Chemical surface modifications for the development of silicon-based label-free integrated optical (IO) biosensors: A review

Bañuls, María‐José; Puchades, Rosa; Maquieira, Ángel

doi:10.1016/j.aca.2013.01.025

Cited by 124 publications

(107 citation statements)

References 165 publications

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“…Also polymerization frequently occurs during their assembly. 1 The characteristics of a silane based coating depend on many factors such as density, 2 chain length and functional group. 3 Having a coating with simultaneously more than one function thus represents a challenge for silane based molecules, since an alteration in one directly affects the nature of whole coating.…”

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confidence: 99%

Phosphonate based organosilane modification of a simultaneously protein resistant and bioconjugable silica surface

2014

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A facile method to coat silica surfaces with THPMP is introduced, forming simultaneously a protein resistant and bioconjugable surface.The coating is experimentally identified and its anti-fouling and bioconjugable characteristics are demonstrated.Chemical modication of surfaces, which enables unprecedented manipulation of the characteristics of materials, can be attained by a virtually indenite number of different compounds and methods. Regarding the accommodation of molecules over surfaces, self-assembly strategies depending on spontaneous interactions of the molecules with the surface for the formation of mono-or multilayers are among the most frequently utilized. Self-assembly generally occurs via functional head groups having high affinity towards the substrate, anchoring the molecule covalently or non-covalently to the surface. Among various different head groups, silanes are particularly important and widely used, while the knowledge regarding their assembly is far more empirical, besides being practical. Silanes are assumed to form monolayers over the surfaces; yet this is a controversial issue since their assembly depends highly on the environmental conditions, obstructing the formation of lms with well-dened characteristics. Also polymerization frequently occurs during their assembly. 1The characteristics of a silane based coating depend on many factors such as density, 2 chain length and functional group.3 Having a coating with simultaneously more than one function thus represents a challenge for silane based molecules, since an alteration in one directly affects the nature of whole coating. Coating with more than one molecule is hypothetically possible, either simultaneously or sequentially. However, it is not practical to perform in every case due to easy polymerization and unpredictable assembly behavior of silanes. 1Here we propose an alternative strategy for realizing antifouling surfaces with bioconjugation capability by using only a single molecule for surface modication. We utilized THPMP, a commercial small silane-based molecule, to produce a bioconjugable and anti-fouling silica surface (see ESI † for details). Phosphonates have been used as functional groups stabilizing silica containing nanoparticles against aggregation 4 or sites for covalently anchoring primary amines to other molecules such as nucleic acids.5 Also, it has been shown previously that methylphosphonate containing silica nanoparticles exhibited signicantly reduced non-specic biological interactions, 6 and our research group has recently demonstrated that methylphosphonate conjugation particularly decreased protein adsorption on silica nanoparticles.7 However, to the best of our knowledge, neither the characteristics of these phosphonatebased lms were investigated in detail, nor were their bioconjugation properties utilized together with their protein resistant characteristics. In this study, we experimentally characterized the THPMP lm, and examined it regarding its protein resistance and bioconjugation characteris...

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confidence: 99%

Phosphonate based organosilane modification of a simultaneously protein resistant and bioconjugable silica surface

2014

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“…DNA-based methods have also been employed in many 5 areas, examples being forensic science [1], environmental control [2] and biosensing [3]. 6 Additionally, new issues addressed to both array-like platforms [4] and lab-on-a-chip 7 arrangements [5] are currently under development. 8 Nearly all nucleic acid-based methods employ the recognition element immobilized on 9 a surface, so DNA immobilization has been extensively studied in the last years, and many 10 attachment chemistries have been described [6].…”

Section: Directmentioning

confidence: 99%

“…Aminated 12 oligonucleotide probes were immobilized on the chip surface by chemical silanization and 13 further covalent attachment. The chemistries employed were the classical 3-14 aminopropyltriethoxysilane/glutaraldehyde and, for comparison purposes, the novel [3][4][5][6][7][8][9][10][11][12][13][14][15] isocyanatepropyltriethoxysilane, that allows the direct attachment of the aminated probe. 16 Alternatively, a thiolated oligonucleotide was also photochemically immobilized by means of 17 a thioether linkage.…”

Section: Directmentioning

confidence: 99%

Direct and label-free monitoring oligonucleotide immobilization, non-specific binding and DNA biorecognition

López-Paz

Martinez

Escorihuela

et al. 2014

Sensors and Actuators B: Chemical

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“…This type of devices will reach its full sensing potential for (bio)sensing applications only with highly optimized surface chemistry [3,4]. For silicon-based optical biosensing, simple physisorption of proteins to the sensor surface has been reported, but for advanced applications surface modification is required.…”

Section: Introductionmentioning

confidence: 99%

Protein detection on biotin-derivatized polyallylamine by optical microring resonators

Ullien¹,

Harmsma²,

Abdulla³

et al. 2014

Opt. Express

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Silicon optical microring resonators (MRRs) are sensitive devices that can be used for biosensing. We present a novel biosensing platform based on the application of polyelectrolyte (PE) layers on such MRRs. The top PE layer was covalently labeled with biotin to ensure binding sites for antibodies via a streptavidin-biotin binding scheme. Monitoring the shift in the microring resonance wavelength allows realtime, highly sensitive detection of the biomolecular interaction.

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Chemical surface modifications for the development of silicon-based label-free integrated optical (IO) biosensors: A review

Cited by 124 publications

References 165 publications

Phosphonate based organosilane modification of a simultaneously protein resistant and bioconjugable silica surface

Phosphonate based organosilane modification of a simultaneously protein resistant and bioconjugable silica surface

Direct and label-free monitoring oligonucleotide immobilization, non-specific binding and DNA biorecognition

Protein detection on biotin-derivatized polyallylamine by optical microring resonators

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