Chemokine Receptor 2 (CXCR2) Gene Polymorphisms and Their Association with the Risk of Developing Peri-Implantitis in Chinese Han Population

Qi, Yuesun; Li, Cheng; Du, Yimin; Lin, Jichao; Li, Nan; Yu, Youcheng

doi:10.2147/jir.s304261

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…As the major receptor of CXCL8, CXCR2 plays a critical role in the regulation of neutrophil homeostasis (Stadtmann & Zarbock, 2012). This may help explain the association between CXCR2 gene polymorphisms and peri-implantitis susceptibility in the Chinese Han population seen in a previous report (Qi et al, 2021).…”

Section: Discussionmentioning

confidence: 84%

Single‐cell sequencing identifies inflammation‐promoting fibroblast–neutrophil interaction in peri‐implantitis

Mo,

Lai,

Huang

et al. 2023

J Clinic Periodontology

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AimTo reveal the cellular composition and molecular environment of the periodontal and peri‐implant inflammatory infiltrates through a single‐cell sequencing technique, which may explain the pathological difference between these two diseases. A special focus was placed on the phenotypes and potential roles of neutrophils and fibroblasts in peri‐implant/periodontal tissue immunity.Materials and MethodsHigh‐throughput single‐cell transcriptomic profiling of peri‐implant tissues from patients with peri‐implantitis as well as periodontal tissues from patients with periodontitis and healthy donors was performed. Immunofluorescence analysis was carried out to further validate the identified cell subtypes and their involvement in peri‐implantitis and periodontitis.ResultsBased on our single‐cell resolution analysis, a quantified proportional increase of neutrophil (Neu) subtypes was shown in peri‐implantitis. Among these, a predominance of Neutro_CXCR2 was revealed. We also found the involvement of inflammation‐promoting fibroblasts as well as a predominance of CXCL8+ fibroblast–CXCR2+ neutrophil interaction in peri‐implantitis.ConclusionsOur study indicated that the predominance of CXCL8+ fibroblast–CXCR2+ neutrophil interaction might underline the enhanced host response in peri‐implantitis compared with periodontitis. This information offers a molecular basis by which fibroblast and neutrophil subtypes might be diagnostically and therapeutically targeted in peri‐implantitis.

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Section: Discussionmentioning

confidence: 84%

Single‐cell sequencing identifies inflammation‐promoting fibroblast–neutrophil interaction in peri‐implantitis

Mo,

Lai,

Huang

et al. 2023

J Clinic Periodontology

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“…Although the critical role of immune reaction in the development of peri-implantitis has been established, the specific molecular mechanisms are still unclear. Related studies have confirmed that immune reactions are closely related to the occurrence and development of periimplantitis [11][12][13][14]. Exploring immune-related genes in periimplantitis is of great significance for the molecular diagnosis and immunotherapy of this condition.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Immune Infiltration and Key Genes in Peri-Implantitis Using Bioinformatics and Molecular Biology Approaches

Meng,

Han,

Zhang

et al. 2024

Med Sci Monit

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Background:Peri-implantitis is the main cause of failure of implant treatment, and there is little research on its molecular mechanism. This study aimed to identify key biomarkers and immune infiltration of peri-implantitis using a bioinformatics method. Material/Methods: Three Gene Ontology (GO) gene expression profiles were selected from the Gene Expression Omnibus. Differentially expressed genes (DEGs) were identified by the LIMMA package, and functional correlations of DEGs were analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Information on immune-related genes was obtained from ImmPort (https://www.immport.org) and InnateDB (http://www.innatedb.com).Immune-related DEGs were screened by least absolute shrinkage and selection operator (LASSO) and support vector machine-recursive feature elimination (SVM-RFE). The single-sample Gene Set Enrichment Analysis algorithm was used to analyze immune cell infiltration in gingival tissue between peri-implantitis and normal controls. Finally, results of bioinformatics analysis were verified by qPCR. Results:A total of 398 DEGs were identified, of which 96 were immune-related. Enrichment analysis showed these genes were enriched in inflammatory response, leucocyte chemotaxis, immune response-regulating signaling pathway, and cell activation. Seven key genes were selected by LASSO and SVM-RFE. Receiver operating characteristic curve results showed these genes had excellent diagnostic efficacy. Results of qPCR showed significant differences in the expression of these genes. Conclusions:Differences in key genes and immune infiltration between peri-implantitis and gingival tissues of normal controls may provide new insights into the development of peri-implantitis. Elucidating the difference in immune infiltration between peri-implantitis tissues and normal tissues will help to understand the development of peri-implantitis.

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Molecular Determination of Tumor Necrosis Factor-alpha, Interleukin-8, Interleukin-10, and C-X-C Chemokine Receptor-2 Genetic Variations and their Association with Disease Susceptibility and Mortality in COVID-19 Patients

Alsayed,

Mir,

Mir

et al. 2024

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Background: Altered cytokine levels have been associated with poor outcomes among COVID-19 patients. TNF-α, IL-8 and IL-10 are key cytokines in COVID-19 pathogenesis, and CXCR-2 is a major chemokine receptor involved in inflammatory response. Polymorphisms in the genes of these proteins are proposed to influence disease outcomes. In this study, we aimed to find out the association of genetic polymorphisms in TNF-α, IL-8, IL-10 and CXCR-2 genes with susceptibility to and mortality of COVID-19. Methods: The present case-control study was conducted on 230 subjects, among whom 115 were clinically diagnosed and RT-PCR-confirmed COVID-19 patients and 115 healthy control subjects. The polymorphisms in TNFα -308 G>A (rs1800629), IL-8 -251T>A (rs4073), CXCR2 +785 C>T (rs2230054) genes were detected by ARMS -PCR assay whereas for IL-10 (-1082 G>A), rs1800896 G>A allele-specific PCR assay was used and their association with COVID-19 susceptibility and mortality was estimated by multivariate analysis. The results were analyzed for risk of infection and mortality through different inheritance models. Results: Frequencies of TNF-α rs1800629 GA and AA, IL-8 rs4073 TA and AA, IL-10 (-1082 G>A), rs1800896 GA and GG, and CXCR2 rs2230054 CT genotypes were significantly higher in COVID-19 patients compared to the control group (p < 0.05). Furthermore, COVID-19 patients had a higher frequency of the polymorphic A allele of TNF-α, the A allele of IL-8, the G allele of IL-10, and the T allele of CXCR2. The risk of susceptibility to COVID-19 was significantly associated with TNF-α rs1800629 GA and GA+AA genotypes and the A allele, IL-8 rs4073 TA and AA genotypes and A allele, IL-10 rs1800872 GA and CC genotypes and C allele, and CXCR2 rs2230054 CT and CT+CC genotypes. TNF-α-GA and AA genotypes and A allele, IL-8 TA and AA genotypes and A allele and CXCR-2 CC and CT genotypes have significant associations with mortality risk in COVID-19 patients, while GA and GG genotypes of the IL-10 are shown to confer significant protection against mortality from COVID-19. Conclusion: The findings of this study provide important insights into the COVID-19 disease and susceptibility risk. The polymorphisms in TNFα -308 G>A (rs1800629), IL-8 -251T>A (rs4073), IL-10 (-1082 G>A), rs1800896 and CXCR2 +785 C>T (rs2230054) are associated with the risk of susceptibility to COVID-19 and with mortality in COVID-19 patients. Further studies with larger sample sizes are necessary to confirm our findings.

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Chemokine Receptor 2 (CXCR2) Gene Polymorphisms and Their Association with the Risk of Developing Peri-Implantitis in Chinese Han Population

Cited by 4 publications

References 26 publications

Single‐cell sequencing identifies inflammation‐promoting fibroblast–neutrophil interaction in peri‐implantitis

Single‐cell sequencing identifies inflammation‐promoting fibroblast–neutrophil interaction in peri‐implantitis

Comprehensive Analysis of Immune Infiltration and Key Genes in Peri-Implantitis Using Bioinformatics and Molecular Biology Approaches

Molecular Determination of Tumor Necrosis Factor-alpha, Interleukin-8, Interleukin-10, and C-X-C Chemokine Receptor-2 Genetic Variations and their Association with Disease Susceptibility and Mortality in COVID-19 Patients

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