Chemical Signals of Vector Beetle Facilitate the Prevalence of a Native Fungus and the Invasive Pinewood Nematode

Zhang, Bin; Zhang, Wei; Lü, Min; Ahmad, Faheem; Tian, Huanfang; Ning, Jing; Liu, Xiaolong; Zhao, Lilin; Jian, Sun

doi:10.21307/jofnem-2017-081

Cited by 16 publications

(4 citation statements)

References 70 publications

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“…Our work and that of others have shown that the combined temperature and certain metal exposure could induce toxicity and stress protein response in human endothelial cells, Caenorhabditis elegans , Mytilus galloprovincialis , Larimichthys crocea , Triticum aestivum L . It has also been proposed that the heterologous overexpression of both prokaryotic and eukaryotic sHSPs could enhance organism tolerance and adaptation to stress conditions .…”

Section: Introductionsupporting

confidence: 56%

“…[18,19] In vitro studies have demonstrated that AgsA formed fibrils and possesses strong chaperone-like activity at elevated temperature. [20] Our work and that of others have shown that the combined temperature and certain metal exposure could induce toxicity and stress protein response in human endothelial cells, [21] Caenorhabditis elegans, [22,23] Mytilus galloprovincialis, [24] Larimichthys crocea, [25] Triticum aestivum L. [26] It has also been proposed that the heterologous overexpression of both prokaryotic and eukaryotic sHSPs could enhance organism tolerance and adaptation to stress conditions. [19,[27][28][29][30] However, literature describing the combined effects of heavy metals and temperatures, and the heat shock proteins response mainly focused on aquatic organisms [8,31] and still are relatively scarce on E. coli cells.…”

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confidence: 99%

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AgsA response to cadmium and copper effects at different temperatures in Escherichia coli

Ezemaduka

Wang

et al. 2019

J Biochem & Molecular Tox

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Small heat shock proteins (sHsps), present from prokaryotes to eukaryotes, are a highly conserved molecular chaperone family. They play a crucial role in protecting organisms against cellular insults from single or multiple environmental stressors including heavy metal exposure, heat or cold shock, oxidative stress, desiccation, etc. Here, the toxicity of cadmium and copper, and their ability to modify the cellular growth rate at different temperatures in Escherichia coli cells were tested. Also, the response mechanism of the sHSP aggregation‐suppressing protein (AgsA) in such multiple stress conditions was investigated. The results showed that the half effect concentration (EC50) of cadmium in AgsA‐transformed E. coli cells at 37°C, 42°C, and 50°C were 11.106, 29.50, and 4.35 mg/L, respectively, and that of the control cells lacking AgsA were 5.05, 0.93, and 0.18 mg/L, respectively, while the half effect concentration (EC50) of copper in AgsA‐transformed E. coli cells at 37°C, 42°C, and 50°C were 27.3, 3.40, and 1.28 mg/L, respectively, and that of the control cells lacking AgsA were 27.7, 5.93, and 0.134 mg/L, respectively. The toxicities of cadmium and copper at different temperatures as observed by their modification of the cellular growth rate and inhibitory effects were in a dose‐dependent manner. Additionally, biochemical characterization of AgsA protein in cells subjected to cadmium and copper stresses at different temperatures implicated suppressed aggregation of cellular proteins in AgsA‐transformed E. coli cells. Altogether, our data implicate the AgsA protein as a sensitive protein‐based biomarker for metal‐induced toxicity monitoring.

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Section: Introductionsupporting

confidence: 56%

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confidence: 99%

AgsA response to cadmium and copper effects at different temperatures in Escherichia coli

Ezemaduka

Wang

et al. 2019

J Biochem & Molecular Tox

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“…Heat shock proteins are stress-responsive proteins and play an important role in protecting cells from elevated environments. CeHSP17 was significantly up-regulated by cadmium and zinc in wild-type C. elegans [36]. In transgenic Arabidopsis, PfHSP17.2 showed a higher resistant ability under heat, cold and salt stresses [37].…”

Section: Stress Induced Oxidative Injuries and Inhibited Photosyntmentioning

confidence: 96%

Changes in Proteome and Protein Phosphorylation Reveal the Protective Roles of Exogenous Nitrogen in Alleviating Cadmium Toxicity in Poplar Plants

Huang

Tian

et al. 2019

IJMS

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Phytoremediation soil polluted by cadmium has drawn worldwide attention. However, how to improve the efficiency of plant remediation of cadmium contaminated soil remains unknown. Previous studies showed that nitrogen (N) significantly enhances cadmium uptake and accumulation in poplar plants. In order to explore the important role of nitrogen in plants’ responses to cadmium stress, this study investigates the poplar proteome and phosphoproteome difference between Cd stress and Cd + N treatment. In total, 6573 proteins were identified, and 5838 of them were quantified. With a fold-change threshold of > 1.3, and a p-value < 0.05, 375 and 108 proteins were up- and down-regulated by Cd stress when compared to the control, respectively. Compared to the Cd stress group, 42 and 89 proteins were up- and down-regulated by Cd + N treatment, respectively. Moreover, 522 and 127 proteins were up- and down-regulated by Cd + N treatment compared to the CK group. In addition, 1471 phosphosites in 721 proteins were identified. Based on a fold-change threshold of > 1.2, and a p-value < 0.05, the Cd stress up-regulated eight proteins containing eight phosphosites, and down-regulated 58 proteins containing 69 phosphosites, whereas N + Cd treatment up-regulated 86 proteins containing 95 phosphosites, and down-regulated 17 proteins containing 17 phosphosites, when compared to Cd stress alone. N + Cd treatment up-regulated 60 proteins containing 74 phosphosites and down-regulated 37 proteins containing 42 phosphosites, when compared to the control. Several putative responses to stress proteins, as well as transcriptional and translational regulation factors, were up-regulated by the addition of exogenous nitrogen following Cd stress. Especially, heat shock protein 70 (HSP70), 14-3-3 protein, peroxidase (POD), zinc finger protein (ZFP), ABC transporter protein, eukaryotic translation initiation factor (elF) and splicing factor 3 B subunit 1-like (SF3BI) were up-regulated by Cd + N treatment at both the proteome and the phosphoproteome levels. Combing the proteomic data and phosphoproteomics data, the mechanism by which exogenous nitrogen can alleviate cadmium toxicity in poplar plants was explained at the molecular level. The results of this study will establish the solid molecular foundation of the phytoremediation method to improve cadmium-contaminated soil.

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“…The newly eclosed beetle should have a maturation feeding in healthy trees. The nematode then departs from the spiracles driven by CO 2 enhanced by feeding behavior and invades new healthy trees via the feeding wounds 14 , 15 , thus starting a new cycle of infection, propagation and dispersal. While the chemical signals among the symbiosis have been well characterized, the molecular mechanism of the chemical communications remains elusive.…”

Section: Background and Summarymentioning

confidence: 99%

A near-complete genome assembly of Monochamus alternatus a major vector beetle of pinewood nematode

Xing,

Liu,

et al. 2024

Sci Data

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The Japanese sawyer beetle, Monochamus alternatus, is not only one of the most important wood boring pest itself, but also a major vector of the invasive pinewood nematode (PWN), which is the causal agent of the devastative pine wilt disease (PWD) and threats the global pine forest. Here, we present a near-complete genome of M. alternatus at the chromosome level. The assembled genome was 792.05 Mb with contig N50 length of 55.99 Mb, which is the largest N50 size among the sequenced Coleoptera insects currently. 99.57% of sequence was anchored onto ten pseudochromosomes (one X-chromosome and nine autosomes), and the final genome harbored only 13 gaps. BUSCO evaluation revealed the presence of 99.0% of complete core genes. Thus, our genome assembly represented the highest-contiguity genome assembly as well as high completeness in insects so far. We identified 20,471 protein-coding genes, of which 20,070 (98.04%) were functionally annotated. The genome assembly of M. alternatus provides a valuable resource for exploring the evolution of the symbiosis between PWN and the vector insects.

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Chemical Signals of Vector Beetle Facilitate the Prevalence of a Native Fungus and the Invasive Pinewood Nematode

Cited by 16 publications

References 70 publications

AgsA response to cadmium and copper effects at different temperatures in Escherichia coli

AgsA response to cadmium and copper effects at different temperatures in Escherichia coli

Changes in Proteome and Protein Phosphorylation Reveal the Protective Roles of Exogenous Nitrogen in Alleviating Cadmium Toxicity in Poplar Plants

A near-complete genome assembly of Monochamus alternatus a major vector beetle of pinewood nematode

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