ChemInform Abstract: Scalable Synthesis of N‐Acylaziridines from N‐Tosylaziridines.

Rubin, Heather N.; Cockrell, Jennifer; Morgan, Jeremy B.

doi:10.1002/chin.201404099

Cited by 2 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PNBA-OSu was synthesized as previously described: ESI analysis (positive ion) m / z 306 [M + ACN] + ; 1 H NMR (600 MHz, CDCl 3 ) δ 8.37 (d, J = 9.0 Hz, 2H), 8.33 (d, J = 9.0 Hz, 2H), 2.94 (s, 4H). A solution of PNBA-OSu (700 mg, 2.65 mmol) in ACN (20 mL) was added dropwise (with stirring) to a solution of histamine dihydrochloride (460 mg, 2.5 mmol) in 1 M NaOH (10 mL).…”

Section: Methodsmentioning

confidence: 99%

Synthesis of Novel Haptens and Development of an Enzyme-Linked Immunosorbent Assay for Quantification of Histamine in Foods

Luo

Yang

et al. 2014

J. Agric. Food Chem.

View full text Add to dashboard Cite

Novel haptens were designed and synthesized to prepare antibodies against free histamine, but none resulted in producing suitable antibodies for developing an enzyme-linked immunosorbent assay (ELISA). However, an antiserum was obtained having high specificity and affinity to p-nitrobenzoylated histamine (NPHA), which can be easily formed from reaction between histamine and p-nitrobenzoic acid N-hydroxysuccinimide ester (PNBA-OSu) under mild conditions. Based on rabbit polyclonal antibodies, a competitive indirect ELISA (ciELISA) for histamine determination in foods was developed. After ciELISA and derivatization optimization, the assay showed good sensitivity, with limits of detection of 1.8 mg/kg, 93.6 μg/L, and 93.6 μg/kg in fish, red wine, and yoghurt, respectively, with negligible cross-reactivity with related biogenic amines and amino acids. Average recovery of histamine in fortified food samples ranged from 80.9% to 110.1% with coefficients of variation below 16.3%. Good correlation between the ciELISA and liquid chromatography-tandem mass spectrometry was obtained for spiked food samples.

show abstract

Section: Methodsmentioning

confidence: 99%

Synthesis of Novel Haptens and Development of an Enzyme-Linked Immunosorbent Assay for Quantification of Histamine in Foods

Luo

Yang

et al. 2014

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Currently there are mainly block data processing, Directed Acyclic Graph(DAG) directed acyclic graph structures, sharding techniques, and concurrent solutions. Jeremy Rubin, M. Naik et al [8] proposed a Bitcoin improvement scheme that converts Bitcoin scripts into Merkle trees of their Merkelized Abstract Syntax Trees(MAST) branches, unused scripts can be removed from the block, and when the Merkle proof returns True, the transaction proponent needs to provide a proof of the Merkle tree with the missing script branch in order to prove spending bitcoins. Sompolinsky et al [9] proposed a PoW permissionless blockchain PHANTOM based on the DAG structure, which promotes the directed acyclic graph of bit blocks to Satoshi Nakamoto's blockchain.…”

mentioning

confidence: 99%

GT-NRSM: efficient and scalable sharding consensus mechanism for consortium blockchain

Shen

Yu³

et al. 2023

J Supercomput

View full text Add to dashboard Cite

Blockchain is an innovative application of distributed storage, consensus mechanism, cryptographic algorithm and other computer technologies. As the underlying architecture of blockchain, consensus mechanism is the key to realize service-oriented applications of blockchain in terms of its security, efficiency and scalability optimization. In some high complexity consensus mechanism such as Practical Byzantine Fault Tolerance (PBFT), throughput is severely reduced as the number of nodes increases, and even in low complexity algorithms such as Raft, the load on leader is severely affected as the network size increases, which affecting consensus efficiency. To solve these problems, in this paper, we propose a node reliable shard model based on guarantee tree (GT-NRSM) that achieves high scalability while ensuring a certain degree of decentralization and security based on consortium blockchain. Firstly, we design a guarantee mechanism to represent the trust relationship between nodes, and then we design a reliable node selection strategy based on the guarantee mechanism to evaluate the node guarantee results and consensus behavior, determine the node trust status, and identify Article Title malicious nodes and select a list of trusted leaders. Secondly, we propose a Dual-Leaders supervision mechanism, where deputy detects the heartbeat of leader while the deputy activity is detected by consensus nodes. Finally, we use guarantee mechanism and reliable node selection strategy to design a network partitioning method to achieve high concurrent consensus for multiple partitions and greatly improve the consensus efficiency. Subsequent experiments show that the throughput of the proposed algorithm improves by 48% over Raft and is much higher than PBFT, which has higher throughput and lower consensus latency.

show abstract

ChemInform Abstract: Scalable Synthesis of N‐Acylaziridines from N‐Tosylaziridines.

Cited by 2 publications

References 1 publication

Synthesis of Novel Haptens and Development of an Enzyme-Linked Immunosorbent Assay for Quantification of Histamine in Foods

Synthesis of Novel Haptens and Development of an Enzyme-Linked Immunosorbent Assay for Quantification of Histamine in Foods

GT-NRSM: efficient and scalable sharding consensus mechanism for consortium blockchain

Contact Info

Product

Resources

About