Chemically Functionalized Water-Soluble Single-Walled Carbon Nanotubes Obstruct Vesicular/Plasmalemmal Recycling in Astrocytes Down-Stream of Calcium Ions

Gottipati, Manoj K.; Bekyarova, Elena; Haddon, Robert C.; Parpura, Vladimir

doi:10.3390/cells9071597

Cited by 2 publications

(2 citation statements)

References 59 publications

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“…While out of scope of the present study, of additional interest would be to compare the cytokine release pattern from astrocytes exposed to SWCNT-PEG to that of astrocytes exposed to known astrocytic secretagogues, such as adenosine triphosphate. There is a three-pronged rationale for this endeavor: (i) this nucleotide is increased in the extracellular space in experimental TBI [73]; (ii) it can cause glutamate release from cultured uninjured astrocytes via regulated exocytosis, i.e., vesicular release [74,75]; and SCWNT-PEGs affect vesicular recycling in cultured uninjured astrocytes [76].…”

Section: Swcnt-peg Modulate the Secretory Function Of Astrocytes Expo...mentioning

confidence: 99%

Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro

Gržeta Krpan,

Harej Hrkać,

Janković

et al. 2024

Cells

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We tested the effects of water-soluble single-walled carbon nanotubes, chemically functionalized with polyethylene glycol (SWCNT-PEG), on primary mouse astrocytes exposed to a severe in vitro simulated traumatic brain injury (TBI). The application of SWCNT-PEG in the culture media of injured astrocytes did not affect cell damage levels, when compared to those obtained from injured, functionalization agent (PEG)-treated cells. Furthermore, SWCNT-PEG did not change the levels of oxidatively damaged proteins in astrocytes. However, this nanomaterial prevented the reduction in plasmalemmal glutamate transporter EAAT1 expression caused by the injury, rendering the level of EAAT1 on par with that of control, uninjured PEG-treated astrocytes; in parallel, there was no significant change in the levels of GFAP. Additionally, SWCNT-PEG increased the release of selected cytokines that are generally considered to be involved in recovery processes following injuries. As a loss of EAATs has been implicated as a culprit in the suffering of human patients from TBI, the application of SWCNT-PEG could have valuable effects at the injury site, by preventing the loss of astrocytic EAAT1 and consequently allowing for a much-needed uptake of glutamate from the extracellular space, the accumulation of which leads to unwanted excitotoxicity. Additional potential therapeutic benefits could be reaped from the fact that SWCNT-PEG stimulated the release of selected cytokines from injured astrocytes, which would promote recovery after injury and thus counteract the excess of proinflammatory cytokines present in TBI.

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Section: Swcnt-peg Modulate the Secretory Function Of Astrocytes Expo...mentioning

confidence: 99%

Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro

Gržeta Krpan,

Harej Hrkać,

Janković

et al. 2024

Cells

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“…89 Another study shows that SW-CNT-PEG solute causes a reduction in the ATP-induced glutamate release but does not affect the intracellular Ca2+ elevations in astrocytes. 94 In a study, self-standing 3D meshes of interconnected MWCNTs were manufactured. Such three-dimensional (3D) mesh of pure carbon nanotubes can open new horizons in treating injured CNS tissues, such as the spinal cord.…”

Section: Cnts In Tsci Modelsmentioning

confidence: 99%

Nanoparticles in traumatic spinal cord injury: therapy and diagnosis

Mousa¹,

Mohammad²,

Rezk³

et al. 2021

F1000Res

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Nanotechnology has been previously employed for constructing drug delivery vehicles, biosensors, solar cells, lubricants and as antimicrobial agents. The advancement in synthesis procedure makes it possible to formulate nanoparticles (NPs) with precise control over physico-chemical and optical properties that are desired for specific clinical or biological applications. The surface modification technology has further added impetus to the specific applications of NPs by providing them with desirable characteristics. Hence, nanotechnology is of paramount importance in numerous biomedical and industrial applications due to their biocompatibility and stability even in harsh environments. Traumatic spinal cord injuries (TSCIs) are one of the major traumatic injuries that are commonly associated with severe consequences to the patient that may reach to the point of paralysis. Several processes occurring at a biochemical level which exacerbate the injury may be targeted using nanotechnology. This review discusses possible nanotechnology-based approaches for the diagnosis and therapy of TSCI, which have a bright future in clinical practice.

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Chemically Functionalized Water-Soluble Single-Walled Carbon Nanotubes Obstruct Vesicular/Plasmalemmal Recycling in Astrocytes Down-Stream of Calcium Ions

Cited by 2 publications

References 59 publications

Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro

Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro

Nanoparticles in traumatic spinal cord injury: therapy and diagnosis

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