Chemokines induced by PEDV infection and chemotactic effects on monocyte, T and B cells

Yuan, Chen; Sun, Lidan; Chen, Ligong; Guo, Haiyang; Yao, Zuojun; Wang, Yawen; Zhu, Weihuang; Li, Tanqing; Song, Qinye; Li, Huanrong

doi:10.1016/j.vetmic.2022.109599

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…Our RNA-seq analysis also showed that PEDV-infected HepG2, Hep3B217, and SNU387 could induce several cytokines that were involved in inflammatory cells' expansion and migration implying that those cell lines were suitable in vitro models for studying the innate immune response of PEDV infection. It has been reported that the expression levels of chemokines including IL-8, CXCL9, CXCL10, and CXCL13 are significantly increased in the ileum of PEDV-infected piglets [40]. Our RNA-seq analysis found that the most upregulated chemokine genes in all these cell lines were CXCL1, CXCL2, CXCL3, CXCL5, CXCL8 (IL8), CCL20, IL6, and CXCL6, and most of these chemokines were not reported before.…”

Section: Discussioncontrasting

confidence: 46%

Identification and Characterization of Cell Lines HepG2, Hep3B217 and SNU387 as Models for Porcine Epidemic Diarrhea Coronavirus Infection

Luo

et al. 2022

Viruses

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Porcine epidemic diarrhea virus (PEDV), a member of the genera alphacoronavirus, causes acute watery diarrhea and dehydration in suckling piglets and results in enormous economic losses in the swine industry worldwide. Identification and characterization of different cell lines are not only invaluable for PEDV entry and replication studies but also important for the development of various types of biological pharmaceuticals against PEDV. In this study, we present an approach to identify suitable permissive cell lines for PEDV research. Human cell lines were screened for a high correlation coefficient with the established PEDV infection model Huh7 based on RNA-seq data from the Cancer Cell Line Encyclopedia (CCLE). Experimentally testing permissiveness towards PEDV infection, three highly permissive human cell lines, HepG2, Hep3B217, and SNU387 were identified. The replication kinetics of PEDV in HepG2, Hep3B217, and SNU387 cells were similar to that in Vero and Huh7 cells. Additionally, the transcriptomes analysis showed robust induction of transcripts associated with the innate immune in response to PEDV infection in all three cell lines, including hundreds of inflammatory cytokine and interferon genes. Moreover, the expression of inflammatory cytokines and interferons were confirmed by qPCR assay. Our findings indicate that HepG2, Hep3B217, and SNU387 are suitable cell lines for PEDV replication and innate immune response studies.

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Section: Discussioncontrasting

confidence: 46%

Identification and Characterization of Cell Lines HepG2, Hep3B217 and SNU387 as Models for Porcine Epidemic Diarrhea Coronavirus Infection

Luo

et al. 2022

Viruses

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“…In this study, lambs fed with alfalfa hay showed a significant downregulation of DDX58 ( 60 ), CXCL9 ( 61 ), and SERPINE2 ( 62 ) in rumen tissue. This suggests a potential reduction in epithelial inflammation levels and an augmentation of immune capabilities.…”

Section: Discussionmentioning

confidence: 58%

Ruminal microbiota-host crosstalks promote ruminal epithelial development in neonatal lambs with alfalfa hay introduction

Bian,

Yu,

Cheng

et al. 2024

mSystems

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Ruminal microbiota is gradually established after birth, while microbiota maturation could be highly diverse because of varied solid dietary accessibility. However, how the ruminal microbiota accreted from postnatal hay diets alters rumen epithelial development, and how this affects animal health remains largely unknown. Here, neonatal lambs were introduced to starchy corn-soybean starter or corn-soybean starter + alfalfa hay (AH) to investigate the influences of early life ruminal microbiome on rumen epithelial development using integrated 16s rRNA sequencing-metagenome-transcriptome approaches. The results showed that AH introduction elevated average daily weight gain, rumen weight and volume, rumen epithelial papillae length, and rumen muscle layer thickness. Meanwhile, the relative abundance of fibrolytic bacteria ( Christensenellaceae R-7 group, Prevotellaceae UCG-001, and Succinivibrio ), acetate producer ( Acetitomaculum and Mitsuokella), and propionate producer Succiniclasticum was increased in the rumen content by AH supplementation ( P < 0.05). Moreover, AH introduction decreased the relative abundance of total CAZymes, CBM, and GH and increased the abundance of KO genes related to volatile fatty acid (VFA) generation in the rumen content. AH lambs had a higher relative abundance of Succiniclasticum , Megasphaera, Succinivibrio , and Suttonella ( P < 0.05), while a lower relative abundance of Cloacibacillus , Desulfovibrio , Dialister , Intestinimonas , Parabacteroides , and Pseudoscardovia ( P < 0.05) in the rumen epithelial samples. Furthermore, these alterations in ruminal microbial structure and function resulted in ruminal epithelial cell proliferation and development pathways activation. In summary, AH introduction benefited ruminal fiber degradation and VFA generation bacteria colonization and promoted ruminal epithelial development. These findings provide new insights into ruminal microbial–host interactions in the early life. IMPORTANCE While it is established that a fiber-rich diet promotes rumen development in lambs, further research is needed to investigate the precise response of rumen microbiota and epithelium to high-quality alfalfa hay. Here, we observed that the inclusion of alfalfa hay led to a discernible alteration in the developmental trajectory of the rumen. Notably, there was a favorable shift in the rumen's volume, morphology, and the development of rumen papillae. Furthermore, ruminal microbial structure and function resulted in ruminal epithelial cell proliferation and development pathways activation, collectively provide compelling evidence supporting the capacity of alfalfa hay to enhance rumen development and health through ruminal micrbiota-host crosstalks. Our findings elucidate the functional response of the rumen to alfalfa hay introduction, providing new insights into strategies for promoting healthy development of the rumen in young ruminants.

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“…Driven by the chemokine CCL2 secreted by intestinal epithelial cells infected with PEDV, the number of lymphocytes entering the intestine increased ( 33 ). CXCL9 secreted by PEDV-infected IPEC-J2 cells significantly regulated the number of T-cell subpopulations in the peripheral blood cells of piglets, and high expression of CXCL13 significantly increased the number of B-cell migrations ( 34 ). The synergistic role of CCL25 and CCL28 in translocating IgA cells into the intestine had been demonstrated in mice, and pigs immunized with CCL25/CCL28 adjuvant virus-like particles showed excellent immune protection against PEDV ( 26 , 35 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics yields valuable information regarding the response mechanisms of Chinese Min pigs infected with PEDV

Li,

Zhou,

Zhang

et al. 2023

Front. Vet. Sci.

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Porcine epidemic diarrhea virus (PEDV) causes porcine epidemic diarrhea (PED), a highly infectious disease, which has resulted in huge economic losses for the pig industry. To date, the pathogenic and immune response mechanism was not particularly clear. The purpose of this study was to investigate the pathogenic and immune responses of pigs infected with PEDV.In this study, 12 Min pigs were randomly selected without taking colostrum. At 3 days old, eight piglets were infected with 1 mL of PEDV solution (10 TCID50/ml), and the remaining four piglets were handled by 1 mL of 0.9% normal saline. Within the age of 7 days old, four piglets died and were considered as the death group. Correspondingly, four alive individuals were classified into the resistance group. Tissues of the duodenum, jejunum, ileum, colon, cecum, and rectum of piglets in the three groups were collected to measure the PEDV content. Additionally, the jejunum was used for the measurements and analyses of Hematoxylin-eosinstaining (HE), immunohistochemical sections, and transcriptomics. The phenotypes of Min piglets infected with PEDV showed that the viral copy numbers and jejunal damage had significant differences between the death and resistance groups. We also observed the transcriptome of the jejunum, and the differentially expressed (DE) analysis observed 6,585 DE protein-coding genes (PCGs), 3,188 DE long non-coding RNAs (lncRNAs), and 350 DE microRNAs (miRNAs), which were mainly involved in immune response and metabolic pathways. Furthermore, the specific expressed molecules for each group were identified, and 97 PCGs,108 lncRNAs, and 51 miRNAs were included in the ceRNA-regulated networks. By weighted gene co-expression network analysis (WGCNA) and transcription factor (TF) prediction, 27 significant modules and 32 significant motifs (E-value < 0.05) annotated with 519 TFs were detected. Of these TFs, 53 were DE PCGs. In summary, the promising key PCGs, lncRNAs, and miRNAs related to the pathogenic and immunological response of pigs infected with PEDV were detected and provided new insights into the pathogenesis of PEDV.

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Chemokines induced by PEDV infection and chemotactic effects on monocyte, T and B cells

Cited by 8 publications

References 34 publications

Identification and Characterization of Cell Lines HepG2, Hep3B217 and SNU387 as Models for Porcine Epidemic Diarrhea Coronavirus Infection

Identification and Characterization of Cell Lines HepG2, Hep3B217 and SNU387 as Models for Porcine Epidemic Diarrhea Coronavirus Infection

Ruminal microbiota-host crosstalks promote ruminal epithelial development in neonatal lambs with alfalfa hay introduction

Transcriptomics yields valuable information regarding the response mechanisms of Chinese Min pigs infected with PEDV

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