Chemiluminescence of Luminol-Graphene Oxide for the Sensitive Detection of Puerarin in Biological Fluid and Chinese Gegen

Abstract

Graphene oxide (GO), one of the water-soluble derivatives of graphene, could initiate strong chemiluminescence (CL) emission of luminol in the absence of any oxidant, and then the CL intensity was inhibited by puerarin (PUE), a main component in the traditional Chinese medicine Gegen. Based on this, a novel CL method was established for the determination of PUE. This method showed a linear relationship between the CL signal and the logarithm of PUE concentration in the range 0.01-6.00 μM. The limit of detection was 2.83 nM and the relative standard deviation (RSD) was 1.94% for 11 determinations of 0.4 μM PUE. This method was successfully used for the determination of PUE in Gegen and human urine samples. The possible CL reaction mechanism was investigated by UV-vis, fluorescence, and CL spectra, as well as by some chemical experiments.

An Electrochemical Sensor Based on Ultra-Micro Activated Carbon for the Determination of Puerarin

“…The existing methods for puerarin analysis mainly include: high performance liquid chromatography, 5,6 capillary electrophoresis, 7 chemiluminescence, 8 Raman spectroscopy, 9 immunoassay, 10 and ultra-performance liquid chromatography-tandem mass spectrometry. 11 Although they have been accepted and could achieve the detection of a certain concentration of puerarin samples, but the complex sample preparation and strict operation requirements, as well as the need for special equipment and operating sites, leading to these methods for puerarin analysis cannot be widely used.…”