“…A few bioanalytical methods based on spectrophotometry, electrochemical analysis using modified ion‐selective electrodes, capillary electrophoresis, gas chromatography coupled with a nitrogen sensitive detector and another with mass detector, thin‐layer chromatography and HPLC‐UV have been reported in the literature for the quantification of PBD in various biological matrices (Abdel‐Gawad, Issa, Hassouna, & Hussien, ; Abdel‐Hay, Sabry, Barary, & Belal, ; Altiokka, Can, & Aboul‐Enein, ; Fanelli & Frigerio, ; Ibrahim, ; Issa, Abou‐Attia, Abdel‐Gawad, & Abdel‐Hamid, ; Issa, Hassouna, Abdel‐Gawad, & Hussien, ; Jenner, Taylor, & Campbell, ; Sarati, Guiso, Spinelli, & Caccia, ). Spectrophotometric methods lack selectivity while electroanalytical methods are not commonly used in pharmaceutical analysis.…”