Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex

Mousavi, Mahdie; Koosha, Fereshteh; Neshasteh-Riz, Ali

doi:10.1016/j.heliyon.2023.e13847

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…Viable cells were evaluated using the MTT assay and ow cytometry. X-ray radiation signi cantly decreased the viability of U87MG cells compared to HGF (23). Radiation therapy and accelerated hypofractionation can in uence mitochondrial function negatively and improve cell radiosensitivity (24).…”

Section: Discussionmentioning

confidence: 99%

Investigating the efficacy of radiotherapy and photodynamic treatments using gold nanoclusters carrying 5-aminolevulinic acid on the K562 cell line

Khosravi,

Saeedi,

Goodarzi

et al. 2024

Preprint

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Background In this study, a nanosystem of gold particles functionalized with aminolevulinic acid was used in combination with radiotherapy and photodynamic therapy on the K562 cell line to increase treatment efficacy. One of the benefits of performing this project is to investigate the effects of two combined methods—radiotherapy and photodynamic therapy—on cancer cell lines, which are expected to be useful in cancer treatment. Methods The MTT test was used to assess cell viability. First, approximately 10,000 cells were seeded in 96-well plates. The cells were kept in an incubator for 24 hours and then treated with 1.25, 0.625 or 0.3125 mg/mL nanoparticles. In the next step, the plates were treated with a laser for 60 seconds and then 2 or 4 Gy doses of X-rays with an energy of 6 megaelectron volts or vice versa. Cell counting was performed using a slide hemocytometer. To measure MTT levels, commercial kits were used according to the manufacturer’s instructions. Results The results of this study showed that the most effective treatment method was laser irradiation before X-rays were applied at a dose of 4 Gy to K562 cells in the presence of gold nanoparticles (4 Gy X-ray + nano + laser before), for which the average survival rate was 27.0120%. Conclusions The results of this study showed that gold nanoparticles can be investigated and used as a new treatment option for all types of cancer as a complement to photodynamic therapy and radiation therapy.

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Section: Discussionmentioning

confidence: 99%

Investigating the efficacy of radiotherapy and photodynamic treatments using gold nanoclusters carrying 5-aminolevulinic acid on the K562 cell line

Khosravi,

Saeedi,

Goodarzi

et al. 2024

Preprint

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“…The antiproliferative effects of selected stone fruit juices on U87-MG and GBM43 glioblastoma cells were compared with TMZ, the current standard chemotherapeutic drug of choice for the treatment of patients with glioblastoma. These two types of cell are commonly employed in glioblastoma research due to their ability to provide insights into the biology of glioblastoma, cancer cell growth, invasiveness, and suitability for testing potential therapeutic interventions [27][28][29][30][31][32]. As previously mentioned, there has been a growing interest in exploring active ingredients from natural sources for the prevention and treatment of malignant tumors [15,16].…”

Section: Discussionmentioning

confidence: 99%

“…They are applied to the investigation of diverse aspects of the biology of glioblastoma, including growth of tumor, its invasiveness, and its response to selected therapies. Consequently, these cells are utilized in the testing of various drugs and preclinical studies [27][28][29]. The source and manipulation of the cells are key factors in determining the specific properties of GBM43 cells.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Compounds, Antioxidant Activity, and Antiproliferative Potential on Glioblastoma Cells of Selected Stone Fruit Juices

Raucher,

Rowsey,

Hinson

et al. 2024

Processes

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Glioblastoma presents one of the most formidable challenges in cancer treatment, remaining persistently incurable. There is a pressing need to explore less toxic alternatives, particularly natural remedies that could be applied in glioblastoma therapy. The aim of this research is to investigate the antiproliferative potential of selected stone fruit juices—tart cherry (Prunus cerasus), cornelian cherry (Cornus mas), and blackthorn (Prunus spinosa)—on U87-MG and GBM43 glioblastoma cells. Their effects were compared with temozolomide (TMZ), the current standard treatment. Additionally, the juices were assessed for their bioactive compounds and antioxidant potential. Unlike the other two juices, blackthorn juice did not exhibit an antiproliferative effect on U87-MG cells. However, all three juices, including blackthorn, demonstrated antiproliferative potential against TMZ-resistant GBM43 cells. Cornelian cherry exhibited an even stronger inhibitory effect than TMZ. This observation correlated with cornelian cherry being rich in iridoids, while tart cherry juice contained significant amounts of anthocyanins and proanthocyanidins. This research sheds light on the potential of cornelian cherry juice as a source of bioactive compounds with antiproliferative effects against glioblastoma cells, particularly TMZ-resistant GBM43 cells. Further research is warranted to explore the potential development of these compounds into therapeutic agents, either as single entities or in combination therapies for glioblastoma treatment.

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“…The results were obtained using three independent experiments. The percentage of cell death was calculated using the following formula 72 : …”

Section: Methodsmentioning

confidence: 99%

Comprehensive antifungal investigation of green synthesized silver nanoformulation against four agriculturally significant fungi and its cytotoxic applications

Singh,

Kumar,

Nayal

et al. 2024

Sci Rep

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The present study reports the green synthesis of silver nanoparticles (AgNPs) in powder form using the leaf extract of Azadirachta indica. The synthesis of AgNPs was confirmed by UV–vis spectroscopy, FTIR, XRD, FESEM, and EDX. The synthesized AgNPs were in a powdered state and dispersed completely in 5% polyethylene glycol (PEG) and demonstrated prolonged shelf life and enhanced bioavailability over a year without any aggregation. The resulting silver nanoformulation demonstrated complete inhibition against Sclerotinia sclerotiorum and Colletotrichum falcatum and 68% to 80% inhibition against Colletotrichum gloeosporioides and Rhizoctonia solani respectively, at 2000 ppm. The EC50 values determined through a statistical analysis were 66.42, 157.7, 19.06, and 33.30 ppm for S. sclerotiorum, C. falcatum, C. gloeosporioides, and R. solani respectively. The silver nanoformulation also established significant cytotoxicity, with a 74.96% inhibition rate against the human glioblastoma cell line U87MG at 250 ppm. The IC50 value for the cancerous cell lines was determined to be 56.87 ppm through statistical analysis. The proposed silver nanoformulation may be used as a next-generation fungicide in crop improvement and may also find application in anticancer investigations. To the best of our knowledge, this is also the first report of silver nanoformulation demonstrating complete inhibition against the economically significant phytopathogen C. falcatum.

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Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex

Cited by 5 publications

References 39 publications

Investigating the efficacy of radiotherapy and photodynamic treatments using gold nanoclusters carrying 5-aminolevulinic acid on the K562 cell line

Investigating the efficacy of radiotherapy and photodynamic treatments using gold nanoclusters carrying 5-aminolevulinic acid on the K562 cell line

Bioactive Compounds, Antioxidant Activity, and Antiproliferative Potential on Glioblastoma Cells of Selected Stone Fruit Juices

Comprehensive antifungal investigation of green synthesized silver nanoformulation against four agriculturally significant fungi and its cytotoxic applications

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