results We developed a prognosis model around seven essential protective genes for the first time, dramatically elevated in tumor tissues, as did immune cell infiltration. The results of multivariate Cox regression demonstrated that riskScore is an independent and robust prognostic indicator, and its predictive usefulness in immunotherapy was verified. Additionally, we identified Gabapentin as a possible small molecule therapeutic for CM. Conclusions A riskScore model was developed in this work to analyze patient prognosis, TME cell infiltration features, and treatment responsiveness. The development of this model aid in predicting patient response to immunotherapy, but it also has significant implications for the development of novel immunotherapeutic agents and the promotion of tailored treatment regimens.

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Chemical reagents modulate nucleic acid-activated toll-like receptors

Cited by 9 publications

References 125 publications

Mechanisms of interleukin-10 induction in murine spleen and RAW264 cells by <i>Latilactobacillus curvatus</i> K4G4 isolated from fermented <i>Brassica rapa</i> L.

Mechanisms of interleukin-10 induction in murine spleen and RAW264 cells by <i>Latilactobacillus curvatus</i> K4G4 isolated from fermented <i>Brassica rapa</i> L.

Construction of immunotherapy-related prognostic gene signature and small molecule drug prediction for cutaneous melanoma

Construction of immunotherapy-related prognostic gene signature and small molecule drug prediction for cutaneous melanoma

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