Chemiliminescence determination of lipid hydroperoxides in biological fluids

Проскурнина, Е. В.; Dzhatdoeva, A. A.; Lobichenko, E. N.; Shalina, R.I.; Vladimirov, Yu. A.

doi:10.1134/s1061934817050094

Cited by 7 publications

(6 citation statements)

References 18 publications

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“…In our study, an increase in T max was observed with spontaneous and induced chemiluminescence in patients with MJ and bilirubin levels less than 60 µmol/L. This indicator reflects the duration for the development of the maximum activity of ROS synthesis from the moment of antigenic induction of phagocytes' respiratory explosion and depends on the state of cells' metabolic reactions 12,28 . Their values, as a rule, decrease in the case of acute infectious and inflammatory diseases when neutrophils are in the activated state and increase in chronic inflammatory processes [29][30][31] .…”

Section: Discussionsupporting

confidence: 49%

“…This is manifested through phagocytosis process violations, changes in interleukin levels, neutrophil degranulation, and oxidative stress development [8][9][10][11] . Neutrophils' functional activity depends on the intensity of the respiratory burst process, and intracellu-lar metabolism 12,13 . The chemiluminescence (ChL) method allows the analysis of the features of a respiratory burst in spontaneous and induced states in various diseases 14 .…”

Section: Introductionmentioning

confidence: 99%

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Neutrophils’ functional activity in patients with mechanical jaundice and different levels of bilirubin

et al. 2021

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Introduction: Mechanical jaundice (MJ) or bile duct blockage occurs when the bile ducts' patency is impaired, and the bile flow has stopped. One of the main pathogenetic factors developing complications with MJ is immune system imbalance, particularly its phagocytic link. The purpose of the study was to understand neutrophils' functional activity dependence with different blood bilirubin levels in men with mechanical jaundice. Methods: Forty-seven middle-aged men with mechanical jaundice were divided into three groups depending on the bilirubin levels in their blood: less than 60 μmol/L (n = 10), 60 – 200 μmol/L (n = 20), and more than 200 μmol/L (n = 17). The control group consisted of 50 practically healthy men of the same age. The neutrophils' functional state was assessed using the methods of spontaneous and induced luminol-dependent chemiluminescence of neutrophils. Results: In the group of patients with mechanical jaundice and a bilirubin level of less than 60 μmol/L, there was an increase in the values of T max spontaneous by 96%, I max spontaneous by 44.81%, S spontaneous by 224.6%, T max induced by 19.9%, I max induced by 13.5%, and S induced by 140.3%. In the group with bilirubin levels from 60 – 200 μmol/L, there was an increase in the values of T max spontaneous by 86.8%, I max spontaneous at 47.7%, S spontaneous at 204.6%, I max induced at 28.3%, S induced at 445%, and activation index at 70%. The group with bilirubin levels more than 200 μmol/L showed an increase in the level of T max spontaneous by 85.9%, I max spontaneous by 53.4%, S spontaneous by 927.3%, I max induced by 28.6%, S induced by 1045%, and activation index by 92.3% compared with the control values. The intergroup differences were found in S spontaneous levels, which were higher in the group with more than 200 μmol/L bilirubin levels compared with the 60 – 200 μmol/L group and less than the 60 μmol/L groups by 216.9% and 237.3% respectively. Conclusion: The revealed changes characterize the functional activity of neutrophils' increase with an increase in the bilirubin levels in patients with mechanical jaundice.

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Section: Discussionsupporting

confidence: 49%

Section: Introductionmentioning

confidence: 99%

Neutrophils’ functional activity in patients with mechanical jaundice and different levels of bilirubin

et al. 2021

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“…The reaction in CL was based on lipid-hydroperoxide decomposition with HO − ions in a DMF/KOH/oil mixture and with acridine in addition. Finally, for the routine clinical analysis of the total amount of lipid hydroperoxide in small volumes of biological fluids, a CL method was selected using a system based on microperoxidase and isoluminol [ 56 ].…”

Section: Chemiluminescence (Cl) Spectroscopymentioning

confidence: 99%

Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives

Kontogianni

Gerothanassis

2022

Molecules

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Mono- and polyunsaturated lipids are particularly susceptible to peroxidation, which results in the formation of lipid hydroperoxides (LOOHs) as primary nonradical-reaction products. LOOHs may undergo degradation to various products that have been implicated in vital biological reactions, and thus in the pathogenesis of various diseases. The structure elucidation and qualitative and quantitative analysis of lipid hydroperoxides are therefore of great importance. The objectives of the present review are to provide a critical analysis of various methods that have been widely applied, and more specifically on volumetric methods, applications of UV-visible, infrared, Raman/surface-enhanced Raman, fluorescence and chemiluminescence spectroscopies, chromatographic methods, hyphenated MS techniques, NMR and chromatographic methods, NMR spectroscopy in mixture analysis, structural investigations based on quantum chemical calculations of NMR parameters, applications in living cells, and metabolomics. Emphasis will be given to analytical and structural methods that can contribute significantly to the molecular basis of the chemical process involved in the formation of lipid hydroperoxides without the need for the isolation of the individual components. Furthermore, future developments in the field will be discussed.

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“…There is a large number of various modifications of the chemiluminescent method [ 109 , 110 , 111 , 112 , 113 , 114 ]. The most common modification of the chemiluminescent method for determining total antioxidant capacity is based on the use of luminol (LH 2 ) as an activator of chemiluminescence.…”

Section: Methods To Evaluate Integrated Antioxidant Propertiesmentioning

confidence: 99%

Study of Antioxidant Properties of Agents from the Perspective of Their Action Mechanisms

Ivanova

Газизуллина

2020

Molecules

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The creation and analysis of a large variety of existing methods for the evaluation of integrated antioxidant properties are quite relevant in connection with a range of biological mechanisms of the antioxidants (AO) action. In this work, the existing methods are correlated with mechanisms of antioxidant action. It is shown that the results obtained by various methods are mainly incomparable. This can be connected with the implementation of various mechanisms of antioxidant action in methods. The analysis of the literature data presented in this review indicates the difficulty of creating a universal method and the feasibility of using integrated approaches based on the use of several methods that implement and combine various mechanisms of the chemical conversion of antioxidants. This review describes methods for studying the chelating ability of antioxidants, except for methods based on electron and hydrogen atom transfer reactions, which are currently not widely covered in modern literature. With the description of each mechanism, special attention is paid to electrochemical methods, as the interaction of active oxygen metabolites of radical and non-radical nature with antioxidants has an electron/proton/donor-acceptor nature, which corresponds to the nature of electrochemical methods and suggests that they can be used to study the interaction.

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Chemiliminescence determination of lipid hydroperoxides in biological fluids

Cited by 7 publications

References 18 publications

Neutrophils’ functional activity in patients with mechanical jaundice and different levels of bilirubin

Neutrophils’ functional activity in patients with mechanical jaundice and different levels of bilirubin

Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives

Study of Antioxidant Properties of Agents from the Perspective of Their Action Mechanisms

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