Chemiluminescence multichannel immunosensor for biodetection

Yacoub-George, Erwin; Meixner, Leonhard; Scheithauer, Waltraud; Koppi, Andreas; Drost, S.; Wolf, Hans; Danapel, Christine; Feller, Klaus A

doi:10.1016/s0003-2670(01)01167-9

Cited by 42 publications

(26 citation statements)

References 12 publications

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“…The main advantage of the array biosensor over existing technology is its ability to detect multiple analytes in multiple samples simultaneously on a single slide (19,26). Multianalyte sensors increasingly appear in the literature, but most have yet to look at matrices more complex than buffer (30,31). The ability to carry out multianalyte detection in complex samples is a clear advantage for screening food, water, or air samples for hazards either naturally occurring or deliberately introduced.…”

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confidence: 99%

Biosensor Detection of Botulinum Toxoid A and Staphylococcal Enterotoxin B in Food

Sapsford

Taitt

Loo

et al. 2005

Appl Environ Microbiol

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Immunoassays were developed for the simultaneous detection of staphylococcal enterotoxin B and botulinum toxoid A in buffer, with limits of detection of 0.1 ng/ml and 20 ng/ml, respectively. The toxins were also spiked and measured in a variety of food samples, including canned tomatoes, sweet corn, green beans, mushrooms, and tuna.The toxins staphylococcal enterotoxin B (SEB) and botulinum toxin A are responsible for food poisoning and have the potential to be used as biological warfare agents, with the current toxic dose for aerosol forms at 0.02 g/kg of body weight and 0.07 g/kg, respectively (3,4,28,29). There is need for a rapid method of monitoring food, water, and air samples for both natural and intentional contamination by these toxins. Here, we demonstrate the rapid, simultaneous dose-dependent detection of SEB and botulinum toxoid A (BotA), as measured using the Naval Research Laboratory array biosensor (7). The array biosensor has successfully been used for the detection of a variety of species, initially in buffer but increasingly in food and environmental matrices (16-22, 24, 26, 27).Here, biotinylated capture antibodies (10 g ml Ϫ1 in phosphate-buffered saline containing 0.05% Tween 20 [PBST]) were patterned on NeutrAvidin-functionalized slides using a poly(dimethyl)siloxane flow cell, as described previously (16). Each slide was patterned with two columns specific for chicken immunoglobulin Y (positive controls), five specific for BotA, and five for SEB. Twenty-minute sandwich immunoassays were performed essentially as described previously (19,22,24) using a cocktail of fluorescently labeled tracer antibodies (100 ng/ml Cy5-chicken immunoglobulin Y and 10 g/ml each of AlexaFluor647 anti-SEB and Cy5 anti-botulinum toxin A in PBST containing 1 mg/ml bovine serum albumin [PBSTB]) to detect bound targets (2, 24). Assays were developed with PBSTB for measurement of dose-response curves for both BotA and SEB simultaneously. The samples were spiked such that the concentrations of BotA decreased (0 to 500 ng/ml), while those of SEB increased (0 to 7.5 ng/ml). Four control samples were analyzed on each slide-two buffer controls containing only a single toxin (lanes 1 and 10) and an analogous set of spiked food matrices (lanes 2 and 9). A typical assay response is shown in Fig. 1, with a decreasing intensity of the BotA loci from top to bottom and increasing intensity for the SEB loci, corresponding to the concentration variation of the toxins. The different dynamic ranges used for the two toxins are a result of the different affinities of their respective antibodies. There is no apparent cross-reactivity between the species. The limit of detection (LOD), the lowest concentration giving 3 standard deviations above buffer-negative control values, was 20 ng/ml for BotA, although occasionally samples containing 5 ng/ml were positive by the same criteria. The SEB LOD was consistently found to be 0.1 ng/ml as previously reported (24). Although the LOD for BotA (20 ng/ml) is higher than that achieved by mouse bi...

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confidence: 99%

Biosensor Detection of Botulinum Toxoid A and Staphylococcal Enterotoxin B in Food

Sapsford

Taitt

Loo

et al. 2005

Appl Environ Microbiol

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“…The error bars represent the standard deviation. Yacoub-George et al, 2002). Indeed, the 10K-IDWG attains a 50-fold lower detection limit for SEB, a 10-fold lower detection limit for E. coli O157:H7, and a 20-fold lower detection limit for M13.…”

Section: Detection Limits and Calibration Curvesmentioning

confidence: 90%

“…The 10K-IDWG is a new approach to the demands of the German Armed Forces on a rapid multiplex BA immunodetector for the field. The first and second generation devices leading to the current technology of the 10K-IDWG, the MADAG, and the CL-MADAG, have been published previously (Koch et al, 2000;Yacoub-George et al, 2002). Both provide three channels only, allowing the simultaneous detection of up to three different biological agents in less than 1 h but without any controls.…”

Section: Introductionmentioning

confidence: 99%

Automated 10-channel capillary chip immunodetector for biological agents detection

Yacoub-George

Hell

Meixner

et al. 2007

Biosensors and Bioelectronics

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“…Various spectroscopic methods have been employed to identify and characterize bacterial endospores; these include terahertz, 1 infrared, [2][3][4][5] Raman, 6,7 photoacoustic, 8 luminescence, 9 and MALDI-TOF MS. [10][11] All these methods provide rapid analysis for identification, but some require significant sample preparation. FTIR spectroscopy is a non-destructive technology that can be used for bacterial identification by using small samples (e.g.…”

Section: Introductionmentioning

confidence: 99%

Infrared signatures to discriminate viability of autoclaved Bacillus spores

2011

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Optical methods can offer good sensitivity for detecting small amounts of chemicals and biologicals, and as these methods mature, are some of the few techniques that can offer true standoff detection. For detection of biological species, determining the viability is clearly important: Certain species of gram-positive bacteria are capable of forming endospores, specialized structures that arise when living conditions become unfavorable or little growth medium is available. Spores are also resistant to many chemicals as well as changes in heat or pH; such spores can remain dormant from months to years until more favorable conditions arise, resulting in germination back to the vegetative state. This persistence characteristic of bacterial spores allows for contamination of a surface (e.g. food or medical equipment) even after the surface has been nominally cleaned. Bacterial spores have also been used as biological weapons, as in the case of B. anthracis. Thus, having rapid analytical methods to determine a spore's viability after attempts to clean a given environment is crucial. The increasing availability of portable spectrometers may provide a key to such rapid onsite analysis. The present study was designed to determine whether infrared spectroscopy may be used to differentiate between viable vs. dead B. subtilis and B. atrophaeus spores. Preliminary results show that the reproducible differences in the IR signatures can be used to identify the viable vs. the autoclaved (dead) spores.

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Chemiluminescence multichannel immunosensor for biodetection

Cited by 42 publications

References 12 publications

Biosensor Detection of Botulinum Toxoid A and Staphylococcal Enterotoxin B in Food

Biosensor Detection of Botulinum Toxoid A and Staphylococcal Enterotoxin B in Food

Automated 10-channel capillary chip immunodetector for biological agents detection

Infrared signatures to discriminate viability of autoclaved Bacillus spores

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