Chemical stress-responsive genes from the lignin-degrading fungus Phanerochaete chrysosporium exposed to dibenzo-p-dioxin

Abstract

The stress-responsive genes expressed against the exogenous addition of dibenzo-p-dioxin from the lignin-degrading basidiomycete, Phanerochaete chrysosporium, were determined utilizing a differential display reverse transcription-PCR technique. Six cDNA fragments, exhibiting a high homology with various proteins from other microorganisms, were identified via a BLAST search; that is, NADHubiquinone oxidoreductase (NUO), ATP/ADP carrier, uric acid-xanthine permease, manganese superoxide dismutase, 3-hydroxybutyrylcoenzyme A dehydrogenase, and cytoskeletal protein. The expression of NUO was also up-regulated by catechol and trihydroxybenzene but not by dibenzofuran, suggesting that NUO expression was initiated by the formation of quinone products through the reaction of extracellular one-electron oxidizing enzymes with dibenzo-p-dioxin. ß

Role of P450 Monooxygenases in the Degradation of the Endocrine-Disrupting Chemical Nonylphenol by the White Rot Fungus Phanerochaete chrysosporium

“…5B). This finding is in contrast to the observed upregulation of uap in Aspergillus nidulans and P. chrysosporium in response to other forms of chemical stress (13,27). This result may be a consequence of the general FIG.…”

Adaptations in microbiological populations exposed to dinitrophenol and other chemical stressors

“…Microbiological stress responses have been observed on exposure to many environmentally relevant chemicals, including phenols [1], chlorophenols [2,3], dinitrophenols [4,5], toluene [6], methyltert-butyl ether [7], explosive 2,4,6-Trinitrotoluene [8], explosive cyclotrimethylenetrinitramine [9], and dibenzo-p-dioxin [10]. Microorganisms that are effective at degrading pollutants can be stressed by exposure to these chemicals, as has been observed for Methylocystis sp.…”

Changes in the Gene Expression of the White Rot FungusPhanerochaete chrysosporiumDue to the Addition of Atropine

“…29) There are many enzymes which were possibly related to biodegradation of xenobiotics in Table 2 Genes probably related to stress response system Chemical stress or oxidative stress induces ligninolytic enzyme expression. [30][31][32][33] In this experiment, P. chrysosporium culture was exposed to pure oxygen gas which is required to cause this aerobic fungus to express ligninolytic enzymes, so many reactive oxygen species should arise in the culture. Glutaredoxin, glutathione transferase, and glutathione S-transferase, described above, are also known as stress response genes.…”