Chemiluminescence detection of nine corticosteroids in liver

Abstract

A method for the determination of nine corticosteroids in bovine liver was developed. The liquid-liquid extraction method consisted in the addition of sodium hydroxide to the tissue sample followed by extraction with ethyl acetate. After centrifugation, the extract was evaporated to dryness and the residue redissolved in acetonitrile. The removal of fats was performed with n-hexane and the acetonitrile layer was evaporated. Afterwards, analysis of the extracts was performed using high-performance liquid chromatography with chemiluminescence detection employing luminol as reagent. The recovery curves, performed at five spiking levels (different for each corticosteroid), showed that recoveries of at least 70.5% could be obtained from liver. The chemiluminescence detection gave satisfactory results with respect to sensitivity, and the LOQ ranged from 0.13 ppb for prednisolone to 8.40 ppb for betamethasone. The maximum mean RSD was 4.8% and 7.3% for intra- and inter-day assay, respectively.

Methionine restriction effects on 11β-HSD1 activity and lipogenic/lipolytic balance in F344 rat adipose tissue

“…Corticosterone was extracted from AT (n 5 5) using the method described by Iglesias et al (34). Briefly, AT was homogenized in 1 mM NaOH (2 g/ml) and the homogenate was extracted with an equal volume of water-saturated ethyl acetate for 10 min at room temperature.…”

Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis—A review

“…After the extraction procedure, recoveries of at least 70.5% were obtained from liver. The method was also applied to the analysis of bovine urine [130].…”

Development of a solid phase extraction method for simultaneous determination of corticoids and tranquilizers in serum samples

“…Chromatographic separation of the target compound(s) has typically been carried out by high performance liquid chromatography (HPLC) [1, 10 -13] with a variety of detectors, including UV-Vis [1,10,12], chemiluminescence [2,9], mass spectrometry (MS) [7,14], and MS-MS [8,15]. Nevertheless, some methodologies involving the use of gas chromatography (GC) have also been described [6,16].…”

“…Classical procedures for the analysis of glucocorticoids or phenothiazines residues in matrices such as pharmaceutical preparations [2], urine [6], plasma [7], or liver [8,9] are laborious and time-consuming analytical protocols involving a number of steps for exhaustive extraction of the matrices with solvents of different polarity, and subsequent clean-up of the extracts prior to final determination. Chromatographic separation of the target compound(s) has typically been carried out by high performance liquid chromatography (HPLC) [1, 10 -13] with a variety of detectors, including UV-Vis [1,10,12], chemiluminescence [2,9], mass spectrometry (MS) [7,14], and MS-MS [8,15].…”