Chemiluminescence assay of alkaline phosphatase using cortisol-21 -phosphate as substrate and its application to enzyme immunoassays

Kokado, Amane; Tsuji, Akio; Maeda, Masako

doi:10.1016/s0003-2670(96)00400-x

Cited by 43 publications

(30 citation statements)

References 10 publications

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“…The CL assay of ALP using lucigenin have already been developed, using the following compounds as substrates; cortisol 21-phosphate (11), phenacyl phosphate (12), or ascorbic acid 2-phosphate (13). While dihydroxyacetone (DHA) also produces intense chemiluminescence with lucigenin, we devised CL assays of ALP using glycerol 3-phosphate or NADP as substrate and glycerol dehydrogenase (GDH) as the coupled enzyme (14).…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescent assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin

2002

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A sensitive and simple chemiluminescent assay (CL) for alkaline phosphatase (ALP) using dihydroxyacetone phosphate or its ketal (DHAP or DHAP-ketal) was developed. New substrates were transformed to dihydroxyacetone (DHA) after they were hydrolysed by ALP, which reacts with lucigenin and produces strong chemiluminescence. Under the optimum assay condition, the detection limits were 3.8 x 10(-19) and 1.5 x 10(-18) moles of ALP, respectively. The coefficients of variation (CV) at each points on the standard curve were 0.8-5.4% and 1.8-7.1% (n = 6), respectively. The mechanism of lucigenin CL with DHA was investigated by ESR spectrometry using the spin-trapping method. The mechanism was speculated as follows: the O2(-) generated by the reaction of DHA and O2 in alkaline solution reacts with lucigenin, and then emit light. The proposed CL assay was applied to the enzyme immunoassay of 17beta-oestradiol, using ALP as a label enzyme. The measurable range of 17beta-oestradiol was 15-4000-pg/mL, and the proposed method was four times more sensitive than the colorimetric assay for ALP by using 4-nitrophenyl phosphate as substrate.

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Section: Introductionmentioning

confidence: 99%

Chemiluminescent assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin

2002

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“…There are several methods to detect α-FP, such as electrochemical enzyme immunoassay [9], fluorescence immunoassay [10], enzyme-linked immunosorbent assay [11], chemiluminescence assay [12], and electrochemical assay [13]. Compared with the conventional immunoassay methods, electrochemical immunosensor offers several advantages of high Electronic supplementary material The online version of this article (doi:10.1007/s00604-015-1537-1) contains supplementary material, which is available to authorized users.…”

Section: Introductionmentioning

confidence: 99%

Sensitive electrochemical determination of α-fetoprotein using a glassy carbon electrode modified with in-situ grown gold nanoparticles, graphene oxide and MWCNTs acting as signal amplifiers

et al. 2015

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The authors describe an electrochemical immunoassay for α-fetoprotein (α-FP) using a glassy carbon electrode (GCE) modified with a nanocomposite made from gold nanoparticles, graphene oxide and multi-walled carbon nanotubes (AuNPs/GO-MWCNTs) and acting as a signal amplification matrix. The nanocomposite was synthesized in a one-pot redox reaction between GO and HAuCl 4 without using an additional reductant. The stepwise assembly of the immunoelectrode was characterized by means of cyclic voltammetry and electrochemical impedance spectroscopy. The interaction of antigen and antibody on the surface of the electrode creates a barrier for electrons and causes retarded electron transfer, this resulting in decreased signals in differential pulse voltammetry of hexacyanoferrate which is added as an electrochemical probe. Using this strategy and by working at a potential of 0.2 V (vs. SCE), a wide analytical range (0.01 -100 ng mL‾ 1 ) is covered. The correlation coefficient is 0.9929, and the limit of detection is as low as 3 pg mL‾ 1 at a signal-to-noise ratio of 3. This electrochemical immunoassay combines the specificity of an immunological detection scheme with the sensitivity of an electrode modified with AuNPs and GO-MWCNTs.

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“…A number of methods can be used to monitor CEA, such as radioimmunoassay, enzyme-linked immunosorbent assay, and chemiluminescence assay (Yuan et al, 2007;Wu et al, 2006;Kokado et al, 1997). Although these methods are sensitive, they typically require labeling of the antibodies or antigens, and this makes the assay process more complex, time consuming, and expensive.…”

Section: Introductionmentioning

confidence: 99%

A novel label-free amperometric immunosensor for carcinoembryonic antigen based on redox membrane

Shi

2011

Biosensors and Bioelectronics

102

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Chemiluminescence assay of alkaline phosphatase using cortisol-21 -phosphate as substrate and its application to enzyme immunoassays

Cited by 43 publications

References 10 publications

Chemiluminescent assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin

Chemiluminescent assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin

Sensitive electrochemical determination of α-fetoprotein using a glassy carbon electrode modified with in-situ grown gold nanoparticles, graphene oxide and MWCNTs acting as signal amplifiers

A novel label-free amperometric immunosensor for carcinoembryonic antigen based on redox membrane

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