2007
DOI: 10.1002/bio.958
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Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol‐microperoxidase in the presence of 1‐methoxy PMS

Abstract: We developed a novel highly sensitive chemiluminescence (CL) method for BH(4). The principle of the proposed method is based on active oxygen formation induced by 1-methoxy-5-methyl phenazinium methyl sulphate (1-methoxy PMS) in the presence of dissolved oxygen. Furthermore, active oxygen is determined by a CL assay involving the luminol reaction with microperoxidase. In this report, we examined the mechanism of formation and identified the reactive oxygen species derived from BH(4) employing 1-methoxy PMS. Ad… Show more

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Cited by 3 publications
(3 citation statements)
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“…We previously developed a bioluminescence detection method for gene analysis that performs the luciferin–luciferase reaction following the transformation of pyrophosphate (PPi) produced during DNA amplification to ATP by pyruvate phosphate dikinase (PPDK) or ATP sulfurylase. [ 16 ] PPi concentrations as low as 1.56 × 10 −15 mol/assay could be detected with high sensitivity using this bioluminescence detection method. [ 17,18 ] However, the bioluminescence method using the luciferin–luciferase reaction is unsuitable for biogenic substances containing large amounts of ATP; so, a new method must be developed.…”
Section: Introductionmentioning
confidence: 99%
“…We previously developed a bioluminescence detection method for gene analysis that performs the luciferin–luciferase reaction following the transformation of pyrophosphate (PPi) produced during DNA amplification to ATP by pyruvate phosphate dikinase (PPDK) or ATP sulfurylase. [ 16 ] PPi concentrations as low as 1.56 × 10 −15 mol/assay could be detected with high sensitivity using this bioluminescence detection method. [ 17,18 ] However, the bioluminescence method using the luciferin–luciferase reaction is unsuitable for biogenic substances containing large amounts of ATP; so, a new method must be developed.…”
Section: Introductionmentioning
confidence: 99%
“…Chemiluminescence (CL) assays have been utilized for determining various kinds of compounds qualitatively and quantitatively. CL compounds have been developed as useful luminescent reagents. In particular, luminol (3-aminophthalhydrazide) derivatives, acridinium ester derivatives, and dioxetane derivatives are essential compounds in highly sensitive CL assays.…”
Section: Introductionmentioning
confidence: 99%
“…Among these, the chemiluminescence (CL) technique is rapid and has high sensitivity. This method has been applied to the measurement of biogenic substances and the sensitive detection of labeled enzymes in enzyme immunoassays (EIA) or DNA probe assays . In particular, EIA can determine specific substances in complex biological materials without isolation or purification, and has been widely applied in the field of clinical chemistry.…”
Section: Introductionmentioning
confidence: 99%