An amperometric biosensor was constructed on the basis of ruthenium purple-containing clay and glucose oxidase for the direct assay of glucose. These clay-modified electrodes were characterized by cyclic voltammetry and flow inection analysis techniques. The electrodes were very sensitive to the presence of glucose. Linear calibration curves between 10 M and 15 mM with an electrode sensitivity of 3.88 1.j.A/M and a detection limit of 10 iM were registered in aerated solutions (pH 5.1). Uric acid, ascorbic acid, and oxygen interfere very little with the detection of glucose. Long-term tests showed these clay electrodes were quite durable, e.g., the electrode sensitivity only decreases by ca. 25% over a period of six weeks.