Chemically modified magnetic immobilized phospholipase A1 and its application for soybean oil degumming

Effective Soybean Oil Degumming by Immobilized Phospholipases A2 from Walterinnesia aegyptia Venom

“…More recently, numerous studies have focused on enzymatic degumming because of its multiple advantages over physical and chemical techniques including better yields of refined oil, milder reaction conditions, and lesser chemical waste. , Various enzymes have been reported to be efficient for oil degumming, such as cholesterol/glycerophospholipid acyltransferases and phospholipasesA (PLA) and (PLC). Among PLA, the most commonly used are PLA 1 and PLA 2 converting nonhydratable PLs into their hydratable forms (lysoPLs), thus facilitating wash-out and definitely enhancing vegetable oil refining. ,, However, the industrial applications of freePLA 1 are limited by its thermal/organic solvents instability, pH requirements, and commercial unavailability, whereas several PLA 2 s are lately being the most often-employed biocatalysts for the PL hydrolysis …”

“…1,8,9 However, the industrial applications of freePLA 1 are limited by its thermal/organic solvents instability, pH requirements, and commercial unavailability, whereas several PLA 2 s are lately being the most often-employed biocatalysts for the PL hydrolysis. 10 Although the enzymes' industrial application is nowadays well established, there is still considerable research to be carried out on the development of novel applications as well as the improvement of current processes trough using promising technologies such as immobilization. 11 Compared to their free form, immobilized enzymes are preferred since they offer several advantageous especially higher stability, economic convenience, and ability of separation from reaction mixtures after hydrolysis and reuse of the insoluble enzyme several times, resulting in lower operating costs.…”

“… 1 , 8 , 9 However, the industrial applications of freePLA 1 are limited by its thermal/organic solvents instability, pH requirements, and commercial unavailability, whereas several PLA 2 s are lately being the most often-employed biocatalysts for the PL hydrolysis. 10 …”

Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming

“…Recently, PLA1s are widely accepted as the best candidate for enzymatic degumming 7,16,17 . Not only PLA1s are readily available, but their hydrolysis products sn ‐2‐acyl‐LPLs are unstable and can be converted to sn ‐1‐acyl‐LPLs.…”

“…Recently, PLA1s are widely accepted as the best candidate for enzymatic degumming. 7,16,17 Not only PLA1s are readily available, but their hydrolysis products sn-2-acyl-LPLs are unstable and can be converted to sn-1-acyl-LPLs. Thus, the later can be further hydrolyzed by PLA1 to release fatty acids and glycerophospholipid (GPL), which have the very strong hydrophilicity and can be easily removed by water degumming.…”